In-depth characterization of Klebsiella pneumoniae carbapenemase (KPC)-encoding plasmids points at transposon-related transmission of resistance genes

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 13, 2025

Antimicrobial resistance (AMR) is a growing threat in healthcare systems, particularly the management of infections critically ill patients. This study highlights how to identify clusters and putative sharing mobile genetic elements, such as transposons, hospital setting using long-read whole genome sequencing (lrWGS). The approach described here can be employed investigate transmission dynamics KPC-3-positive Klebsiella pneumoniae at multiple levels, from entire isolate down individual plasmids transposons. Here, bla KPC-3 harboring transposon cluster was identified by Mash-based distance calculation for plasmids. used local accumulation on surgical infectious disease wards tertiary care center Germany over time six months. In total, seven patients were affected. Core multi-locus sequence typing analysis (cgMLST) two distinct clusters: type (ST) 307 (n = 5) ST101 2). All isolates carried carbapenemase. Further Mash distance-based plasmid not consistent with transfer due heterogeneity, but across all isolates. Infection control evaluation patient movements within their admission supports possible clonal transmission. Subsequent infection measures, including point prevalence screening enhanced contact precautions, successfully contained further transmissions. illustrates value in-depth understanding epidemiology AMR, environments.

Language: Английский

The dark matter of bacterial genomic surveillance—antimicrobial resistance plasmid transmissions in the hospital setting

Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

ABSTRACT Dissemination of antimicrobial resistance (AMR) is a growing global public health burden. The aim this study was to characterize AMR plasmid transmissions within tertiary care hospital and identify relevant transmission pathways. During an 18-month observation period, 540 clinical gram-negative multidrug-resistant bacterial (MDRB) isolates were collected during routine surveillance subjected Pacific Biosciences long-read whole genome sequencing. Potential clonal determined based on core multilocus sequence typing (cgMLST), detected using novel real-time applicable tool for detection. validated epidemiological data. Among the 471 eligible MDRB isolates, we 1,539 plasmids; 84.41% these circularized. We identified 38 potential in 24 clusters cgMLST 121 containing genetically related plasmids. latter clusters, 10 contained different types (involving 2–38 median: 3 isolates), nine multiple species (2–18 4). Epidemiological data confirmed 19 (in seven clusters) additional 12 (within eight clusters). these, cases intra-host five patient-to-patient transmissions. demonstrate that plasmids can be by combining sequencing with tools molecular surveillance. In addition, our highlights more than decade genomic missed at least one-third all events due IMPORTANCE Antimicrobial poses significant threat human health. Most determinants are encoded extra-chromosomally Although current infection control strategies primarily focus bacteria, until today, routes neither understood nor analyzed setting. study, simultaneously both clonal, is, chromosomes, software Our analysis not only yet unknown healthcare facilities or community but also resulted, addition transmissions, third

Language: Английский

Large-scale genomic analysis of Elizabethkingia anophelis

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 29, 2024

Language: Английский