Paternal Cyanidin-3-O-Glucoside Diet Improved High-Fat, High-Fructose Diet-Induced Intergenerational Inheritance in Male Offspring's Susceptibility to High-Fat Diet-Induced Testicular and Sperm Damage

Reproductive Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

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Transposition element MERVL regulates DNA demethylation through TET3 in oxidative-damaged mouse preimplantation embryos

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: March 12, 2025

Abstract Transposable elements (TEs) comprise approximately half of eukaryotic genomes and significantly contribute to genome plasticity. In this study, we focused on a specific TE, MERVL, which exhibits particular expression during the 2-cell stage commonly serves as an indicator embryonic totipotency. However, its precise role in embryo development remains mysterious. We utilized DRUG-seq investigate effects oxidative damage genes TEs expression. Our findings revealed that exposure hydrogen peroxide (H 2 O ) could induce DNA damage, apoptosis, incomplete demethylation embryos, were potentially associated with MERVL To further explore function, antisense nucleotides (ASO) targeting constructed knockdown early embryos. Notably, led occurrence apoptosis stage, consequently reducing number embryos progress blastocyst stage. Moreover, discovered exerted influence reprogramming methylation. MERVL-deficient activity demethylase ten-eleven translocation 3 (TET3) was suppressed, resulting impaired when compared normal development. This impairment might underpin mechanism impacts Collectively, our study not only verified crucial but also probed regulatory function methylation reprogramming, thereby laying solid foundation for investigations into MERVL's role.

Language: Английский

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Applying an evolutionary perspective to assisted reproductive technologies

PNAS Nexus, Journal Year: 2024, Volume and Issue: 3(12)

Published: Nov. 28, 2024

Abstract Assisted reproductive technologies (ART) are commonly used to address human infertility and boost livestock production. During ART, procedures such as in vitro fertilization, artificial insemination, intracytoplasmic sperm injection introduce gametes embryos unnatural potentially stressful conditions that can influence offspring health, often via epigenetic effects. In this perspective we summarize these key risks of ART for embryonic longer-term fitness, emphasizing the need experimental research on animal models determine causal links between fitness across multiple generations. We also highlight how bypass a range naturally sexually selected mechanisms occur female tract and/or secretions ultimately which fertilize their eggs. further argue curtailment female-modulated selection may have important consequences ART-conceived offspring. encourage development methods better mimic natural processes embrace fundamental principles sexual selection. Ultimately, aim is dialogue fields evolutionary biology applied areas reproduction.

Language: Английский

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DI George Syndrome in Newborns Conceived From Assisted Reproductive Techniques: Is Preimplantation Screening Necessary? A Discussion of Two Cases

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 26, 2024

Background: Assisted reproductive techniques are increasingly used, and thus the use of tests for identification possible genetic errors in embryo should be optimized, which translate to occurrence congenital diseases newborns. DiGeorge syndrome (DGS) is a severe that implies higher risk complex heart metabolic immunological abnormalities. Reports DGS newborns conceived via intracytoplasmic sperm injection (ICSI) limited only two cases, one involved diagnosis implanted embryo, resulting pregnancy termination. We present cases pregnancies ICSI, without prenatal with disease. Case presentation: Case 1 involves full-term female newborn, structural anomalies. At birth, patient presented hypotonia, hypocalcemia, seizures, phenotypic characteristics DGS; she also had disease characterized by interruption aortic arch. A successful surgical repair was performed, required oral calcium supplementation anticonvulsants upon hospital discharge. Case 2 preterm male ICSI surrogate uterus, born cyanosis, retrognathia, short neck, other minor He diagnosed Tetralogy Fallot hypocalcemia; correction performed at 6 months age, he supplementation. Both follow-ups year complications, confirmed DGS. Conclusions: associated anatomical abnormalities can potentially compromise life including Its association assisted limited. It necessary consider preimplantation screening when there suspected increased DGS, especially family history this syndrome. Preimplantation considered based on individual gestational carrier, father, findings anomalies

Language: Английский

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Comparing subjective indicators of health in adults aged 27-38 years, conceived with and without assisted reproductive technology

Reproductive BioMedicine Online, Journal Year: 2024, Volume and Issue: unknown, P. 104777 - 104777

Published: Dec. 1, 2024

Language: Английский

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