Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids DOI Creative Commons
Javier Santiago‐Arcos, Susana Velasco‐Lozano,

Eleftheria Diamanti

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 23, 2023

Abstract Cell-free biocatalysis is gaining momentum in the production of value-added chemicals, particularly stepwise reaction cascades. However, stability enzyme cascades industrial settings often compromised when using free enzymes. In this study, we have developed a stable multifunctional heterogeneous biocatalyst co-immobilizing five enzymes on microparticles to transform 1,ω-diols into 1,ω-hydroxy acids. We improved operational efficiency and by fine-tuning loading spatial organization. Stability issues are overcome through post-immoblization polymer coating. The general applicability demonstrated its scale-up both batch packed bed reactors, allowing product yield > 80%. continuous process fed with H2O2 as oxygen source, reaching Space-Time Yield (STY) 0.76 g·L− 1·h− 1, maintained for first 12 hours. Finally, flow system telescoped second plug-flow reactor different biocatalyst. As result, 6-enzyme 2-reactor sequentially transforms 1,ω-aminoacids while in-situ recycling NAD+, depleting generating O2.

Language: Английский

Intensification of a biocatalytic oxidation under fine bubble aeration in a rotating bed reactor DOI Creative Commons
Zeynep Perçin,

Lotta Kursula,

E. A. Lofgren

et al.

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 207, P. 109333 - 109333

Published: April 15, 2024

The utilization of oxidases suffers from insufficient oxygen availability due to its low solubility in gas-liquid multiphase systems. To overcome this limitation, is continuously supplied the reaction medium during course reaction, however, it causes significant gas consumption. In study, a novel experimental set-up was established by installing sintered frit porous sparger SpinChem® rotating bed reactor. Covalently immobilized glucose oxidase on epoxy-functionalized carriers used reactor with an activity yield immobilization over 98%. For fine bubble aeration, volumetric mass transfer coefficient kLa (67.3 h-1) tripled compared macrobubble aeration (22.5 h-1), while volume-specific rate remained constant. A maximum 96.5% reached. improved factor 12.9 for under identical conditions. With consumption reduced 87.5% establishing same enzyme-specific rates at comparable kLa. Validation carried out repetitive batches, achieving specific activity. This study demonstrates advantages and presents promising results addressing sustainable biotransformation processes.

Language: Английский

Citations

1
Oxidation of benzyl alcohol derivatives into carboxylic acids with a new Acetobacter malorum strain: boosting the productivity in a continuous flow system DOI
Luca Nespoli, Silvia Donzella,

Emma Ribul Moro

et al.

Molecular Catalysis, Journal Year: 2024, Volume and Issue: 571, P. 114698 - 114698

Published: Nov. 26, 2024

Language: Английский

Citations

0
Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids DOI Creative Commons
Javier Santiago‐Arcos, Susana Velasco‐Lozano,

Eleftheria Diamanti

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 23, 2023

Abstract Cell-free biocatalysis is gaining momentum in the production of value-added chemicals, particularly stepwise reaction cascades. However, stability enzyme cascades industrial settings often compromised when using free enzymes. In this study, we have developed a stable multifunctional heterogeneous biocatalyst co-immobilizing five enzymes on microparticles to transform 1,ω-diols into 1,ω-hydroxy acids. We improved operational efficiency and by fine-tuning loading spatial organization. Stability issues are overcome through post-immoblization polymer coating. The general applicability demonstrated its scale-up both batch packed bed reactors, allowing product yield > 80%. continuous process fed with H2O2 as oxygen source, reaching Space-Time Yield (STY) 0.76 g·L− 1·h− 1, maintained for first 12 hours. Finally, flow system telescoped second plug-flow reactor different biocatalyst. As result, 6-enzyme 2-reactor sequentially transforms 1,ω-aminoacids while in-situ recycling NAD+, depleting generating O2.

Language: Английский

Citations

0