Constructing a label-free electrochemical biosensor based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanosheets for the sensitive detection of VKORC1*2 gene DOI Creative Commons
Jie Wang, Hezhong Ouyang, Zhihao Xu

et al.

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(8), P. 105848 - 105848

Published: May 29, 2024

Pharmacogenetic testing technology can effectively determine the individual differences among patients and scientifically assist doctors to select most suitable drugs for each patient. In this study, an electrochemical biosensor based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanosheets was constructed detect Vitamin K epoxide reductase complex 1 type AA (VKORC1*2). Firstly, we fabricated as substrate material biosensors. Subsequently, surface modified with Au nanoparticles facilitate connection of sensing probes enhance current signal amplification. The showed a negative linear relationship concentrations target DNA (tDNA, VKORC1*2 gene) within pM − μM, limit detection (LOD) = 0.36 pM, quantification (LOQ) 1.19 pM. demonstrated exceptional specificity, reproducibility, stability. real sample analysis, it exhibited recovery range 96.63 % 110.57 (RSD ≤ 3.07 %) various tDNA concentrations, thereby indicating its promising potential in clinical diagnostics.

Language: Английский

RPA-CRISPR-Cas-Mediated Dual Lateral Flow Assay for the Point-of-Care Testing of HPV16 and HPV18 DOI

K. Zhang,

Qingmei Li, Kun Wang

et al.

Bioconjugate Chemistry, Journal Year: 2024, Volume and Issue: 35(11), P. 1797 - 1804

Published: Oct. 15, 2024

The incidence of cervical cancer caused by human papillomavirus (HPV) infection has increased in recent years. More than half all cases are due to HPV16 and HPV18 infection, so testing is essential prevent cancer. HPV mainly carried out hospitals, but it subject time specialized medical facilities. On the other hand, home self-testing using simple diagnostics would present an attractive alternative privacy flexibility with regard place, provided sufficient sensitivity specificity can be achieved. In this work, a dual lateral flow assay based on RPA-CRISPR-Cas12a/13a (named RC-LFA) for detection was described. Taking advantage cleavage Cas12a Cas13a, CRISPR-Cas12a/Cas13a system designed detect HPV18. strip two test lines suit CRISPR-Cas12a/Cas13 system. RC-LFA achieves rapid simultaneous high (10 copies/μL) about 40 min from extraction nucleic acid instrument-free readout. user-friendly instrument-free, making promising method self-tests at home.

Language: Английский

Citations

4
Human Papillomavirus (HPV) Vaccination: Progress, Challenges, and Future Directions in Global Immunization Strategies DOI Creative Commons
Francesco Branda, Grazia Pavia, Alessandra Ciccozzi

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1293 - 1293

Published: Nov. 19, 2024

Human papillomavirus (HPV) is a widespread viral pathogen, responsible for significant burden of cervical and other cancers worldwide. Over the past decades, development adoption prophylactic HPV vaccines have dramatically reduced incidence HPV-related diseases. However, despite efficacy these vaccines, global immunization efforts still face several obstacles, including low vaccination coverage in low- middle-income countries, vaccine hesitancy, disparities access to healthcare. This review aims provide comprehensive overview current state their mechanisms action, safety profiles, real-world efficacy. We will explore impact on cancer prevention, examine challenges related distribution uptake, assess role public health policies improving rates. Additionally, highlight latest advancements therapeutic ongoing research into next-generation potential strategies context personalized medicine. By examining factors, we aim insights future directions its health.

Language: Английский

Citations

4
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors DOI Creative Commons

Xiaonan Xin,

Jing Su, Haoran Cui

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 155 - 155

Published: March 2, 2025

High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, environmental monitoring. CRISPR/Cas systems have emerged as transformative tools biosensing due to their unparalleled specificity, programmability, unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target guided by specific protospacers, which ensures accurate target recognition, bystander activity triggered upon binding, enables robust signal amplification. These properties make highly versatile designing biosensors ultra-sensitive detection. This review comprehensively explores recent advancements system-based biosensors, highlighting impact on improving performance. We discuss the integration of with diverse readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), so on. Additionally, we examine development integrated systems, such microfluidic devices portable leverage technology point-of-care testing (POCT) high-throughput analysis. Furthermore, identify unresolved challenges, aiming inspire innovative solutions accelerate translation these technologies into practical diagnostics, food, environment safety.

Language: Английский

Citations

0
Advancements in fluorescent nanobiosensors for HPV detection: from integrating nanomaterials to DNA nanotechnology DOI
Farhad Bani, Farhad Bani, Balal Khalilzadeh

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143619 - 143619

Published: April 1, 2025

Language: Английский

Citations

0
Constructing a label-free electrochemical biosensor based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanosheets for the sensitive detection of VKORC1*2 gene DOI Creative Commons
Jie Wang, Hezhong Ouyang, Zhihao Xu

et al.

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(8), P. 105848 - 105848

Published: May 29, 2024

Pharmacogenetic testing technology can effectively determine the individual differences among patients and scientifically assist doctors to select most suitable drugs for each patient. In this study, an electrochemical biosensor based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanosheets was constructed detect Vitamin K epoxide reductase complex 1 type AA (VKORC1*2). Firstly, we fabricated as substrate material biosensors. Subsequently, surface modified with Au nanoparticles facilitate connection of sensing probes enhance current signal amplification. The showed a negative linear relationship concentrations target DNA (tDNA, VKORC1*2 gene) within pM − μM, limit detection (LOD) = 0.36 pM, quantification (LOQ) 1.19 pM. demonstrated exceptional specificity, reproducibility, stability. real sample analysis, it exhibited recovery range 96.63 % 110.57 (RSD ≤ 3.07 %) various tDNA concentrations, thereby indicating its promising potential in clinical diagnostics.

Language: Английский

Citations

0