Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates DOI Creative Commons
Andrey Shelenkov,

Yulia Mikhaylova,

Shushanik Voskanyan

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1314 - 1314

Published: May 17, 2023

Klebsiella pneumoniae, a member of the Enterobacteriaceae family, has become dangerous pathogen accountable for large fraction various infectious diseases in both clinical and community settings. In general, K. pneumoniae population been divided into so-called classical (cKp) hypervirulent (hvKp) lineages. The former, usually developing hospitals, can rapidly acquire resistance to wide spectrum antimicrobial drugs, while latter is associated with more aggressive but less resistant infections, mostly healthy humans. However, growing number reports last decade have confirmed convergence these two distinct lineages superpathogen clones possessing properties both, thus imposing significant threat public health worldwide. This process horizontal gene transfer, which plasmid conjugation plays very important role. Therefore, investigation structures ways plasmids spread within between bacterial species will provide benefits prevention measures against powerful pathogens. this work, we investigated multidrug-resistant isolates using long- short-read whole-genome sequencing, allowed us reveal fusion IncHI1B/IncFIB ST512 capable simultaneously carrying hypervirulence (iucABCD, iutA, prmpA, peg-344) determinants (armA, blaNDM-1 others), obtain insights their formation transmission mechanisms. Comprehensive phenotypic, genotypic phylogenetic analysis isolates, as well repertoire, was performed. data obtained facilitate epidemiological surveillance high-risk development strategies them.

Language: Английский

Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades Infecciosas y Microbiología Clínica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Genome drafting of nosocomial infection CRE Klebsiella pneumoniae confirming resistance to colistin and eravacycline, carrying blaNDM-1, mcr-1, and blaKPC-2, in neonatology from November to December 2023 DOI Creative Commons
Xiaoming Zhou,

Kailash Wagh,

Guangxin Lv

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 27, 2025

Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a critical pathogen in healthcare settings, associated with high mortality due to its extensive antibiotic resistance. In this study, we report an outbreak of CRKP neonatal intensive care unit (NICU) within 200-bed tertiary hospital. The main goal study was characterize the phenotypic and genomic profiles isolates involved gain insights into their resistance mechanisms transmission dynamics NICU. Methods conducted between November December 2023 5-bed Monthly surveillance cultures were performed monitor colonization infection multidrug-resistant organisms. obtained from blood nasal swabs affected neonates. Identification antimicrobial susceptibility testing initially using Vitek ® 2 system N-395 card further confirmed by 16S rRNA sequencing. Whole-genome sequencing (WGS) (AMR) profiling identify genes virulence factors. For genetic analysis, both Illumina short-read Nanopore long-read used, followed hybrid assembly for enhanced genome resolution. Plasmid gene determined AMRFinder PlasmidFinder databases. Results A total three (designated Kp1, Kp2, Kp3) identified. Kp1 Kp2 belonged sequence type (ST) ST23 genetically near-identical, differing single allele, while Kp3 distinct type, ST2096, 245 allelic differences Kp2. All resistant colistin carried genes, including mcr-1 bla NDM-1, KPC2 confirming carbapenem Efflux pump aminoglycoside also detected, providing multifaceted defence against antibiotics. analysis identified several incompatibility groups (IncFI, IncHI, IncFIB, IncX), indicating potential horizontal transfer determinants. Conclusion This highlights complexity outbreaks care, exhibiting that complicate treatment. plasmid suggest these strains are reservoirs emphasizing need strict control ongoing surveillance. challenges increase risk treatment failures mortality, underscoring importance prevention novel therapeutic strategies.

Language: Английский

Citations

1
Genomic analysis of extensively drug resistant (XDR) Klebsiella pneumoniae high-risk clone ST14 co-harboring blaNDM and blaOXA-48 recovered from Saudi Arabia DOI Creative Commons
Ibrahim Alzahrani, Ahmed Aljabri, Wafaa Alhazmi

et al.

Journal of Infection and Public Health, Journal Year: 2024, Volume and Issue: 17(4), P. 669 - 675

Published: Feb. 22, 2024

This study presents a comprehensive genomic analysis of NDM and OXA-48-producing Klebsiella pneumoniae in the Western region Saudi Arabia, traversed by tens millions Muslims from various countries annually. significant influx visitors invariably leads to spread diversity MDR bacteria.

Language: Английский

Citations

6
Coexistence of blaNDM-5, blaCTX-M-15, blaOXA-232, blaSHV-182 genes in multidrug-resistant K. pneumoniae ST437-carrying OmpK36 and OmpK37 porin mutations: First report in Italy DOI Creative Commons

Sascia Di Marcantonio,

Mariagrazia Perilli, Giovanni Alloggia

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2024, Volume and Issue: 37, P. 24 - 27

Published: Feb. 24, 2024

K. pneumoniae is a common cause of severe hospital-acquired infections. In the present study, we have characterised whole-genome two ST437 belonging to clonal complex CC258. The sequencing was performed by MiSeq Illumina, with 2 × 300bp paired-end run. ResFinder 4.4.2 used detect acquired antimicrobial resistance genes (ARGs) and chromosomal mutations. Mobile genetic elements (plasmids ISs) were identified MobileElementFinder v1.0.3. genome also assigned ST using MLST 2.0.9. Virulence factors detected Factor Database (VFDB). KPNAQ_1/23 KPNAQ_2/23 strains, isolated from urine samples hospitalised patients, showed most antibiotics, including ceftazidime-avibactam, ceftolozane-tazobactam, meropenem-vaborbactam combinations. Both strains susceptible only cefiderocol. Multiple mechanisms identified. Resistance β-lactams due presence NDM-5, OXA-232, CTX-M-15, SHV-182 β-lactamases, OmpK36 OmpK37 porin fluoroquinolones mediated mutations in acrR, oqxAB efflux pumps, bifunctional gene aac(6')-Ib-cr. different virulence makes these high-risk clones.

Language: Английский

Citations

6
Genomic insights unveil the plasmid transfer mechanism and epidemiology of hypervirulent Klebsiella pneumoniae in Vietnam DOI Creative Commons
Quynh Nguyen,

Yen Thi Phuong Nguyen,

Tuyen Thanh Ha

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 17, 2024

Hypervirulent Klebsiella pneumoniae (hvKp) is a significant cause of severe invasive infections in Vietnam, yet data on its epidemiology, population structure and dynamics are scarce. We screened hvKp isolates from patients with bloodstream (BSIs) at tertiary infectious diseases hospital Vietnam healthy individuals, followed by whole genome sequencing plasmid analysis. Among 700 BSI-causing Kp strains, 100 (14.3%) were hvKp. Thirteen identified 350 rectal swabs adults; none 500 children. The genetically diverse, encompassing 17 sequence types (STs), predominantly ST23, ST86 ST65. the 113 isolates, 14 (12.6%) carried least one antimicrobial resistance (AMR) gene, largely mediated IncFII, IncR, IncA/C plasmids. Notably, acquisition AMR conjugative plasmids facilitated horizontal transfer non-conjugative virulence between K. strains. Phylogenetic analysis demonstrated BSIs human carriage clustered together, suggesting role intestinal transmission. Enhanced surveillance crucial to understand factors driving transmission for informing preventive measures. Furthermore, we advocate clinical use our molecular assay diagnosing guide effective management.

Language: Английский

Citations

6
The emergence of highly resistant and hypervirulent Klebsiella pneumoniae CC14 clone in a tertiary hospital over 8 years DOI Creative Commons
Sharif Hala, Mohammed Malaikah, Jiayi Huang

et al.

Genome Medicine, Journal Year: 2024, Volume and Issue: 16(1)

Published: April 18, 2024

Klebsiella pneumoniae is a major bacterial and opportunistic human pathogen, increasingly recognized as healthcare burden globally. The convergence of resistance virulence in K. strains has led to the formation hypervirulent multidrug-resistant with dual risk, limiting treatment options. clones are known emerge locally spread Therefore, an understanding dynamics evolution emerging hospitals warranted prevent future outbreaks. In this study, we conducted in-depth genomic analysis on large-scale collection 328 (MDR) recovered from 239 patients single hospital western coastal city Jeddah Saudi Arabia 2014 through 2022. We employed broad range phylogenetic phylodynamic methods understand predominant epidemiological time scales, determinants, their dynamics. also integrated data detailed electronic health record (EHR) for clinical implications different strains. discovered diverse population underlying infections, most belonging Clonal Complex 14 (CC14) exhibiting dominance. Specifically, observed emergence continuous expansion dominant ST2096 CC14 clade across wards recent years. These acquired mutations against colistin extended spectrum β-lactamase (ESBL) carbapenemase genes, namely blaOXA-48 blaOXA-232, located three distinct plasmids, scales. Strains exhibited high level presence siderophore aerobactin (iuc) locus situated same mosaic plasmid ESBL gene. Integration EHR confirmed significant link between colonization by diagnosis sepsis elevated in-hospital mortality (p-value < 0.05). Overall, these results demonstrate significance illustrate rapid MDR setting.

Language: Английский

Citations

5
The F-pilus biomechanical adaptability accelerates conjugative dissemination of antimicrobial resistance and biofilm formation DOI Creative Commons
Jonasz B. Patkowski, Tobias Dahlberg, Himani Amin

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: April 5, 2023

Abstract Conjugation is used by bacteria to propagate antimicrobial resistance (AMR) in the environment. Central this process are widespread conjugative F-pili that establish connection between donor and recipient cells, thereby facilitating spread of IncF plasmids among enteropathogenic bacteria. Here, we show F-pilus highly flexible but robust at same time, properties increase its thermochemical mechanical stresses. By a combination biophysical molecular dynamics methods, presence phosphatidylglycerol molecules contributes structural stability polymer. Moreover, important for successful delivery DNA during conjugation facilitates rapid formation biofilms harsh environmental conditions. Thus, our work highlights importance adaptations efficient AMR genes bacterial population protect against action antibiotics.

Language: Английский

Citations

13
Whole Genome Characterization of the High-Risk Clone ST383 Klebsiella pneumoniae with a Simultaneous Carriage of blaCTX-M-14 on IncL/M Plasmid and blaCTX-M-15 on Convergent IncHI1B/IncFIB Plasmid from Egypt DOI Creative Commons
Eva A. Edward, Nelly M. Mohamed, Azza S. Zakaria

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(6), P. 1097 - 1097

Published: May 26, 2022

Recently, Egypt has witnessed the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae, which posed a serious healthcare challenge. The accelerated dissemination blaCTX-M genes among these MDR K. particularly blaCTX-M-14 and blaCTX-M-15, have been noted. In this study, we investigated occurrence blaCTX-M-IV pneumoniae recovered from laboratory major hospital in Alexandria. 23 tested isolates showed an phenotype gene was detected ≈22% isolates. transformation plasmids harboring to chemically competent cells Escherichia coli DH5α successful three out five blaCTX-M-IV-positive Whole genome sequencing K22 indicated that isolate belonged high-risk clone ST383, showing simultaneous carriage on IncL/M plasmid, i.e., pEGY22_CTX-M-14, blaCTX-M-15 hybrid IncHI1B/IncFIB pEGY22_CTX-M-15. Alignment both revealed high similarity with those originating UK, Germany, Australia, Russia, China, Saudi Arabia, Morocco. pEGY22_CTX-M-15 mosaic plasmid demonstrated convergence virulence genes. such enhanced genetic plasticity constitutes perfect path for evolution causing invasive untreatable infections especially country burden infectious diseases as Egypt. Therefore there is imperative need countrywide surveillances monitor prevalence superbugs limited therapeutic options.

Language: Английский

Citations

18
Detection and characterization of putative hypervirulent Klebsiella pneumoniae isolates in microbiological diagnostics DOI Creative Commons
Bernd Neumann,

Claudia Stürhof,

Anca Rath

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 3, 2023

Abstract Hypervirulent Klebsiella pneumoniae strains (hvKp) can cause invasive community-acquired infections in healthy patients of all ages. In this study, the prevalence putative hvKp a German tertiary center was investigated and were characterized by phenotypic molecular assays. All K. isolates routine microbiological diagnostics from single screened string-testing over period 6 months. String-test positive (≥ 0.5 mm) re-evaluated on different media under various conditions (aerobe, anaerobe). For string-test isolates, genes (magA, iutA, rmpA rmpA2) associated with hypermucoviscosity hypervirulence amplified multiplex PCR. PCR-positive subjected to whole-genome sequencing sedimentation biofilm formation From 1310 clinical 100 (7.6%) string test positive. these, 9% (n = 9) defined as (string-test+/PCR+). Highest rate string-test-positive observed MacConkey agar aerobic conditions. Amongst these nine international lineage ST23 carrying hvKp-plasmid pKpVP-1 most common, but also rare ST86 pKpVP-2 identified. showed weak formation. conclusion, string-positive, respectively 0.69% hypervirulent. best medium for screening.

Language: Английский

Citations

11
Genomic Insights into CRISPR-Harboring Plasmids in the Klebsiella Genus: Distribution, Backbone Structures, Antibiotic Resistance, and Virulence Determinant Profiles DOI
Jinzhao Long, Jiangfeng Zhang,

Yanyan Xi

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 15, 2023

CRISPR systems are often encoded by many prokaryotes as adaptive defense against mobile genetic elements (MGEs), but several MGEs also recruit components to perform additional biological functions. Type IV-A identified in Klebsiella plasmids, yet the distribution, characterization, and role of these plasmids carrying whole genus remain unclear. Here, we performed large-scale comparative analysis using publicly available plasmid genomes.

Language: Английский

Citations

10