ADSC-Exs Suppresses the Fibrosis Process of Derma in Secondary Lymphedema DOI Creative Commons

Xinxin Wang,

Yilan Li, Jianping Ye

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Background Mesenchymal stem cells (MSCs) and their exosomes, particularly adipose-derived cell exosomes (ADSC-Exs), have shown promise in treating secondary lymphedema (SLE), a condition characterized by fibrosis driven the TGFβ-Smad signaling pathway. While ADSCs ADSC-Exs demonstrated antifibrotic effects, it is not yet clear whether these benefits from ability to regulate this This study aimed clarify role of reducing SLE modulating Methods We established model C57BL/6 mice through surgical excision localized radiation. Tissue staining was used assess progression at key time points, identifying peak stage. were injected into affected areas test therapeutic while TGFβ1 inhibitors as controls block compared effects ADSCs, ADSC-Exs, on markers, with focus influence Results Fibrosis peaked between 4th 5th weeks. Both TGFβ inhibitor EW-7197 reduced edema fibrosis, having most significant effect skin fibrosis. evident decreased levels TGFβ1, Smad2/3, phosphorylated along increased Smad7 levels, indicating that effectively pathway reduce Conclusions Our findings demonstrate significantly alleviate mouse model. largely mediated regulation pathway, suggesting promising approach for SLE.

Language: Английский

Exosomes and their derivatives as biomarkers and therapeutic delivery agents for cardiovascular diseases: Situations and challenges DOI Creative Commons
Yunyang Xu,

Weimin Wan,

Huixuan Zeng

et al.

Journal of Translational Internal Medicine, Journal Year: 2023, Volume and Issue: 11(4), P. 341 - 354

Published: Dec. 1, 2023

Microvesicles known as exosomes have a diameter of 40 to 160 nm and are derived from small endosomal membranes. Exosomes attracted increasing attention over the past ten years in part because they functional vehicles that can deliver variety lipids, proteins, nucleic acids target cells encounter. Because this function, may be used for diagnosis, prognosis treatment many diseases. All throughout world, cardiovascular diseases (CVDs) continue significant cause death. mediators communication between cells, which contribute physiological pathological aspects, aid improving CVD therapies biomarkers diagnosing predicting CVDs. Many studies demonstrated associated with CVDs, such coronary artery disease, heart failure, cardiomyopathy atrial fibrillation. participate progression or inhibition these mainly through contents deliver. However, application diferent CVDs is not very mature. So further research needed field.

Language: Английский

Citations

27
Pulmonary arterial hypertension nanotherapeutics: New pharmacological targets and drug delivery strategies DOI
Hamza Boucetta, Lei Zhang, Alejandro Sosnik

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 365, P. 236 - 258

Published: Nov. 24, 2023

Language: Английский

Citations

12
E-cigarette-induced changes in cell stress and mitochondrial function DOI Creative Commons
Chitteti Ramamurthy, Juan Pablo Zúñiga Hertz, Jorge A. Masso-Silva

et al.

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Chymase in Plasma and Urine Extracellular Vesicles: Novel Biomarkers for Primary Hypertension DOI Open Access
Sarfaraz Ahmad, Gagan Deep, Henry Punzi

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 10, 2023

ABSTRACT BACKGROUND Extracellular vesicles (EVs) have emerged as a promising liquid biopsy for various diseases. For the first time, using plasma and urinary EVs, we assessed activity of renin-angiotensin system (RAS), central regulator renal, cardiac, vascular physiology, in patients with control (Group I) or uncontrolled II) primary hypertension. METHODS EVs were isolated from 34 history hypertension, characterized size concentration by nanoparticle tracking analyses, exosomal biomarkers immunogold labeling coupled transmission electron microscopy, flow cytometry immunoblotting. analyzed hydrolytic chymase, angiotensin converting enzyme (ACE), ACE2, neprilysin (NEP) HPLC. RESULTS Plasma enriched small expressed markers (CD63, CD9, CD81). The (but not EVs) was significantly larger Group II compared to I. Differential RAS enzymes observed, higher chymase ACE, NEP EVs. Similarly, exhibited ACE ACE2 activity. Importantly, I, observed (p = 0.03) II, while no significant difference other enzymes. CONCLUSIONS Bioactive are present Detecting uncovers novel mechanism II-forming could also mediate cell-cell communication modulate signaling pathways recipient cells. GRAPHICAL

Language: Английский

Citations

4
miR‐30d Attenuates Pulmonary Arterial Hypertension via Targeting MTDH and PDE5A and Modulates the Beneficial Effect of Sildenafil DOI Creative Commons

Xuchun Liang,

Jingwen Zhou, Hongyun Wang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(40)

Published: Aug. 29, 2024

Pulmonary arterial hypertension (PAH) is associated with aberrant pulmonary vascular smooth muscle cell (PASMC) function and remodeling. MiR-30d plays an important role in the pathogenesis of several cardiovascular disorders. However, miR-30d PAH progression remained unknown. Our study shows that circulating level significantly reduced plasma from patients. In transgenic (TG) rats, overexpressing attenuates monocrotaline (MCT)-induced (PH) Increasing also inhibits platelet-derived growth factor-bb (PDGF-bb)-induced proliferation migration human PASMC. Metadherin (MTDH) phosphodiesterase 5A (PDE5A) are identified as direct target genes miR-30d. Meanwhile, nuclear respiratory factor 1 (NRF1) acts a positive upstream regulator Using knockout (KO) rats treated sildenafil, PDE5A inhibitor used clinical therapies, it further found suppressing partially beneficial effect sildenafil against MCT-induced PH The present protective remodeling through targeting MTDH reveals modulates treating PAH. should be prospective to treat

Language: Английский

Citations

1
The Grifola frondosa-derived ACE inhibitory peptide attenuated the exosomes-mediated phenotype transformation of vascular smooth muscle cells DOI Creative Commons
Tianyuan Song, Tiantian Zhang,

Qiaolin Cai

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 122, P. 106502 - 106502

Published: Oct. 8, 2024

Language: Английский

Citations

1
Brain-derived extracellular vesicles as serologic markers of brain injury following cardiac arrest: A pilot feasibility study DOI Creative Commons

Hanfei Shen,

Daria Zaitseva, Zijian Yang

et al.

Resuscitation, Journal Year: 2023, Volume and Issue: 191, P. 109937 - 109937

Published: Aug. 15, 2023

Language: Английский

Citations

3
Extracellular vesicles of Weizmannia coagulans lilac-01 reduced cell death of primary microglia and increased mitochondrial content in dermal fibroblasts in vitro DOI

Kimiko Minamida,

Toshio Taira,

Masato Sasaki

et al.

Bioscience Biotechnology and Biochemistry, Journal Year: 2023, Volume and Issue: 88(3), P. 333 - 343

Published: Dec. 20, 2023

ABSTRACT We investigated the properties of extracellular vesicles from probiotic Weizmannia coagulans lilac-01 (Lilac-01EVs). The phospholipids in Lilac-01EV membrane were phosphatidylglycerol and mitochondria-specific cardiolipin. found that applying Lilac-01EVs to primary rat microglia vitro resulted a reduction microglial cell death (P < .05). Lilac-01EVs, which contain cardiolipin phosphatidylglycerol, may have potential inhibit microglia. addition senescent human dermal fibroblasts suggested Lilac-01 EVs increase mitochondrial content without affecting their these cells.

Language: Английский

Citations

2
Exosomes from Hypoxia Preconditioned Muscle‐Derived Stem Cells Enhance Cell‐Free Corpus Cavernosa Angiogenesis and Reproductive Function Recovery DOI

Tianwen Peng,

Muyuan Chai, Zhicong Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(28)

Published: July 15, 2024

Tissue engineering for penile corpora cavernosa defects requires microvascular system reconstruction.GelMA hydrogels show promise tissue regeneration. However, using stem cells faces challenges such as immune rejection, limited proliferation and differentiation, biosafety concerns. Therefore, acellular regeneration may avoid these issues. Exosomes are used from muscle-derived (MDSCs) to modify 3D-printed hydrogel scaffolds Hypoxia-preconditioned MDSC-derived exosomes obtained enhance the therapeutic effect. In contrast normoxic (N-Exos), hypoxic (H-Exos) found markedly proliferation, migration, capillary-like tube formation of human umbilical vein endothelial (HUVECs). High-throughput sequencing analysis miRNAs isolated both N-Exos H-Exos revealed a significant upregulation miR-21-5p in following preconditioning. Further validation demonstrated that miR-21-5p/PDCD4 pathway promoted HUVECs. Epigallocatechin gallate (EGCG) is introduced improve mechanical properties biocompatibility GelMA hydrogels. EGCG-GelMA loaded with different types Exos transplanted repair rabbit defects, observed blood flow status defect site through color Doppler ultrasound magnetic resonance imaging, ultimately restored erection function successfully bred offspring. Thus, offer an effective treatment defects.

Language: Английский

Citations

0
Extracellular Vesicles and Cardiovascular Disease: Diagnostic and Therapeutic Perspective DOI
Vivek P. Chavda, Pankti C. Balar, Dixa A. Vaghela

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0