Exploring the longitudinal expression dynamics of midguts in adult female Amblyomma americanum ticks DOI Creative Commons
Stephen Lu, Lucas Christian de Sousa‐Paula, José M. C. Ribeiro

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 24, 2024

Abstract Background Female ticks remain attached to their host for multiple days complete a blood meal. This prolonged feeding period is accompanied by significant increase in the tick’s size and body weight, paralleled noteworthy changes tick midgut. While midgut recognized its established role storage processing, importance extends playing crucial acquisition, survival, proliferation of pathogens. Despite this, our overall understanding biology limited. Results Our transcriptome analysis identified 15,599 putative DNA coding sequences (CDS), which were classified into 26 functional groups. Dimensional differential expression analyses revealed four primary transcriptional profiles corresponding unfed, slow-feeding, transitory (from slow- rapid-feeding), rapid-feeding stages. Additionally, comparing current dataset with previously deposited from other species allowed identification commonly expressed transcripts across different Conclusion findings provide detailed temporal resolution numerous metabolic pathways A. americanum adult females throughout process, highlighting dynamic regulation as progresses. Furthermore, we conserved three that exhibit similar patterns. knowledge not only enhances physiological processes within but also opens up potential avenues developing control methods target species.

Language: Английский

Identification and characterization of a Relish-type NF-κB, DvRelish, in Dermacentor variabilis in response to Rickettsia rickettsii infection DOI Creative Commons
Chanida Fongsaran, Victoria I. Verhoeve,

Krit Jirakanwisal

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 15, 2024

Ixodid ticks serve as hosts and transmission vectors for several obligate intracellular bacteria, including members of the spotted fever group (SFG) Rickettsia . Although generate an immune response to bacterial insults, many signaling molecules associated with how they may contribute vector competence are undefined. In this study, we isolated a full-length dvrelish transcript from Dermacentor variabilis , which encoded Relish-type NF-κB. The presence canonical Rel homology domain (RHD) consistent NF-κB proteins suggested role in tick DvRelish. expression DvRelish was confirmed tissues fluorescent microscopy hemocytes indicated increased following infection compared non-tick-borne pathogen. To further determine effect dvRelish gene knockdown on rickettsial infection, used RNA interference-mediated D. demonstrated that transcription decreased after 24 h post-injection siRNA. We then assessed when exposed rickettsii determined inversely loads at 48 post-exposure. Further studies required broaden understanding differential responses SFG elucidate played by arthropod system competence.

Language: Английский

Citations

1
Exploring the longitudinal expression dynamics of midguts in adult female Amblyomma americanum ticks DOI Creative Commons
Stephen Lu, Lucas Christian de Sousa‐Paula, José M. C. Ribeiro

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 24, 2024

Abstract Background Female ticks remain attached to their host for multiple days complete a blood meal. This prolonged feeding period is accompanied by significant increase in the tick’s size and body weight, paralleled noteworthy changes tick midgut. While midgut recognized its established role storage processing, importance extends playing crucial acquisition, survival, proliferation of pathogens. Despite this, our overall understanding biology limited. Results Our transcriptome analysis identified 15,599 putative DNA coding sequences (CDS), which were classified into 26 functional groups. Dimensional differential expression analyses revealed four primary transcriptional profiles corresponding unfed, slow-feeding, transitory (from slow- rapid-feeding), rapid-feeding stages. Additionally, comparing current dataset with previously deposited from other species allowed identification commonly expressed transcripts across different Conclusion findings provide detailed temporal resolution numerous metabolic pathways A. americanum adult females throughout process, highlighting dynamic regulation as progresses. Furthermore, we conserved three that exhibit similar patterns. knowledge not only enhances physiological processes within but also opens up potential avenues developing control methods target species.

Language: Английский

Citations

1