Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp. DOI Creative Commons
Wattana Pelyuntha, Mingkwan Yingkajorn,

Thamonwan Narkpao

et al.

Cosmetics, Journal Year: 2023, Volume and Issue: 10(6), P. 156 - 156

Published: Nov. 14, 2023

The emergence of multidrug-resistant (MDR) Staphylococcus spp. has resulted in the reduced use antibiotics many skincare cosmetic products. Alternative treatments using natural bioactive compounds and chemical agents can be replaced. However, these have induced negative side effects among users are not environmentally friendly. Phage therapy is an alternative to for treatment specific pathogenic bacteria including spp., without harmful on human skin cells microflora. Phages potentially used direct application phage-based products reduce chance infection caused by In present work, we isolated 17 phages from sewage soil samples. A1 showed highest lytic ability at 50% (B1 profile), covering 13 tested isolates aureus (SA), methicillin-resistant S. (MRSA), capitis (SC), epidermidis (SE). representative ATCC 25923 SC1 2.0 ± 0.1 4.1 0.3 log units a multiplicity (MOI) 104 4.2 0.2 4.4 0.5 MOI 105 after 6 h post-phage treatment. transmission electron microscope revealed that phage was classified order Caudovirales family Myoviridae based its appearance. optimal survival presence 0.125% (v/v) solidant DMH suspension 3 post-treatment. Under serum formulation, titers were 0.46 0.85 storage 4 25 °C, whereas reduction 2.96 also observed 37° 90 days. This study provides strong evidence effectiveness diseases MDR concept this could advantageous and/or cosmeceutical industries searching new ingredients cosmetic/cosmeceutical

Language: Английский

Potenziale der Bakteriophagentherapie in Deutschland: Evidenzlage und klinische Relevanz DOI Creative Commons
Felix Broecker, Christian Willy

Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Citations

0
Phage therapy to treat cystic fibrosis Burkholderia cepacia complex lung infections: perspectives and challenges DOI Creative Commons

Jack S. Canning,

Daniel R. Laucirica, Kak‐Ming Ling

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 18, 2024

complex is a cause of serious lung infections in people with cystic fibrosis, exhibiting extremely high levels antimicrobial resistance. These are difficult to treat and associated morbidity mortality. With notable lack new antibiotic classes currently development, exploring alternative strategies for

Language: Английский

Citations

3
Phage Therapy for Orthopaedic Infections: The First Three Cases from the United Kingdom DOI Creative Commons
Daniela Munteanu, John R. Dunn, Gábor Apjok

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 114 - 114

Published: Jan. 22, 2025

Background: Bacteriophages (phages) are viruses that infect and kill bacteria. The antimicrobial resistance crisis has driven renewed interest in phage therapy, including the use of phages to treat chronic orthopaedic infections. Methods: Here, we present results first three patients treated with therapy United Kingdom. Results: patient was May 2023 received active against Staphylococcus aureus. At nine months follow-up, patient’s wound remained healed, C-reactive protein normal walking independently. second Klebsiella pneumoniae S. aureus; infection unresolved. third epidermidis; at six free infection. Endotoxin considered least partially responsible for mild self-limiting adverse effects two cases. Conclusions: These promising hint potential transform care

Language: Английский

Citations

0
Phage susceptibility determinants of the opportunistic pathogen Staphylococcus epidermidis DOI Creative Commons
Christian Beck, Janes Krusche, Ahmed M. A. Elsherbini

et al.

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 78, P. 102434 - 102434

Published: Feb. 16, 2024

Staphylococcus epidermidis is a common member of the human skin and nose microbiomes frequent cause invasive infections. Transducing phages accomplish horizontal transfer resistance virulence genes by mispackaging mobile-genetic elements, contributing to severe, therapy-refractory S. Lytic on other hand can be interesting candidates for new anti-S. phage therapies. Despite importance phages, we are only beginning unravel interactions. Recent studies shed light diversity, host range, receptor specificities. Modulation cell wall teichoic acids, major structures, along with defense mechanisms, crucial determinants susceptibility different groups.

Language: Английский

Citations

2
Coagulase-Negative Staphylococci phages panorama: genomic diversity and in vitro studies for a therapeutic use DOI Creative Commons
Maria Sequeira Lopes, Maria Daniela Silva, Joana Azeredo

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 290, P. 127944 - 127944

Published: Nov. 9, 2024

Coagulase-negative staphylococci (CoNS) are commensal bacteria of the human skin and mucosal membranes. The incidence nosocomial infections caused by these species is on rise, leading to a potential increase in antibiotic tolerance resistance. Phages emerging as promising alternative combat CoNS infections. Scientists isolating phages infecting with particular interest S. epidermidis. This review compiles analyses for several parameters including source, geographical location, host species, morphological diversity, genomic diversity. Additionally, recent studies have highlighted based range, vitro evaluation performance stability, interaction biofilms. comprehensive analysis enables better understanding steps involved using therapeutic purposes.

Language: Английский

Citations

2
SKIN DYSBIOSIS IN ATOPIC DOGS: IS PHAGE THERAPY AN ALTERNATIVE TO ANTIBIOTICS? DOI Creative Commons

Iva Šumonja,

Tina Kotnik

Slovenian Veterinary Research, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Bacterial overgrowth, also known as dysbiosis, is a common concomitant of canine atopic dermatitis. Microbial diversity decreased and coagulase-positive staphylococci are more abundant in dogs with dermatitis compared to healthy dogs. Antimicrobial therapy restores the skin microbiome; however, this effect can diminish after treatment discontinued. Therapies for dysbiosis have traditionally included antibiotics antiseptic medications. Due increasing microbial resistance antibiotics, era novel antimicrobial agents infections has already begun. Recent research highlights potential new options, which one most promising appears be use bacteriophages. Bacteriophages viruses that infect kill bacteria without having negative effects on human or animal cells. This article provides an update veterinary phage approach bacterial infections, focus The clear clinical therapy, its advantages disadvantages, legal, biological, technical, economic challenges it faces further implementation wider application outlined.

Language: Английский

Citations

1
Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp. DOI Creative Commons
Wattana Pelyuntha, Mingkwan Yingkajorn,

Thamonwan Narkpao

et al.

Cosmetics, Journal Year: 2023, Volume and Issue: 10(6), P. 156 - 156

Published: Nov. 14, 2023

The emergence of multidrug-resistant (MDR) Staphylococcus spp. has resulted in the reduced use antibiotics many skincare cosmetic products. Alternative treatments using natural bioactive compounds and chemical agents can be replaced. However, these have induced negative side effects among users are not environmentally friendly. Phage therapy is an alternative to for treatment specific pathogenic bacteria including spp., without harmful on human skin cells microflora. Phages potentially used direct application phage-based products reduce chance infection caused by In present work, we isolated 17 phages from sewage soil samples. A1 showed highest lytic ability at 50% (B1 profile), covering 13 tested isolates aureus (SA), methicillin-resistant S. (MRSA), capitis (SC), epidermidis (SE). representative ATCC 25923 SC1 2.0 ± 0.1 4.1 0.3 log units a multiplicity (MOI) 104 4.2 0.2 4.4 0.5 MOI 105 after 6 h post-phage treatment. transmission electron microscope revealed that phage was classified order Caudovirales family Myoviridae based its appearance. optimal survival presence 0.125% (v/v) solidant DMH suspension 3 post-treatment. Under serum formulation, titers were 0.46 0.85 storage 4 25 °C, whereas reduction 2.96 also observed 37° 90 days. This study provides strong evidence effectiveness diseases MDR concept this could advantageous and/or cosmeceutical industries searching new ingredients cosmetic/cosmeceutical

Language: Английский

Citations

2