Isolation and probiotic evaluation of Apilactobacillus kunkeei and Bombella sp. from Apis mellifera anatoliaca and Bombus terrestris

International Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

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Exploring climate‐related gut microbiome variation in bumble bees: An experimental and observational perspective

Ecology, Journal Year: 2025, Volume and Issue: 106(3)

Published: March 1, 2025

Abstract Rising temperatures negatively affect bumble bee fitness directly through physiological impacts and indirectly by disrupting mutualistic interactions between bees other organisms, which are crucial in determining species‐specific responses to climate change. Gut microbial symbionts, key regulators of host nutrition health, may be the Achilles' heel thermal insects. They not only modulate biotic with plants pathogens but also exhibit varying sensitivity themselves. Understanding how environmental changes disrupt microbiome communities is a first step determine potential consequences for population responses. We analyzed gut bacterial six species inhabiting different climatic niches along an elevational gradient German Alps using 16S ribosomal DNA amplicon sequencing. investigated whether inter‐ intraspecific differences can linked species' niches, differ temperature, flower resource composition, likely pathogen pressure. A reciprocal translocation experiment distinct regions tested Bombus terrestris lucorum change short‐term when exposed new environments. Finally, we these heat cold wave scenarios within chambers disentangle pure temperature‐driven effects on from effects. Interspecific variation composition exceeded variation. Species levels stability, where stability defined as within‐group variance: lower indicated greater variance, predominantly observed higher elevations. Transplanted showed subtle adjustments, marked increase Lactobacillaceae upon exposure warmer regions; however, microbiomes did under laboratory temperature scenarios. conclude that could lead For example, less stable elevations might indicate increased pathogens. Short‐term following relatively microbiomes, such B. , rapidly integrate bacteria, their capacity cope environments

Language: Английский

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Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 8, 2025

The global decline in biodiversity and insect populations highlights the urgent need to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats these essential services. Changes land use alter resource nest site availability, pesticide exposure other factors impacting richness, diversity, health of bee species. In this study, we investigated yet another facet currently less well context: Microbial communities associated with wild bees play crucial roles larval development, metabolism, immunity overall health. However, drivers dynamics healthy microbiome are still poorly understood, especially regarding direct indirect effects on diversity composition microbial communities. We examined bacterial offspring materials Megachilid trap-nesting bee, Osmia bicornis, along a gradient intensification 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, food resources known influence compositions species, hypothesized changes would available for material collection thereby affecting microbiomes their environments. anticipated reduced altered increased which is decrease number resources, including pool floral soil bacteria surrounding environment. As expected, observed shifts nests across varying degrees intensity, differing management types availability flowers. Shannon (larval pollen provision, enclosure) guts decreased increasing intensity. pupae remained unaffected, indicating reorganization during metamorphosis, not significantly influenced resources. Our findings provide new insights into shaping environmental transmission microbiomes. This understanding comprehending impacts intensive developing strategies mitigate effects.

Language: Английский

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Captive-rearing changes the gut microbiota of the bumblebee Bombus lantschouensis native to China

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18964 - e18964

Published: Feb. 13, 2025

Bumblebees play crucial roles as pollinators in both natural agricultural and ecological systems. Their health overall fitness are significantly affected by the gut microbiota, which can maintain intestinal homeostasis hosts regulating their nutritional metabolism. However, information about diversity of microbiota related functional changes during artificial rearing native species is currently limited. This study investigated dynamic remodeling Chinese bumblebee Bombus lantschouensis under captive rearing, supported 16S rRNA amplicon sequencing bacterial DNA. The typical microbial community composition was detected wild B. , with genus Gilliamella Snodgrassella identified dominant strains. Conversely, captive-reared group showed increased decreased abundance certain microorganisms. populations Bifidobacterium Saccharibacter Lactobacillus including Firm-4 Firm-5, were dramatically after captive-rearing became bacteria, while strikingly reduced. Notably, this found that pathogenic bacteria appeared intestines wild-caught disappeared when host reared conditions. shows bumblebees facilitates physiological metabolism commercial insects.

Language: Английский

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Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

The global decline in biodiversity and insect populations highlights the urgent to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats these essential services. Changes land use alter resource nest site availability, pesticide exposure other factors impacting richness, diversity, health of bee species. In this study, we investigated yet another facet currently less well context: Microbial communities associated with wild bees play crucial roles larval development, metabolism, ­immunity overall health. However, drivers dynamics healthy microbiome are still poorly understood, especially regarding direct indirect effects on diversity composition microbial communities. We examined bacterial offspring materials Megachilid trap-nesting bee, O. bicornis,along a gradient intensification 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, food resources known influence compositions species, hypothesized changes would available for material collection thereby affecting microbiomes their environments. anticipated reduced altered increased which is decrease number resources, including pool floral soil bacteria surrounding environment. As expected, observed shifts nests across varying degrees intensity, differing management types availability flowers. Shannon (larval pollen provision, enclosure) guts decreased increasing intensity. pupae remained unaffected, indicating reorganization during metamorphosis, not significantly influenced resources. Our findings provide new insights into shaping environmental transmission microbiomes. This understanding comprehending impacts intensive developing strategies mitigate effects.

Language: Английский

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The Gut Microbiome of Two Wild Bumble Bee Species Native of South America: Bombus pauloensis and Bombus bellicosus

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Sept. 28, 2024

Language: Английский

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