On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform DOI Creative Commons
Huijun Lu, Tong Zhang, Wei‐Fone Huang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 976 - 976

Published: April 24, 2025

Acinetobacter baumannii (A. baumannii), a very common pathogen, poses significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. carbapenem-resistant (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence air is of great significance, especially hospitals. Herein, we developed Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release nucleic acid test. Combined with wet cyclone sampler, detect airborne most gene, blaOXA-23, within 70 min. This CLC has also been proven have detection limit 6 × 102 CFU CRAB per test simulated samples. Moreover, this was used five actual samples from tertiary hospital, results achieved perfect concordance sequencing data, validating platform’s accuracy reliability. showed potential for rapid, on-site gene offering valuable tool control

Language: Английский

On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform DOI Creative Commons
Huijun Lu, Tong Zhang, Wei‐Fone Huang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 976 - 976

Published: April 24, 2025

Acinetobacter baumannii (A. baumannii), a very common pathogen, poses significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. carbapenem-resistant (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence air is of great significance, especially hospitals. Herein, we developed Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release nucleic acid test. Combined with wet cyclone sampler, detect airborne most gene, blaOXA-23, within 70 min. This CLC has also been proven have detection limit 6 × 102 CFU CRAB per test simulated samples. Moreover, this was used five actual samples from tertiary hospital, results achieved perfect concordance sequencing data, validating platform’s accuracy reliability. showed potential for rapid, on-site gene offering valuable tool control

Language: Английский

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