Pelvic infection caused by periodontitis: A case report DOI Creative Commons
Cuihong Tian,

Hongle Yan,

Ping Tang

et al.

Case Reports in Women s Health, Journal Year: 2023, Volume and Issue: 40, P. e00563 - e00563

Published: Nov. 10, 2023

Oral bacteria in patients with periodontitis can disseminate into the bloodstream via broken oral epithelial cells, causing odontogenic maxillofacial infections, brain abscesses and endocarditis. However, pelvic infection caused by is rare. The case of a 48-year-old woman long history recurrent periodontal who complained abdominal distention pain for 14 days after dental implantation, reported here. Pelvic ultrasound magnetic resonance imaging signaled multiple inflammatory encapsulated effusions posterior uterus, which were removed laparoscopic surgery tested metagenomic next-generation sequencing (mNGS). Through mNGS, numerous pathogens, including Filifactor alocis, identified effusions. patient was subsequently diagnosed originating from periodontitis, recovered undergoing targeted antibacterial treatment. Thus, possibility extrabuccal complications or invasive procedures merits closer attention.

Language: Английский

Analysis of metagenomic data DOI
Shaopeng Liu, Judith Rodríguez, Viorel Munteanu

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
Application of metagenomic next-generation sequencing in treatment guidance for deep neck space abscess DOI Creative Commons
Han Lei,

Jiarui Liao,

Yu Lin

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 26, 2025

Infectious etiologies of deep neck space abscess (DNSA) by conventional culture tests can be challenging, which also leads to frequent irrational antibiotic usage. Metagenomic next-generation sequencing (mNGS), as a novel method for analyzing the complex microbial ecosystem from clinical samples, has been utilized in research and practice various infectious diseases but abscess. We here aimed explore value mNGS pathogen detection treatment guidance DNSA patients compared with tests. One hundred six diagnosed were retrospectively enrolled allocated into group according whether was conducted. The effectiveness culture. Effectiveness mNGS-modified antimicrobial therapy evaluated comparing outcomes between two groups. showed significantly higher rate than (p < 0.05) faster result acquisition. Treatment success (RR: 1.22, 95%CI: 1.07–1.82, p = 0.033). Besides, had shorter duration therapy, hospital stay less medical costs 0.05). is an effective technology facilitating improving patients.

Language: Английский

Citations

0
Clinical implement of Probe‐Capture Metagenomics in sepsis patients: A multicentre and prospective study DOI Creative Commons
Qin Sun, Ran Teng, Qiankun Shi

et al.

Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

Language: Английский

Citations

0
Metagenomic next-generation sequencing of plasma cell-free DNA improves the early diagnosis of suspected infections DOI Creative Commons
Hui Zhang,

Ruobing Liang,

Yunzhu Zhu

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 12, 2024

Metagenomic next-generation sequencing (mNGS) could improve the diagnosed efficiency of pathogens in bloodstream infections or sepsis. Little is known about clinical impact mNGS test when used for early diagnosis suspected infections. Herein, our main objective was to assess efficacy utilizing blood samples perform infections, as well evaluate its potential guiding antimicrobial therapy decisions.

Language: Английский

Citations

3
2030: will we still need our microbiologist? DOI Open Access
Inès Lakbar, Mervyn Singer, Marc Léone

et al.

Intensive Care Medicine, Journal Year: 2023, Volume and Issue: 49(10), P. 1232 - 1234

Published: Aug. 22, 2023

Language: Английский

Citations

8
The clinical importance of metagenomic next-generation sequencing in detecting disease-causing microorganisms in cases of sepsis acquired in the community or hospital setting DOI Creative Commons
Dan Zhang, Xingxing Li, Yu Wang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 15, 2024

Although metagenomic next-generation sequencing (mNGS) is commonly used for diagnosing infectious diseases, clinicians face limited options due to the high costs that are not covered by basic medical insurance. The goal of this research challenge bias through a thorough examination and evaluation clinical importance mNGS in precisely identifying pathogenic microorganisms cases sepsis acquired community or hospitals. A retrospective observational study took place at tertiary teaching hospital China from January December 2021. Data on 308 patients were collected, performance etiological was compared between traditional culture method. Two hundred twenty-nine observed community-acquired (CAS) group 79 hospital-acquired (HAS) group. In comparison with conventional culture, showed significantly higher rate positivity both CAS (88.21% vs. 25.76%, adj.P < 0.001) HAS (87.34% 44.30%, 0.001), particularly across various infection sites specimens, which influenced factors like antibiotic exposure timing frequency technology. Sepsis pathogens detected broad, especially viruses, Mycobacterium tuberculosis, atypical pathogens, mixed being common, bacterial-viral co-detection. Based optimization antimicrobial therapy using mNGS, 58 underwent de-escalation, two switched antiviral therapy, 14 initiated treatment resulting reduction overuse but without significant impact prognosis. exhibited critical condition, poor prognosis, expenses, variations etiology, yet results did result increased either demonstrates efficacy multiple responsible sepsis, bacteria viruses prevalent. Variability microbiological profiles among different setting underscores vigilance. Therefore, adoption diagnosis warrants acknowledgment promotion.

Language: Английский

Citations

2
Blood plasma metagenomic next-generation sequencing for identifying pathogens of febrile neutropenia in acute leukemia patients DOI Creative Commons
Yan Qi,

Wuqiang Lin,

Bin Liao

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 20, 2023

To investigate the value of metagenomic next-generation sequencing (mNGS) in acute leukemia (AL) patients with febrile neutropenia (FN). We retrospectively reviewed 37 AL FN and compared results mNGS blood culture (BC) clinical features mNGS-positive group mNGS-negative group. A total 14 detected pathogens were final diagnosis, which 9 strains only by 5 both BC. The top Klebsiella pneumoniae, Pseudomonas aeruginosa Stenotrophomonas maltophilia. 67.57% (25/37) bacterial infections, 2.7% (1/37) fungal or viral infections. diagnostic positivity rate (25/37, 67.6%) was significantly higher than that BC (7/37, 18.9%), difference statistically significant (p < 0.05). Then, we explored distinction between group, 3 filtered, including lymphocyte count (LY), creatinine levels (Cr), white cell (WBC). Our study demonstrated early implementation can effectively improve efficacy pathogen detection FN. ability to detect additional made a valuable tool management infectious complications this patient population. Furthermore, identified associated provided insights for indication infection

Language: Английский

Citations

6
Pathogen Diagnosis Value of Nanopore Sequencing in Severe Hospital-Acquired Pneumonia Patients DOI Creative Commons
Xin Zhao,

Yue Ge,

Yuan Zhang

et al.

Infection and Drug Resistance, Journal Year: 2023, Volume and Issue: Volume 16, P. 3293 - 3303

Published: May 1, 2023

Background: Next-generation sequencing of the metagenome (mNGS) is increasingly used in pathogen diagnosis for infectious diseases due to its short detection time. The time Oxford Nanopore Technologies (ONT) sequencing-based etiology further shortened compared with that mNGS, but only a few studies have verified advantage and accuracy ONT diagnosis. In 2022, study confirmed there was no significant difference sensitivity specificity between mNGS suspected community-acquired pneumonia patients, which clinical patients SHAP. Methods: From October 24 November 20, 10 severe hospital-acquired (SHAP) Nanfang Hospital intensive care unit (ICU) were prospectively enrolled. Bronchoalveolar lavage fluid (BALF) collected sequencing, traditional culture. differences diagnostic agreement among culture method, composite compared. Results: Compared had (9.6± 0.7 h versus 24.7± 2.7 132± 58 h, P < 0.05). rate 73.3% (kappa value=0.737, Conclusion: has potential rapidly identifying pathogens. Keywords: Technologies, metagenomic next-generation pneumonia,

Language: Английский

Citations

5
Uncovering ferroptosis in Parkinson’s disease via bioinformatics and machine learning, and reversed deducing potential therapeutic natural products DOI Creative Commons
Peng Wang, Qi Chen,

Zhuqian Tang

et al.

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: July 6, 2023

Objective: Ferroptosis, a novel form of cell death, is closely associated with excessive iron accumulated within the substantia nigra in Parkinson's disease (PD). Despite extensive research, underlying molecular mechanisms driving ferroptosis PD remain elusive. Here, we employed bioinformatics and machine learning approach to predict genes investigate interactions between natural products their active ingredients these genes. Methods: We comprehensively analyzed differentially expressed (DEGs) for (PDFerDEGs) by pairing 3 datasets (GSE7621, GSE20146, GSE202665) from NCBI GEO database FerrDb V2 database. A was then used screen PDFerDEGs signature mined interacted product components based on screened Finally, mapped network combined genes, carried out docking validation core targets uncover potential therapeutic PD. Results: identified 109 that were significantly enriched biological processes KEGG pathways (including ion homeostasis, transport ferroptosis, etc.). obtained 29 overlapping 6 hub (TLR4, IL6, ADIPOQ, PTGS2, ATG7, FADS2) screening two algorithms. Based this, 263 subsequently "Overlapping Genes-Ingredients" network. According network, top 5 (quercetin, 17-beta-estradiol, glycerin, trans-resveratrol, tocopherol) molecularly docked reveal role treatment Conclusion: Our findings suggested are play progression Taken together, bind well FADS2), highlighting biomarkers

Language: Английский

Citations

5
Etiological stratification and prognostic assessment of haemophagocytic lymphohistiocytosis by machine learning on onco-mNGS data and clinical data DOI Creative Commons
Lin Wu, Xuefang Cao,

Jingshi Wang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Hemophagocytic lymphohistiocytosis (HLH) is a rare, complicated and life threatening hyperinflammatory syndrome that maybe triggered by various infectious agents, malignancies rheumatologic disorders. Early diagnosis identification of the cause essential to initiate appropriate treatment improve quality survival patients. The recently developed Onco-mNGS technology can be successfully used for simultaneous detection infections tumors.

Language: Английский

Citations

1