medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 20, 2024
Abstract
Background
Gut
microbiota
alteration
is
implicated
in
the
pathogenesis
of
alcoholic
liver
disease
(ALD)
and
HCC.
No
study
has
characterized
dysbiosis
associated
with
ALD
by
microbial
culturomics,
an
approach
that
certifies
viability
allows
characterization
pathobiont
strain
candidates.
Methods
A
case-control
was
conducted
on
patients
without
HCC
(ALD-NoHCC)
(n=16),
(ALD-HCC)
(n=19),
controls
(n=24).
16S
rRNA
amplicon
sequencing
culturomics
were
used
as
complementary
methods
for
gut
microbiome
profiling.
Results
By
Thomasclavelia
ramosa
most
enriched
detected
all
samples
(100%),
while
it
cultivated
only
a
small
proportion
(20%,
p
<
0.001).
3-groups
linear
discriminant
analysis,
T.
increased
explicitly
ALD-HCC
group
(LDA-score
>
5,
0.05).
Conclusions
ramosa,
identified
16
sequencing,
ALD-HCC.
Alongside
recently
reported
vitro
genotoxicity
this
species
colorectal
cancer,
been
candidate
oncobiont
Highlights
The
signature
explored
cultured
from
included
patients,
but
20%
(p
significantly
alcohol-related
sequencing.
putative
ALD-HCC,
thus
opening
new
avenues
diagnosis
treatment.
Graphical
abstract
Gut Pathogens,
Journal Year:
2025,
Volume and Issue:
17(1)
Published: May 7, 2025
Gut
microbiota
alteration
is
implicated
in
the
pathogenesis
of
alcoholic
liver
disease
(ALD)
and
associated
hepatocellular
carcinoma
(HCC).
No
study
has
characterized
dysbiosis
with
ALD
by
microbial
culturomics,
which
certifies
viability
allows
pathobiont
strain
candidates
to
be
characterized.
A
case-control
(n
=
59)
was
conducted
on
patients
without
HCC
(ALD-NoHCC,
n
16),
(ALD-HCC,
19)
controls
24)
groups.
16
S
rRNA
amplicon
sequencing
culturomics
were
used
as
complementary
methods
for
gut
microbiome
profiling.
Compared
control
group,
Thomasclavelia
ramosa
Gemmiger
formicilis
significantly
increased
ALD-HCC
group
Mediterraneibacter
gnavus
ALD-NoHCC
using
sequencing.
By
T.
detected
all
samples
(100%),
most
enriched
since
cultivated
only
a
small
proportion
(20%,
p
<
0.001).
ramosa,
identified
sequencing,
may
ALD-HCC.
These
results
highlight
potential
role
cancer,
line
its
genotoxic
properties
tumor
growth-promoting
effect
gnotobiotic
mice
recently
reported.
Seminars in Liver Disease,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 10, 2024
The
prevalence
of
metabolic
dysfunction-associated
steatotic
liver
disease
(MASLD)—previously
described
as
nonalcoholic
fatty
disease—continues
to
rise
globally.
Despite
this,
therapeutic
measures
for
MASLD
remain
limited.
Recently,
there
has
been
a
growing
interest
in
the
gut
microbiome's
role
pathogenesis
MASLD.
Understanding
this
relationship
may
allow
administration
therapeutics
that
target
microbiome
and/or
its
function
alleviate
development
or
progression.
This
review
will
discuss
interplay
between
structure
and
relation
MASLD,
assess
diagnostic
yield
microbiome-based
signatures
noninvasive
tool
identify
severity,
examine
current
emerging
therapies
targeting
microbiome–liver
axis.
Metabolism and Target Organ Damage,
Journal Year:
2024,
Volume and Issue:
4(3)
Published: July 29, 2024
Steatotic
liver
disease
(SLD),
including
metabolic
dysfunction-associated
steatotic
(MASLD)
and
alcohol-associated
(ALD),
is
the
primary
cause
of
illness
mortality.
In
particular,
MASLD
affects
more
than
30%
global
population,
while
ALD
accounts
for
5.1%
all
diseases
injuries
worldwide.
The
SLD
spectrum
includes
a
variety
clinical
conditions,
from
mild
fatty
inflammation
to
different
stages
fibrosis.
Additionally,
both
conditions
(MASLD
ALD)
can
be
complicated
by
hepatocellular
carcinoma
(HCC),
around
one-third
patients
also
develop
at
least
one
alcohol‐associated
hepatitis
(AH)
episode.
Both
these
are
associated
with
multiple
extrahepatic
complications,
such
as
cardiovascular
disease,
chronic
kidney
malignancies.
MASLD,
rapid
rise
in
obesity
type
2
diabetes
mellitus
(T2DM)
prevalence
due
Westernized
lifestyles
has
led
an
increase
MASLD.
Thus,
prevention
control
cardiometabolic
risk
factors
(CMRFs)
cornerstone
its
treatment.
Hypertension
atherogenic
dyslipidemia
important
CMRFs
Susceptible
individuals
adversely
affected
even
small
amount
alcohol
consumption
(though
there
no
agreed
definition
amount),
increasing
severe
outcomes
faster
progression
disease.
This
review
explores
that
play
role
development
SLD,
especially
focusing
on
management
levels
use
prevent
progression.
Microbiological Research,
Journal Year:
2024,
Volume and Issue:
288, P. 127870 - 127870
Published: Aug. 10, 2024
Antimicrobial
resistance
(AMR)
is
a
complex
issue
requiring
specific,
multi-sectoral
measures
to
slow
its
spread.
When
people
are
exposed
antimicrobial
agents,
it
can
cause
resistant
bacteria
increase.
This
means
that
the
use,
misuse,
and
excessive
use
of
agents
exert
selective
pressure
on
bacteria,
which
lead
development
"silent"
reservoirs
genes.
These
genes
later
be
mobilized
into
pathogenic
contribute
spread
AMR.
Many
socioeconomic
environmental
factors
influence
transmission
dissemination
genes,
such
as
quality
healthcare
systems,
water
sanitation,
hygiene
infrastructure,
pollution.
The
sporobiota
an
essential
part
gut
microbiota
plays
role
in
maintaining
homeostasis.
However,
because
spores
highly
transmissible
easily,
they
vector
for
resistome,
particularly
mobile
important
tracking,
managing,
limiting
among
commensal
bacterial
species.
FEBS Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 26, 2024
The
human
gut
microbiome
plays
a
crucial
role
in
regulating
intestinal
and
systemic
health,
impacting
host
immune
response
metabolic
function.
Dysbiosis
of
the
is
linked
to
various
diseases,
including
steatotic
liver
diseases.
Metabolic
dysfunction‐associated
disease
(MASLD),
chronic
characterized
by
excess
hepatic
lipid
content
impaired
metabolism,
leading
cause
worldwide.
Among
microbes,
Ruminococcus
gnavus
(
R.
)
has
garnered
attention
for
its
association
with
inflammatory
While
abundance
correlates
fat
accumulation,
further
research
needed
identify
causal
or
therapeutic
intervention
disease.
This
review
surveys
our
current
understanding
development
progression
highlighting
potential
mechanisms
through
metabolite
secretion,
emphasizes
need
comprehensive
analyses
longitudinal
studies
better
understand
gnavus'
impact
on
health.
knowledge
could
pave
way
targeted
interventions
aimed
at
modulating
microbiota
treat
prevent
MASLD
comorbidities.
medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 20, 2024
Abstract
Background
Gut
microbiota
alteration
is
implicated
in
the
pathogenesis
of
alcoholic
liver
disease
(ALD)
and
HCC.
No
study
has
characterized
dysbiosis
associated
with
ALD
by
microbial
culturomics,
an
approach
that
certifies
viability
allows
characterization
pathobiont
strain
candidates.
Methods
A
case-control
was
conducted
on
patients
without
HCC
(ALD-NoHCC)
(n=16),
(ALD-HCC)
(n=19),
controls
(n=24).
16S
rRNA
amplicon
sequencing
culturomics
were
used
as
complementary
methods
for
gut
microbiome
profiling.
Results
By
Thomasclavelia
ramosa
most
enriched
detected
all
samples
(100%),
while
it
cultivated
only
a
small
proportion
(20%,
p
<
0.001).
3-groups
linear
discriminant
analysis,
T.
increased
explicitly
ALD-HCC
group
(LDA-score
>
5,
0.05).
Conclusions
ramosa,
identified
16
sequencing,
ALD-HCC.
Alongside
recently
reported
vitro
genotoxicity
this
species
colorectal
cancer,
been
candidate
oncobiont
Highlights
The
signature
explored
cultured
from
included
patients,
but
20%
(p
significantly
alcohol-related
sequencing.
putative
ALD-HCC,
thus
opening
new
avenues
diagnosis
treatment.
Graphical
abstract
