Synthesis of a di-O-acylated deoxynojirimycin (DNJ) derivative and evaluation of its antibacterial and antibiofilm activity against Staphylococcus aureus and Stenotrophomonas maltophilia

Carbohydrate Research, Journal Year: 2025, Volume and Issue: 550, P. 109379 - 109379

Published: Jan. 11, 2025

Language: Английский

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Role of SMF-1 and Cbl pili in Stenotrophomonas maltophilia biofilm formation

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100253 - 100253

Published: Jan. 1, 2025

Language: Английский

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Integrative in Silico Analysis of Hypothetical Proteins from Stenotrophomonas Maltophilia Nctc10958: Insights into Environmental Adaptation and Virulence Factors

Published: Jan. 1, 2025

Language: Английский

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Exploring Proteases as Alternative Molecular Targets to Tackle Inflammation in Cystic Fibrosis Respiratory Infections

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1871 - 1871

Published: Feb. 21, 2025

Cystic fibrosis (CF) is characterized by chronic respiratory infections and excessive inflammation, driven both host- pathogen-derived proteases. The dysregulated activity of proteolytic enzymes such as neutrophil elastase (NE), cathepsin G, matrix metalloproteases (MMPs) degrades lung tissue, exacerbates airway remodeling, perpetuates inflammatory cycles. Concurrently, bacterial proteases from pathogens Pseudomonas aeruginosa Staphylococcus aureus contribute to immune evasion tissue destruction, compounding disease severity. Despite advances in antimicrobial anti-inflammatory therapies, protease-driven damage remains a critical challenge. This review examines the dual role host CF pathophysiology, highlighting emerging protease-targeted therapies aimed at mitigating inflammation. Strategies explored include inhibition NE, MMPs, proteases, with focus on innovative therapeutic approaches dual-function inhibitors, biologics, advanced drug delivery systems. By restoring protease–antiprotease balance, these interventions offer potential improve clinical outcomes quality life for patients.

Language: Английский

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Stenotrophomonas maltophilia of clinical origin display higher temperature tolerance comparing with environmental isolates

Virulence, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Stenotrophomonas maltophilia is a gram-negative, multidrug-resistant, opportunistic human pathogen responsible for hard-to-treat infections in immunocompromised individuals. Besides being recognized as an important clinical pathogen, S. also widespread the natural environment, with knowledge of pathogenic potential environmental pool still lacking. In this study, we aimed to identify differences virulence-related traits between and isolates by assessing their genotypic phenotypic features. For purpose, 40 from environment 34 obtained patients were analysed. We observed high degree diversity among irrespective origin. Although antibiotic resistance- genes more prevalent isolates, majority analysed present isolates. Most importantly, features, specifically ability form biofilms display twitching motility at body temperature predominantly characteristic Our study indicates that adaptation endure feature strongly linked strains origin, significant when differentiating harmless bacteria

Language: Английский

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Bactericidal effectors of the Stenotrophomonas maltophilia type IV secretion system: functional definition of the nuclease TfdA and structural determination of TfcB

mBio, Journal Year: 2024, Volume and Issue: 15(7)

Published: June 4, 2024

ABSTRACT Stenotrophomonas maltophilia expresses a type IV protein secretion system (T4SS) that promotes contact-dependent killing of other bacteria and does so partly by secreting the effector TfcB. Here, we report structure TfcB, comprising an N-terminal domain similar to catalytic glycosyl hydrolase (GH-19) chitinases C-terminal for recognition translocation T4SS. Utilizing two-hybrid assay measure interactions with T4SS coupling VirD4, documented existence five more substrates. One these was 20845, annotated nuclease. A S. mutant lacking gene 20845 impaired Escherichia coli , Klebsiella pneumoniae Pseudomonas aeruginosa . Moreover, cloned conferred robust toxicity, recombinant E. being rescued when co-expressed its cognate immunity protein. The 899 amino-acid protein, comprised GHH-nuclease in N-terminus, large central region indeterminant function, C-terminus secretion. Engineered variants had mutations predicted site did not impede indicating antibacterial effect involves nuclease activity. Using flow cytometry DNA staining, determined but variants, confers loss content target bacteria. Database searches revealed uncharacterized homologs occur within range These data indicate interbacterial competition through action multiple toxic effectors, including potent, novel DNase. IMPORTANCE is multi-drug-resistant, Gram-negative bacterium emerging pathogen humans. Patients cystic fibrosis are particularly susceptible infection. In hospital water systems various types infections, co-exists bacteria, pathogens such as We previously demonstrated has functional VirB/D4 VI Since most work on system, this observation remains noteworthy. currently stands alone model human expressing biochemical, genetic, cell biological approaches, now both discovery (TfdA) first structural determination bactericidal (TfcB).

Language: Английский

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Correlation between Stenotrophomonas maltophilia incidence and systemic antibiotic use: A 10-year retrospective, observational study in Hungary

European Journal of Microbiology and Immunology, Journal Year: 2024, Volume and Issue: 14(2), P. 185 - 194

Published: March 5, 2024

Extensive use of carbapenems may lead to selection pressure for Stenotrophomonas maltophilia (SM) in hospital environments. The aim our study was assess the possible association between systemic antibiotic and incidence SM. A retrospective, observational carried out a tertiary-care Hungary, January 1st 2010 December 31st 2019. Incidence-density SM resistant trimethoprim-sulfamethoxazole (SXT) standardized 1000 patient-days, while expressed as defined daily doses (DDDs) per 100 patient-days. Mean density infections 0.42/1000 patient-days; 11.08% were SXT, mean SXT-resistant 0.047/1000 Consumption rate colistin, glycopeptides increased by 258.82, 278.94 372.72% from 2019, respectively. Strong significant positive correlations observed with consumption (r: 0.8759; P < 0.001 r: 0.8968; 0.001), SXT 0.7552; = 0.011 0.7004; 0.024), 0.7542; 0.012 0.8138; 0.001) incidence-density/1000 Implementation institutional carbapenem-sparing strategies are critical preserving these life-saving drugs, affect microbial spectrum clinical settings.

Language: Английский

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Characterization and genomic analysis of phage vB_SmaP_c9-N, a novel Stenotrophomonas maltophilia podophage with antibiofilm activity

Archives of Virology, Journal Year: 2024, Volume and Issue: 169(11)

Published: Oct. 21, 2024

Language: Английский

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Evaluation of clinical outcomes and risk factors associated with mortality in patients with Stenotrophomonas maltophilia bloodstream infection: a multicenter study

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 5, 2024

Stenotrophomonas maltophilia, a pathogen that colonizes medical equipment and causes nosocomial infections due to its ability form biofilms, has high mortality rates. This study investigated the risk factors related in patients who were diagnosed with S. maltophilia bacteremia. It is multi-center, retrospective ad observational cohort study. The demographic characteristics, clinical findings, microbiological data, for obtained from records of at ten different hospitals between January 1, 2018, June 30, 2023. included total 321 patients. observed thirty-day rate was 46.1%. A central venous catheter (CVC) present 276 (86%), 66 these (23.9%) CVC removed. While only 18 (5.6%) received appropriate empirical antibiotics, 242 (75.4%) antibiotics according antimicrobial susceptibility test (AST) results treatment revisions. Multivariate analysis revealed advanced age (hazard ratio [HR] = 1.02; 95% confidence interval [CI]: 1.00– 1.03), antibiotic (HR 0.35; CI: 0.23–0.52), removal catheters 0.31; 0.16–0.60) significantly reduced mortality. significant pathogen, reduce rate, switching should be performed as soon possible.

Language: Английский

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Unraveling the complexity of Stenotrophomonas maltophilia – a comprehensive review of current knowledge

Postępy Higieny i Medycyny Doświadczalnej, Journal Year: 2024, Volume and Issue: 78(1), P. 103 - 110

Published: Jan. 1, 2024

Abstract Stenotrophomonas maltophilia is a Gram-negative (G−) opportunistic pathogen, prevalent and concerning for susceptible patients. It primarily causes nosocomial some community-acquired respiratory bloodstream infections. Reservoirs include water, plant rhizospheres, food, animals. Virulence factors motility, biofilm formation, quorum sensing, with ongoing research on these mechanisms. A major concern its multidrug resistance to antimicrobial agents like trimethoprim-sulfamethoxazole (TMP-SMX), β-lactams, macrolides, fluoroquinolones, aminoglycosides, chloramphenicol, tetracyclines, polymyxins. Effective treatments TMP-SMX, quinolones, penicillins, penicillins β-lactamase inhibitors. Rapid diagnosis crucial successful treatment, leading the development of new detection methods such as selective steno medium agar (SMA) molecular techniques nucleic acid detection. Patients S. are prone co-infections other G− bacteria. study by Safdar et al. cancer patients from 1986 2002 showed an increase in infections 2% 7%, making it 5th most commonly isolated G-bacterium this group. infects ICU individuals SARS-CoV-2, pneumonia being frequent clinical manifestation. The COVID-19 pandemic increased intensive care unit admissions mechanical ventilation use, higher infection rates maltophilia.

Language: Английский

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