Chemical Engineering & Technology,
Journal Year:
2022,
Volume and Issue:
46(4), P. 766 - 775
Published: Dec. 21, 2022
Abstract
Cofactors,
which
are
key
components
in
many
bio‐based
processes,
often
consumed
within
the
biosynthetic
reaction,
but
usually
converted
back
to
their
original
form
by
recycling.
This
study
presents
an
approach
for
selective
removal
of
cofactors
from
aqueous
solution
at
up
submillimolar
concentrations.
The
rare
earth
elements
(REE)
scandium,
yttrium,
and
lanthanum,
oxidation
state
+3,
used
sparingly
soluble
salts
with
investigated
cofactors,
pyridoxal‐5‐phosphate,
NAD
+
,
NADP
adenosine
triphosphate,
thiamine
pyrophosphate.
Up
>
99
%
efficiency
via
cofactor
salt
precipitation
was
observed.
Redissolution
recovery
formed
is
obtained
treatment
NaH
2
PO
4
a
metathesis
forming
solubilized
corresponding
REE
phosphate
salts.
ChemSusChem,
Journal Year:
2023,
Volume and Issue:
16(23)
Published: June 26, 2023
Unspecific
peroxygenases
have
attracted
interest
in
synthetic
chemistry,
especially
for
the
oxidative
activation
of
C-H
bonds,
as
they
only
require
hydrogen
peroxide
(H2
O2
)
instead
a
cofactor.
Due
to
their
instability
even
small
amounts
H2
,
different
strategies
like
enzyme
immobilization
or
situ
production
been
developed
improve
stability
these
enzymes.
While
most
studied
separately,
combination
photocatalysis
with
immobilized
enzymes
was
recently
reported.
To
show
advantages
and
limiting
factors
photobiocatalytic
reaction,
comparison
is
made
between
free
Adjustment
critical
parameters
such
(i)
substrate
concentration,
(ii)
illumination
wavelength
(iii)
light
intensity
results
significantly
increased
stabilities
variant.
Moreover,
under
optimized
conditions
turnover
number
334,500
reached.
Physical Sciences Reviews,
Journal Year:
2023,
Volume and Issue:
9(8), P. 2737 - 2755
Published: May 31, 2023
Abstract
The
application
potential
of
enzymes
is
undoubtedly
very
high.
However,
despite
the
large
number
different
and
enzyme
activities,
industrial
processes
comparatively
small.
particular
challenge
often
lies
in
transferring
promising
laboratory
to
an
scale.
Here,
required
performance
parameters,
such
as
stability
or
productivity,
must
be
achieved.
On
one
hand,
this
can
achieved
by
improving
enzymes.
other
key
indicators
only
using
technical
systems
sense
process
intensification.
In
enzymatic
processes,
immobilization
means
choice
enable
processes.
aim
article
outline
most
important
methods
summarize
immobilized
Finally,
are
compared
a
case
study
with
unspecific
peroxygenase.
Physical Sciences Reviews,
Journal Year:
2024,
Volume and Issue:
9(10), P. 3273 - 3287
Published: Feb. 28, 2024
Abstract
Strategies
to
reduce
cost
and
emission
profiles
are
becoming
increasingly
important
for
the
development
of
affordable
sustainable
bio-based
production.
The
overall
objective
process
intensification
in
different
industries
is
achieve
substantial
benefits
terms
cost,
product
concentration
quality,
while
eliminating
waste
improving
safety.
Intensification
bioprocesses
could
be
a
valuable
tool
enhancing
efficiency
reducing
resource
consumption
bioproduction.
In
general,
bioprocess
defined
as
an
increase
bioproduct
output
relative
cell
concentration,
time,
reactor
volume
or
cost.
This
brief
overview
provides
definition
biotechnology,
presents
several
general
specific
examples,
addresses
some
current
challenges.
Chemical Engineering & Technology,
Journal Year:
2022,
Volume and Issue:
46(4), P. 766 - 775
Published: Dec. 21, 2022
Abstract
Cofactors,
which
are
key
components
in
many
bio‐based
processes,
often
consumed
within
the
biosynthetic
reaction,
but
usually
converted
back
to
their
original
form
by
recycling.
This
study
presents
an
approach
for
selective
removal
of
cofactors
from
aqueous
solution
at
up
submillimolar
concentrations.
The
rare
earth
elements
(REE)
scandium,
yttrium,
and
lanthanum,
oxidation
state
+3,
used
sparingly
soluble
salts
with
investigated
cofactors,
pyridoxal‐5‐phosphate,
NAD
+
,
NADP
adenosine
triphosphate,
thiamine
pyrophosphate.
Up
>
99
%
efficiency
via
cofactor
salt
precipitation
was
observed.
Redissolution
recovery
formed
is
obtained
treatment
NaH
2
PO
4
a
metathesis
forming
solubilized
corresponding
REE
phosphate
salts.
