Using Nano-Luciferase Binary (NanoBiT) Technology to Assess the Interaction Between Viral Spike Protein and Angiotensin-Converting Enzyme II by Aptamers DOI Creative Commons
Meng‐Wei Lin, Cheng‐Han Lin,

Hua-Hsin Chiang

et al.

BioTech, Journal Year: 2025, Volume and Issue: 14(1), P. 20 - 20

Published: March 15, 2025

Nano-luciferase binary technology (NanoBiT)-based pseudoviral sensors are innovative tools for monitoring viral infection dynamics. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells via its trimeric surface spike protein, which binds to the human angiotensin-converting enzyme II (hACE2) receptor. This interaction is crucial entry and serves as a key target therapeutic interventions against disease 2019 (COVID-19). Aptamers, short single-stranded DNA (ssDNA) or RNA molecules, highly specific, high-affinity biorecognition elements detecting infective pathogens. Despite their potential, optimizing assays using traditional protein-protein (PPI) methods often face challenges in assays. In this study, we selected evaluated aptamers ability interact with proteins, enabling dynamic visualization of progression. The NanoBiT-based sensor demonstrated rapid increase luminescence within 3 h, offering real-time measure infection. A comparison detection technologies, including green fluorescent protein (GFP), luciferase, NanoBiT technologies PPI between hACE2, highlighted NanoBiT's superior sensitivity performance, particularly aptamer selection. bioluminescent system provides robust, sensitive, early-stage quantitative approach studying

Language: Английский

An Electrochemical Quinine Detection Approach Based on Small Molecule Promoted Split Aptamer Click Ligation Reaction DOI
Xuan Luo,

Susu Cui,

Weiwei Yang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 127916 - 127916

Published: March 9, 2025

Language: Английский

Citations

0
AptERA 2 targets ERA from Staphylococcus aureus and limits GTP hydrolysis DOI Creative Commons
Katherin Peñaranda,

N.Z. Pereira,

Orestis Savva

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Ribosome assembly is a multistep process that ensures functional ribosome structure. The molecular mechanism ribosome­associated GTPases (RA­GTPases) use to enhance accuracy, remains largely be elucidated. Here, we systematic evolution of ligands by exponential enrichment (SELEX), followed sequencing, comprehensive bioinformatics analysis, and biochemical characterization identify aptamers target the RA-GTPase ERA Staphylococcus aureus. ELONA thermophoresis assays show AptERA 2 interaction with in 200 nM range affinity, displays high level specificity, depends on Docking suggests interacts protein's KH domain, consistent aptamer's similarities helix 45 16S rRNA. did not interact similar RbgA, conserved at GTPase core but lacking confirming aptamer recognizes binds domain ERA. This leads significant reduction 30S-dependent GTP hydrolysis, indicative allosteric modulation enzyme activity or limiting 3’ end rRNA rather than directly blocking binding. Altogether, this work highlights versatility as tools understand complex processes biogenesis further, offering new insights into bacterial protein synthesis mechanisms.

Language: Английский

Citations

0
Using Nano-Luciferase Binary (NanoBiT) Technology to Assess the Interaction Between Viral Spike Protein and Angiotensin-Converting Enzyme II by Aptamers DOI Creative Commons
Meng‐Wei Lin, Cheng‐Han Lin,

Hua-Hsin Chiang

et al.

BioTech, Journal Year: 2025, Volume and Issue: 14(1), P. 20 - 20

Published: March 15, 2025

Nano-luciferase binary technology (NanoBiT)-based pseudoviral sensors are innovative tools for monitoring viral infection dynamics. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells via its trimeric surface spike protein, which binds to the human angiotensin-converting enzyme II (hACE2) receptor. This interaction is crucial entry and serves as a key target therapeutic interventions against disease 2019 (COVID-19). Aptamers, short single-stranded DNA (ssDNA) or RNA molecules, highly specific, high-affinity biorecognition elements detecting infective pathogens. Despite their potential, optimizing assays using traditional protein-protein (PPI) methods often face challenges in assays. In this study, we selected evaluated aptamers ability interact with proteins, enabling dynamic visualization of progression. The NanoBiT-based sensor demonstrated rapid increase luminescence within 3 h, offering real-time measure infection. A comparison detection technologies, including green fluorescent protein (GFP), luciferase, NanoBiT technologies PPI between hACE2, highlighted NanoBiT's superior sensitivity performance, particularly aptamer selection. bioluminescent system provides robust, sensitive, early-stage quantitative approach studying

Language: Английский

Citations

0