Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates DOI Creative Commons
Andrey Shelenkov,

Yulia Mikhaylova,

Shushanik Voskanyan

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1314 - 1314

Published: May 17, 2023

Klebsiella pneumoniae, a member of the Enterobacteriaceae family, has become dangerous pathogen accountable for large fraction various infectious diseases in both clinical and community settings. In general, K. pneumoniae population been divided into so-called classical (cKp) hypervirulent (hvKp) lineages. The former, usually developing hospitals, can rapidly acquire resistance to wide spectrum antimicrobial drugs, while latter is associated with more aggressive but less resistant infections, mostly healthy humans. However, growing number reports last decade have confirmed convergence these two distinct lineages superpathogen clones possessing properties both, thus imposing significant threat public health worldwide. This process horizontal gene transfer, which plasmid conjugation plays very important role. Therefore, investigation structures ways plasmids spread within between bacterial species will provide benefits prevention measures against powerful pathogens. this work, we investigated multidrug-resistant isolates using long- short-read whole-genome sequencing, allowed us reveal fusion IncHI1B/IncFIB ST512 capable simultaneously carrying hypervirulence (iucABCD, iutA, prmpA, peg-344) determinants (armA, blaNDM-1 others), obtain insights their formation transmission mechanisms. Comprehensive phenotypic, genotypic phylogenetic analysis isolates, as well repertoire, was performed. data obtained facilitate epidemiological surveillance high-risk development strategies them.

Language: Английский

Clinical and Genomic Evolution of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infections over Two Time Periods at a Tertiary Care Hospital in South India: A Prospective Cohort Study DOI Creative Commons
Abi Manesh, Chaitra Shankar, Mithun Mohan George

et al.

Infectious Diseases and Therapy, Journal Year: 2023, Volume and Issue: 12(5), P. 1319 - 1335

Published: April 16, 2023

The objective of this study was to examine the evolution carbapenem-resistant Klebsiella pneumoniae (CRKp) infections and their impact at a tertiary care hospital in South India.A comparative analysis clinical data from two prospective cohorts patients with CRKp bacteremia (C1, 2014-2015; C2, 2021-2022) carried out. Antimicrobial susceptibilities whole genome sequencing (WGS) selected isolates were also analyzed.A total 181 enrolled study, 56 C1 125 C2. shifted critically ill neutropenia others (ICU stay: C1, 73%; 54%; p = 0.02). overall mortality rate 50% introduction ceftazidime-avibactam did not change significantly (54% versus 48%; 0.49). Oxacillinases (OXA) 232 most common mechanisms resistance. WGS showed New Delhi metallo-β-lactamase-5 (NDM-5), higher genetic diversity, accessory content, plasmid burden, as well increased convergence hypervirulence carbapenem resistance C2.CRKp continues pose significant threat, despite new antibiotics. highlights virulence pathogen on patient outcomes India, providing valuable information for clinicians researchers.

Language: Английский

Citations

10
Co-existence of two blaNDM-5 and blaOXA-181 on distinct plasmids in a carbapenem-resistant Klebsiella pneumoniae from a tertiary hospital, Tanzania DOI Creative Commons

Lawrence Mapunda,

Anthon Mwingwa,

Doreen Kamori

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

To understand the mechanisms of carbapenem-resistant Klebsiella pneumoniae (CRKP) from Tanzania and characterize genomes carrying carbapenemase genes.

Language: Английский

Citations

0
Systematic review and meta-analysis on the carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates DOI Creative Commons

Motahareh Sabaghi Qala Nou,

Zahra Amirian, Fatemeh Dehghani

et al.

BMC Pharmacology and Toxicology, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 30, 2025

The global dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) poses a critical threat to public health. However, comprehensive data on the prevalence and resistance rates CR-hvKp are limited. This systematic review meta-analysis aim estimate pooled carbapenem among hvKp strains assess distribution carbapenemase genes. A search ISI Web Science, PubMed, Google Scholar was conducted identify studies reporting in strains. genes calculated using event with 95% confidence intervals. total 36 encompassing 1,098 were included. 49% for imipenem, 53.2% meropenem, 38.2% ertapenem. Carbapenemase gene 19.1% blaVIM, 22.0% blaNDM, 43.4% blaOXA-48, 58.8% blaKPC. high widespread underscore their significant These findings highlight urgent need enhanced surveillance, rapid diagnostic tools, stringent infection control measures mitigate spread CR-hvKp. Future research should focus understanding mechanisms developing targeted therapeutic strategies address this challenge.

Language: Английский

Citations

0
Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades infecciosas y microbiologia clinica (English ed ), Journal Year: 2025, Volume and Issue: 43(2), P. 102 - 109

Published: Feb. 1, 2025

Two main Klebsiella pneumoniae pathotypes are of public health concern, classical K. (cKP), with high antibiotic resistance acquisition capacity, and hypervirulent (hvKP). The emergence antibiotic-resistant pneumoniae, especially carbapenem resistance, is worrisome require effective methods for detection treatment. Different evolutionary paths contribute to the hypervirulence commonly via plasmids by hvKP (CR-hvKP) or virulence CRKp (hv-CRKp). ST11-KL64 together blaKPC-2, most extended hv-CRKP lineage acquiring associated biomarkers, rmpA, rmpa2, iroBCDEN, iucABCDiutA, peg344. In addition ST11, other clones have been reported in Europe such as ST101, ST147 ST512, highlighting association OXA-48 NDM carbapenemases. Although still very rare Spain, cases increasing recent years, mainly due ST23-K1, ST380-K2 ST86-K2. Management infections requires active therapy based primarily on susceptibility patters site infection.

Language: Английский

Citations

0
Plasmidome analyses of Klebsiella pneumoniae coproducing blaKPC-2 and blaNDM-1 in Southern Brazil: Characterization of Mobile Genetic Elements DOI Creative Commons

M K H Berdichevski,

Ricardo Romão Guerra, Daniella Cristina Rodrigues Pereira

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Infections due to carbapenemase-producing Enterobacterales harboring more than one carbapenemase-encoding gene spreads mainly by plasmid and transposon mobilization. Analyze the mobile genetic elements carrying blaKPC blaNDM of K. pneumoniae carbapenemase co-producers (KpKN). isolates with reduced susceptibility carbapenems were obtained from 2016 2023. To evaluate environment KpKN, 22 selected for antimicrobial testing whole-genome sequencing (WGS). The blaKPC-2 was carried IncN/IncFIB, a novel co-integrated in Tn4401b transposon. blaNDM-1 disseminated only two KpKN recovered before 2020 IncHI1B/IncFIB type, Tn3000 Noteworthy, since showed IncA/C an IS26-flanked pseudo-composite containing ISCR1, which also had genes that confer resistance sulfonamides, aminoglycosides, macrolides, quinolones, amphenicols, tetracyclines, rifampicin, sulfonamide, trimethoprim. belong ST11 ST16. changes during different periods could be related higher dissemination large number present may have increased co-selection this through wide use antimicrobials pandemic.

Language: Английский

Citations

0
New insights and perspectives on the virulence of hypervirulent Klebsiella pneumoniae DOI
Ifeanyi Elibe,

Toluwalase Odunayo,

Chibuzor Kenneth Uwazie

et al.

Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Language: Английский

Citations

0
The Klebsiella pneumoniae tellurium resistance gene terC contributes to both tellurite and zinc resistance DOI Creative Commons

Ruixiang Yang,

Shuang Han,

Yanshuang Yu

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

ABSTRACT Klebsiella pneumoniae is widely recognized as a pathogen responsible for hospital-acquired infections and community-acquired invasive infections. It has rapidly become significant global public health threat due to the emergence of hypervirulent multidrug-resistant strains, which have increased challenges associated with treating life-threatening Tellurium resistance genes are widespread on virulence plasmids in K. isolates. However, core function ter operon ( terZABCDEF ) remains unclear. In this study, P1927 strain was isolated from sputum hospitalized pneumonia patient. The operon, along antimicrobial genes, identified large hybrid plasmid P1927. We generated terC deletion mutant demonstrated that exhibited reduced Galleria mellonella larva infection model. Further physiological functional analysis revealed not only important Te(IV) but also Zn(II), Mn(II), phage infection. All were highly inducible by stronger inducer than Te(IV), terBCDE induced Mn(II). Collectively, our study demonstrates novel functions TerC Zn(II) . IMPORTANCE health. Although clinical isolates, its been proposed proteins encoded form multi-site metal-binding complex, exact still unknown. TerC, central component tellurium determinant, previously shown interact outer membrane OmpA KpsD Escherichia coli , suggesting potential changes structure properties. Here, we report confers infection, be strong operon. Furthermore, factor. Taken together, results expand understanding role providing deeper insights into link between heavy metal(loid) determinants pathogenic bacteria.

Language: Английский

Citations

0
Comprehensive Genomic Analysis of Klebsiella pneumoniae and Its Temperate N-15-like Phage: From Isolation to Functional Annotation DOI Creative Commons
Reham Yahya,

Aljawharah Albaqami,

Amal Alzahrani

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 908 - 908

Published: April 15, 2025

Antibiotic resistance to Klebsiella pneumoniae poses a major public health threat, particularly in intensive care unit (ICU) settings. The emergence of extensively drug-resistant (XDR) strains complicates treatment options, requiring deeper understanding their genetic makeup and potential therapeutic targets. This research delineated an strain obtained from ICU patient telomeric temperate phage derived hospital effluent. bacteria showed strong multiple antibiotics, including penicillin (≥16 μg/mL), ceftriaxone (≥32 meropenem (≥8 which was caused by SHV-11 beta-lactamase, NDM-1 carbapenemase, porin mutations (OmpK37, MdtQ). categorized as K46 O2a types carried virulence genes involved iron acquisition, adhesion, immune evasion, well plasmids (IncHI1B_1_pNDM-MAR, IncFIB) eleven prophage regions, reflecting its adaptability dissemination. 172,025 bp linear genome 46.3% GC content the N-15-like genomic similarities phages Sugarlandvirus genus, especially those that infect K. pneumoniae. There were structural proteins (11.8%), DNA replication repair enzymes (9.3%), toxin–antitoxin system (0.4%) encoded genome. A protelomerase ParA/B partitioning indicate is replicating maintaining itself manner similar N15 phage, renowned for plasmid throughout lysogeny. Understanding dynamics antibiotic pathogen development requires knowledge like this one, are known nature function altering bacterial profiles. regulatory also provide model into biology effects on microbial communities. importance genomes larger framework ecology evolution emphasized research.

Language: Английский

Citations

0
Mapping Antimicrobial Resistance in Escherichia coli and Klebsiella pneumoniae from Complicated Urinary Tract Infections in Oman: Phenotypic and Genotypic Insights DOI Creative Commons

Nawal AL Shizawi,

Zaaima AL-Jabri, Fatima Khan

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(9), P. 1062 - 1062

Published: April 22, 2025

Background: Mapping the local etiology and susceptibility of common pathogens causing complicated urinary tract infection (cUTI) is important for promoting evidence-based antimicrobial prescribing. Evaluating prevalence extended-spectrum beta-lactamase (ESBL), AmpC (AmpC), carbapenemase-producing Enterobacterales (CPEs) equally as it informs treatment guidelines empiric management. Whole genome sequencing (WGS) enhances resistance (AMR) surveillance by complementing phenotypic testing, offering deeper insights into mechanisms, transmissions, evolutions. Integrating routine AMR monitoring can significantly improve global efforts to combat resistance. Methods: Antimicrobial profiles isolates from cUTI were collected patients presenting with Sultan Qaboos University Hospital, Muscat Suhar Suhar, Oman. Automated systems well manual methods used detection ESBL, AmpC, CPE. ESBLs, β-lactamases, CPEs further detected methods: double-disk synergy test ESBL; disk approximation assay D69C set mCIM KPC/IMP/NDM/VIM/OXA-48 Combo kit WGS was carried out in 11 FOX-resistant E. coli (22 carbapenem-resistant K. pneumoniae) varying susceptibilities identify circulating clades, genes, plasmids. Bioinformatic analysis performed using online tools. Results: The patterns follows: nitrofurantoin (96%), fosfomycin (100%), fluoroquinolones (44%), aminoglycosides (93%), piperacillin-tazobactam (95%), carbapenems (98%). In comparison, rates pneumoniae far lower: (38%), (89%), (82%), (72%), (83%). pneumoniae, however, more susceptible at 47% comparison coli. ESBL among 37.2% CRE 6.2% while estimated 5.4%. It observed that predominant producer, major (CREs) producer. No multi-locus sequence typing (MLST) lineage AmpC-producing nine MLST lineages being identified eleven isolates: ST-10, ST-69, ST-77, ST-131, ST-156, ST-167, ST-361, ST-1125, ST-2520. On other hand, a less diverse spectrum (ST-2096, ST-231, ST-147, ST-1770, ST-111) pneumoniae. Among five lineages, ST-2096 (twelve isolates) ST-147 (seven predominated. revealed DHA-1 plasmid-mediated gene coli, OXA-232 NDM-5 most carbapenemase genes All DHA-1-positive co-harbored quinolone qnrB4 sulfonamide sul1 no aminoglycoside detected. majority CPE β-lactamase such blaCTX-M-15, blaSHV, blaTEM; all OqxAB; 77% armA. Conclusions: Our results suggest an excellent choice treating cystitis caused both appropriate but not Aminoglycosides are intravenous alternatives spare carbapenems. carbapenemases predominated, suggesting significant flux lack stable clades this region. contrast, predominated Klebsiella circulation these profiling provides understanding genetic basis offers comprehensive pathogen evolution transmission patterns.

Language: Английский

Citations

0
Genome Characterization of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae Strains, Carrying Hybrid Resistance-Virulence IncHI1B/FIB Plasmids, Isolated from an Egyptian Pediatric ICU DOI Creative Commons

Heba Ali Hammad,

Radwa Abdelwahab,

Douglas F. Browning

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1058 - 1058

Published: May 1, 2025

Despite the increased reporting of Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) in Egypt, there is a paucity information regarding molecular characteristics such strains. Herein, we present genome sequence two CR-hvKp strains, K22 and K45, which were isolated from VAP (ventilator-associated-pneumonia) patients admitted to pediatric ICU at Assiut University Children’s Hospital, Egypt. K45 isolates subjected antimicrobial susceptibility testing whole-genome sequencing. Genomic analysis was performed characterize each strain, determining their plasmids, resistance (AMR) genes, virulence determinants. possessed an extensive drug phenotype (XDR), whilst exhibited multidrug (MDR), with sequencing revealing presence diverse array AMR genes. Both strains resistant carbapenem antibiotic imipenem, carrying OXA-48 carbapenemase, additionally possessing NDM-1 carbapenemase. Each strain considered high-risk, respectively belonging types ST383 ST14 genes implicated hypervirulence (e.g., iucABCD-iutA rmpA). Importantly, both carried multiple plasmid replicons, including AMR/virulence IncHI1B/FIB hybrid MDR IncL/M plasmids. This report highlights critical role plasmids evolution virulent K. suggests circulation plasmid, simultaneously disseminating hypervirulence, amongst within Hospital.

Language: Английский

Citations

0