High fat diet induces differential age- and gender-dependent changes in neuronal function linked to redox stress DOI

Megan de Lange,

Vladyslava Yarosh,

Kevin Farell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

Abstract The prevalence of neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, is steadily increasing, posing significant challenges to global healthcare systems. Emerging evidence suggests that dietary habits, particularly consumption high-fat diets specify which fats, may play a pivotal role in the development progression disorders. Moreover, several studies have shed light on intricate communication between gut brain, known gut-brain axis its involvement processes. This study aims assess effects intake various aspects neuronal function during aging following gender separation help understand potential contributions diet function. To investigate diet, Drosophila melanogaster was used exposed standard normal food (NF) (HF). Adults were grouped at 10 45 days age male female flies reared under same conditions. Multiple assays conducted, showing differential gender- HF diet-induced oxidative stress levels determined by malondialdehyde (MDA) measurements, enhanced caspase-3 expression reduced climbing activity. Adult lifespan both conditions unchanged but odour-associated learning ability larvae diet. first characterise phenotypes an age- gender-specific manner model. Our findings suggest induces forms dysfunction with sex-specific outcomes, characterised cell death impacts behavioural functions.

Language: Английский

Multi-omics analysis explore the mechanism of deoxynivalenol inhibiting rabbit appetite through microbial-gut-brain axis DOI Creative Commons

Xuejun Yuan,

Wenjie Chen, Rui Li

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 291, P. 117849 - 117849

Published: Feb. 1, 2025

Deoxynivalenol (DON) is one of the most prevalent mycotoxins and can induce weight loss vomiting in animals. This study aimed to elucidate molecular mechanisms underlying DON-induced anorexia rabbits through microbial-gut-brain axis. A total forty were randomly divided into a DON group control group, receiving 1.5 mg/kg.BW an equivalent volume saline daily via intragastric administration, respectively. The pre-treatment period lasted for 7 days, followed by formal experimental 24 days. findings revealed that exposure significantly reduced food intake gain, leading intestinal apoptosis barrier injuries. 16S rRNA sequencing data indicated decreased diversity richness cecal microflora, suppressed probiotic populations, disrupted host lipid metabolism. RNA-Seq iTRAQ used cross-analyze shared differentially expressed genes (DEGs) proteins (DEPs) hypothalamus jejunum, along with their enrichment pathways, indicating TNF-α, NF-κB, NPY may play pivotal roles anorexia. qRT-PCR Western blotting results confirmed significant increases mRNA protein expression levels NF-κB 5-HT2A both jejunum. In conclusion, ingestion disrupt barrier, up-regulate IFN-γ, intestine, trigger immuno-inflammatory response axis, ultimately resulting appetite suppression loss.

Language: Английский

Citations

1
High fat diet induces differential age- and gender-dependent changes in neuronal function in Drosophila linked to redox stress DOI Creative Commons

Megan de Lange,

Vladyslava Yarosh,

Kevin Farell

et al.

Behavioural Brain Research, Journal Year: 2025, Volume and Issue: unknown, P. 115510 - 115510

Published: Feb. 1, 2025

Language: Английский

Citations

0
Drosophila symbionts in infection: when a friend becomes an enemy DOI Creative Commons
Yi Yu, Igor Iatsenko

Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The insect microbiome is comprised of extracellular microbial communities that colonize the host surfaces and endosymbionts reside inside cells tissues. Both these participate in essential aspects biology, including immune response interactions with pathogens. In recent years, our knowledge about role infection has increased tremendously. While many studies have highlighted microbiome's protective effect against various natural enemies insects, unexpected discoveries shown some members microbiota can facilitate pathogenic infections. Here, we summarize fruit fly, Drosophila melanogaster, substantially progressed understanding host-pathogen-microbiome during infection. We on mechanisms gut microbiota, highlight examples exploitation by pathogens, detail endosymbiont-mediated protection. addition, delve into a previously neglected topic research-the crosstalk between microbiota. Finally, address how remain resilient to responses stably By examining influenced reciprocally affects outcomes, this review provides timely cohesive coverage roles

Language: Английский

Citations

0
High fat diet induces differential age- and gender-dependent changes in neuronal function linked to redox stress DOI

Megan de Lange,

Vladyslava Yarosh,

Kevin Farell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

Abstract The prevalence of neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, is steadily increasing, posing significant challenges to global healthcare systems. Emerging evidence suggests that dietary habits, particularly consumption high-fat diets specify which fats, may play a pivotal role in the development progression disorders. Moreover, several studies have shed light on intricate communication between gut brain, known gut-brain axis its involvement processes. This study aims assess effects intake various aspects neuronal function during aging following gender separation help understand potential contributions diet function. To investigate diet, Drosophila melanogaster was used exposed standard normal food (NF) (HF). Adults were grouped at 10 45 days age male female flies reared under same conditions. Multiple assays conducted, showing differential gender- HF diet-induced oxidative stress levels determined by malondialdehyde (MDA) measurements, enhanced caspase-3 expression reduced climbing activity. Adult lifespan both conditions unchanged but odour-associated learning ability larvae diet. first characterise phenotypes an age- gender-specific manner model. Our findings suggest induces forms dysfunction with sex-specific outcomes, characterised cell death impacts behavioural functions.

Language: Английский

Citations

0