Application of the metagenomic next-generation sequencing technology to identify the causes of pleural effusion

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: March 19, 2025

Background Pleural effusion (PE), frequently encountered in clinical practice, can arise from a variety of underlying conditions. Accurate differential diagnosis PE is crucial, as treatment and prognosis are heavily dependent on the etiology. However, diagnosing cause remains challenging, relying mycobacteriological methods that lack sensitivity time-consuming, or histological examinations require invasive biopsies. The recent advancements metagenomic next-generation sequencing (mNGS) have shown promising applications infectious diseases. Despite this, there limited research utility mNGS comprehensive diagnostic tool for simultaneously identifying causes PE, particularly cases tuberculosis malignancy. Methods This study aimed to assess efficacy detecting tuberculous pleural (TPE) malignant (MPE). A total 35 patients with were included, their samples analyzed using mNGS. Results Among participants, 8 ultimately diagnosed TPE, 10 MPE, lung adenocarcinoma being most prevalent pathological type (50%, 5/10), according established criteria. Additionally, 7 non-infectious PE. identified only 2 TPE MPE. Mycobacterium was 25% (2/8), while specificity 100%. For tumor detection, demonstrated 80%, 92.6%, an AUC 0.882. Conclusion effective distinguishing MPE non-MPE, but not suitable TPE.

Language: Английский

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Current methods in the diagnosis of invasive meningococcal disease

Frontiers in Pediatrics, Journal Year: 2025, Volume and Issue: 13

Published: April 22, 2025

Invasive meningococcal disease (IMD) remains a significant health concern due to its global distribution, potential for epidemic spread, unpredictable nature, rapid progression, and high mortality rates or permanent sequelae. The elimination of illness via immunization is primary objective the World Health Organization's strategy defeat meningitis by 2030. Timely recognition infection immediate, precise, specific identification Neisseria meningitidis are essential optimal clinical management enhanced outcomes, monitoring evolving epidemiology, detecting outbreak activity, providing an effective public response. Clinical findings, microscopic Gram stains, cultures traditional widely used diagnostic methods definition IMD, despite some disadvantages. Real-time polymerase chain reaction (rt-PCR) whole genome sequencing (WGS) more accurate techniques N. subsequent investigation; however, their cost limited availability present issues. WGS has numerous uses, including strain characterization, population genomics, antibiotic resistance monitoring, investigation. New-generation molecular technologies have been will be designing vaccines, as well monitor dynamic seroepidemiology. Microbiology reference laboratories important, digital records expertise they provide benefit meningitidis, other pathogens. While there progress in development tools, it probable that combination approaches new strategies still necessary. goal this review was evaluate current diagnosing IMD discuss challenges practice.

Language: Английский

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A Rare Case of Severe Pneumonia Caused by Leptospirosis Complicated with Jarisch-Herxheimer Reaction

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 113(1), P. 116891 - 116891

Published: May 3, 2025

Language: Английский

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Case report: diagnosis of neurobrucellosis in a non-endemic area child using metagenomic next-generation sequencing

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Language: Английский

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