Green synthesis and characterization of silver nanoparticles from Ducrosia flabellifolia Boiss. aqueous extract: Anti-quorum sensing screening and antimicrobial activities against ESKAPE pathogens

Cellular and Molecular Biology, Journal Year: 2024, Volume and Issue: 70(2), P. 88 - 96

Published: Feb. 29, 2024

Language: Английский

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Decontamination of Fused-Silica Surfaces by UVC Irradiation as Potential Application on Touchscreens

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 2099 - 2099

Published: Oct. 21, 2024

The contamination of surfaces by antibiotic-resistant pathogens presents an escalating challenge, especially on touchscreens in public settings such as hospitals, airports, and means transport. Traditional chemical cleaning agents are often ineffective leave behind harmful residues. Thus, the application optical radiation is gaining relevance a rapid, effective, environmentally friendly disinfection method. This study examines publicly accessible efficacy irradiation approach for microorganisms quartz with UVC LEDs. In this setup, LED laterally coupled into plate that serves cover glass simplified touchscreen model. process allows surface, without user being exposed to hazardous radiation. To assess process, range bacteria, mostly ESKAPE surrogates,

Language: Английский

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Antimicrobial peptides: An alternative strategy to combat antimicrobial resistance

Drug Discovery Today, Journal Year: 2025, Volume and Issue: unknown, P. 104305 - 104305

Published: Feb. 1, 2025

Language: Английский

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Silyl-Lipid Functionalized N-Acyl Homoserine Lactones as Modulators of Bacterial Cell–Cell Communication

ACS Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

We report silyl-lipid derivatives of N-acyl l-homoserine lactones (AHLs) that have nanomolar activities in LuxR-type quorum sensing receptors Gram-negative bacterial pathogens. A collection AHLs were designed and synthesized to represent three general structural classes based on native AHL signals synthetic receptor modulators. The routes feature straightforward hydrosilylation aryl silylation reactions access groups are not readily accessible analogous all-carbon chemistry. Of the 17 compounds evaluated, eight identified with either agonistic or submicromolar antagonistic LasR from common pathogen Pseudomonas aeruginosa using E. coli reporter gene assays. Several agonists retained high a P. system also active another related receptor, EsaR Pantoea stewartii. Light scattering computational experiments indicate group can alter aggregation capabilities lipophilicities relative tails, engendering larger aggregate formation water higher average. These properties, along their strong activity profiles receptors, suggest could provide value as chemical probes study mechanisms bacteria roles signal lipophilicity this communication process.

Language: Английский

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A Real-Life Study of Prolonged Meropenem Infusion in Neonates and Children Admitted to Intensive Care Units: Are Three Hours Long Enough?

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(5), P. 1488 - 1488

Published: Feb. 23, 2025

Background/Objectives: Meropenem is a broad-spectrum antibiotic essential for treating resistant Gram-negative infections in pediatric patients. Current dosing recommendations may not consistently achieve optimal pharmacokinetic (PK) targets, especially critically ill children. Methods: We conducted retrospective cohort study at IRCCS Istituto Giannina Gaslini, analyzing 97 plasma levels from 86 patients (<18 years) hospitalized between January 2020 and December 2023 the neonatal intensive care unit. Patients receiving meropenem proven or suspected were included. Demographic, clinical, PK parameters assessed, with focus on trough concentrations (Ctrough). Results: The median age was 25 months, neonates representing 15.5% of cases. Ctrough 2.8 mg/L significantly higher (8.9 mg/L) compared to older (2.2 mg/L, p < 0.001). Only 27.8% achieved target >8 estimated glomerular filtration rate (eGFR) being primary factor influencing these levels. > 8 had lower eGFR (61 mL/min/1.73 m2) those below this threshold (131 m2, = Conclusions: current regimen reliably meet targets patients, particularly augmented renal clearance when pathogens increased MIC. Our findings suggest that dosages prolonged infusion times be necessary optimize therapeutic efficacy against bacteria vulnerable population. Further studies are needed refine strategies improve patient outcomes.

Language: Английский

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Use of essential oils and their components and formulations against multidrug resistant bacteria

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 313 - 357

Published: Jan. 1, 2025

Language: Английский

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Characterization of Novel Phages KPAФ1, KP149Ф1, and KP149Ф2 for lytic efficiency against Clinical MDR Klebsiella pneumoniae Infections

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107440 - 107440

Published: Feb. 1, 2025

Language: Английский

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Machine Learning‐Assisted Prediction and Generation of Antimicrobial Peptides

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Antimicrobial peptides (AMPs) offer a highly potent alternative solution due to their broad‐spectrum activity and minimum resistance development against the rapidly evolving antibiotic‐resistant pathogens. Herein, accelerate discovery process of new AMPs, predictive generative algorithm is build, which constructs peptide sequences, scores antimicrobial using machine learning (ML) model, identifies amino acid motifs, assembles high‐ranking motifs into sequences. The eXtreme Gradient Boosting model achieves an accuracy ≈87% in distinguishing between AMPs non‐AMPs. generated sequences are experimentally validated bacterial pathogens, ≈60% achieved. To refine algorithm, physicochemical features analyzed, particularly charge hydrophobicity peptides. with specific range then removed, lead substantial increase experimental accuracy, from ≈80%. Furthermore, active different fungal strains minimal off‐target toxicity. In summary, silico models for functional motif AMP powerful tools engineering effective combat multidrug resistant

Language: Английский

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Newly isolated terpenoids (covering 2019–2024) from Aspergillus species and their potential for the discovery of novel antimicrobials

Natural Products and Bioprospecting, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 18, 2025

Abstract The rapid emergence of drug-resistant microbial pathogens has posed challenges to global health in the twenty-first century. This development significantly made most antibiotics ineffective treatment infections they cause, resulting increasing costs and annual death rates. To address challenge by these pathogens, we explore potential secondary metabolites from Aspergillus species as a source new effective therapeutic agents treat infections. Terpenoids, distinct group natural products, are extensively distributed plants fungi, have been attributed with significant antibacterial, anticancer, antiviral activities. In this review, present an overview species, review novel terpenoids isolated them 2019 April 2024, highlighting anti-infective activity against members ESKAPE pathogens. We further focus on strategies through which structural framework could be modified and/or optimized feed pipeline lead compounds targeting Overall, provides insight into applications sourced for discovery fungi combat antimicrobial resistance. Graphical

Language: Английский

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Multifaceted quorum-sensing inhibiting activity of 3-(Benzo[d][1,3]dioxol-4-yl)oxazolidin-2-one mitigates Pseudomonas aeruginosa virulence

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 19, 2025

As antibiotic resistance escalates into a global health crisis, novel therapeutic approaches against infectious diseases are in urgent need. Pseudomonas aeruginosa, an adaptable opportunistic pathogen, poses substantial challenges treating range of infections. The quorum-sensing (QS) system plays pivotal role orchestrating the production large set virulence factors cell density-dependent manner, and anti-virulence strategy targeting QS may show huge potential. Here, we present comprehensive investigation potential synthesized compound 3-(benzo[d][1,3]dioxol-4-yl)oxazolidin-2-one (OZDO, C10H9NO4) as inhibitor to curb P. aeruginosa. By employing integrated approach encompassing silico screening, vitro vivo functional identification, elucidated multifaceted effects OZDO. Molecular docking predicted that OZDO interfered with three core regulatory proteins aeruginosa system. Notably, exhibited significant inhibition on pyocyanin, rhamnolipid extracellular proteases, biofilm formation, motilities Transcriptomic analysis quantitative real-time PCR displayed down-regulation QS-controlled genes OZDO-treated PAO1, reaffirming QS-inhibition activity In assessments using Caenorhabditis elegans-infection model demonstrated mitigated pathogenicity, particularly hypervirulent strain PA14. Moreover, combination polymyxin B aztreonam presented promising avenue for innovative anti-infective therapy. Our study sheds light agent its significance combating aeruginosa-associated

Language: Английский

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