Dysbiosis of gut microbiota in COVID-19 is associated with intestinal DNA phage dynamics of lysogenic and lytic infection DOI Creative Commons
Aya Ishizaka, Azumi Tamura,

Michiko Koga

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

This study compared intestinal DNA phage dynamics and gut microbiota changes observed at the onset of coronavirus disease 2019 (COVID-19). The participants included 19 healthy individuals patients with severe acute respiratory syndrome 2 infection. Significant differences were in diversity virome after COVID-19 that individuals. Classification by their tail morphology resulted order Caudovirales, a double-stranded phage, accounting for >95% all participants. In classifying phages based on host bacteria, decreased number infecting mainly Clostridia class was immediately recovered over time. After COVID-19, two distinct movement patterns bacteria observed: phage- bacteria-predominant. abundance obligate anaerobes, such as

Language: Английский

Gut microbiota, inflammatory proteins and COVID-19: a Mendelian randomisation study DOI Creative Commons
Y. Chen, Chang Chen

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

Background The human gut microbiota has been identified as a potentially important factor influencing the development of COVID-19. It is believed that disease primarily affects organism through inflammatory pathways. With aim improving early diagnosis and targeted therapy, it crucial to identify specific associated with COVID-19 gain deeper understanding underlying processes. present study sought investigate potential causal relationship between COVID-19, determine extent which proteins act mediators in this relationship. Methods Bidirectional mendelian randomization (MR) Two-step mediated MR analyses were applied examine causative associations among 196 microbiota, 91 main analytical method used was random effects inverse variance weighted (IVW) method. This complemented by Bayesian Mendelian (BWMR) method, utilized test hypothesis MR. In order for results be deemed reliable, statistical significance required both methods. Validation then carried out using an external dataset, further meta-analyses conducted authenticate association reliable. Results our research indicated seven actively risk. Five risk, three positively two negatively Further validation sensitivity analyses. Mediated revealed CCL2 possible mediator causality family Bifidobacteriaceae Bifidobacteriales mediating at ratio 12.73%. Conclusion Suggesting genetic causation emphasizes role CCL2, factor, contributes mechanism action

Language: Английский

Citations

4
Investigating the Role of Gut Microbiota in Pediatric Patients with Severe COVID-19 or MIS-C DOI Creative Commons
Elena Franchitti, Paolo Bottino, Francesca Sidoti

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 83 - 83

Published: Jan. 4, 2025

Severe COVID-19 and MIS-C are rare but serious outcomes associated with SARS-CoV-2 infection. The onset of often involves the gastrointestinal system, suggesting a potential connection gut microbiota. This study aims to compare microbiota children severe those using various biomolecular approaches. Gut composition specific microbial modulations were analyzed fecal samples collected at hospital admission. included hospitalized patients (mean age 6 ± 5 years) diagnosed (37 patients) or patients). Microbial differences assessed both NGS qRT-PCR methodologies. In 75% cases, pharmacological treatments antibiotics corticosteroids, which influenced composition. Early was found have most significant impact on diversity. Significant in alpha beta diversity observed between patients, particularly concerning low-abundance species. Levels Bacteroides spp., Bifidobacterium Akkermansia muciniphila comparable groups, while an increased activity spp. noted positive (p = 0.019). An in-depth evaluation lesser-known species may be key reducing risk developing microbiota-based biomarkers for early diagnosis MIS-C.

Language: Английский

Citations

0
The Gut Virome and Human Health: From Diversity to Personalized Medicine DOI Creative Commons

Rahul Harikumar Lathakumari,

K.V. Leela,

Anusha Gopinathan

et al.

Engineering Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 100191 - 100191

Published: Feb. 1, 2025

Language: Английский

Citations

0
Gut microbe–host interactions in post-COVID syndrome: a debilitating or restorative partnership? DOI Creative Commons
Torsten P. M. Scheithauer,

R.C. Montijn,

Arnout Mieremet

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Sept. 17, 2024

Post-COVID syndrome (PCS) patients have reported a wide range of symptoms, including fatigue, shortness breath, and diarrhea. Particularly, the presence gastrointestinal symptoms has led to hypothesis that gut microbiome is involved in development severity PCS. The objective this review provide an overview role PCS by describing microbial composition metabolites COVID-19 Moreover, host-microbe interactions via microbiota-gut-brain (MGB) microbiota-gut-lung (MGL) axes are described. Furthermore, we explore potential therapeutically targeting support recovery reviewing preclinical model systems clinical studies. Overall, current studies evidence microbiota affected PCS; however, diversity highly individual compositions suggest need for personalized medicine. Gut-targeted therapies, treatments with pre- probiotics, improve quality life individuals.

Language: Английский

Citations

1
Gastrointestinal pathophysiology in long COVID: Exploring roles of microbiota dysbiosis and serotonin dysregulation in post-infectious bowel symptoms DOI Creative Commons
Linda Chia‐Hui Yu

Life Sciences, Journal Year: 2024, Volume and Issue: 358, P. 123153 - 123153

Published: Oct. 23, 2024

Language: Английский

Citations

1
Gut microbial profiling of COVID-19 patients in Uganda DOI
David Patrick Kateete,

Christopher Lubega,

Ronald Galiwango

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 30, 2024

Abstract Background: While COVID-19 spread globally, the role of gut microbiota in patient outcomes has remained an area exploration especially resource limited settings. This study aimed to comprehensively profile microbiome among Ugandan patients and infer potential implications. Methods: Nasopharyngeal swabs, stool, clinical demographic data were collected from confirmed cases at isolation treatment centers Kampala Entebbe, Uganda, during first second waves pandemic Uganda (i.e., 2020 2021, respectively). SARS-CoV-2 presence swab samples was by quantitative real-time RT-PCR assays. 16S rRNA metagenomic next-generation sequencing performed on DNA extracted stool samples, followed bioinformatics analysis. Machine learning used determine microbes that associated with disease severity. Results: We observed varied microbial composition between healthy controls. Potentially pathogenic bacteria such as Klebsiella oxytoca, Salmonella enterica Serratia marcescens had increased states, severe cases. Enrichment opportunistic pathogens, Enterococcus species, depletion beneficial microbes, like Alphaproteobacteria, mild identified age Ruminococcaceae, Bacilli, Enterobacteriales, porphyromonadaceae, Prevotella copri predictive Conclusion: These findings suggest plays a dynamics infection African patients. The shift abundance specific can moderately predict severity this population. Their direct or indirect roles determining should be investigated further for therapeutic interventions.

Language: Английский

Citations

0
Longitudinal Fecal Microbiota Profiles in A Cohort of Non-Hospitalized Adolescents and Young Adults with COVID-19: Associations with SARS-CoV-2 Status and Long-Term Fatigue DOI Creative Commons
Christine Olbjørn, Milada Hagen, Aina Elisabeth Fossum Moen

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(11), P. 953 - 953

Published: Oct. 31, 2024

Adolescents most often experience mild acute COVID-19, but may still face fatigue and persistent symptoms such as post-COVID-19 condition (PCC) post-infective syndrome (PIFS). We explored the fecal microbiota of SARS-CoV-2 positive negative non-hospitalized adolescents young adults (12-25 years age) in "Long-Term Effects COVID-19 Adolescents" (LoTECA) project, a longitudinal observational cohort study. With targeted qPCR approach, quantities 100 bacterial taxa were measured at baseline (early convalescent stage) 145 SARS-CoV-2-positive 32 participants after six months 107 19 participants. Results:

Language: Английский

Citations

0
Dysbiosis of gut microbiota in COVID-19 is associated with intestinal DNA phage dynamics of lysogenic and lytic infection DOI Creative Commons
Aya Ishizaka, Azumi Tamura,

Michiko Koga

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

This study compared intestinal DNA phage dynamics and gut microbiota changes observed at the onset of coronavirus disease 2019 (COVID-19). The participants included 19 healthy individuals patients with severe acute respiratory syndrome 2 infection. Significant differences were in diversity virome after COVID-19 that individuals. Classification by their tail morphology resulted order Caudovirales, a double-stranded phage, accounting for >95% all participants. In classifying phages based on host bacteria, decreased number infecting mainly Clostridia class was immediately recovered over time. After COVID-19, two distinct movement patterns bacteria observed: phage- bacteria-predominant. abundance obligate anaerobes, such as

Language: Английский

Citations

0