PM2.5 exposure reprograms cell cycle dynamics in uterine immune cells at single-cell resolution DOI Creative Commons
Lin Zhang, Jiaqi Tian, Yong‐Fei Zheng

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Fine particulate matter (PM2.5) exposure has been associated with adverse effects on reproduction, yet the underlying cellular mechanisms remain poorly understood. Using single-cell RNA sequencing, we systematically investigated cell cycle dynamics of immune populations in mouse uterus following PM2.5 exposure. Analysis 9,000 balanced cells was performed to identify distinct and characterize changes distribution gene expression profiles. induced alterations composition distributions. Notably, observed significant populations, including reductions macrophages (510 58 cells), NK (445 91 granulocytes (1597 1 cells). Cell analysis demonstrated type-specific responses exposure: showed increased G1 phase representation (53.45%, +7.37%) decreased G2M (18.97%, -12.79%), while exhibited relatively modest (G1: 28.6%, +2.5%; G2M: 45.1%, +2.6%; S: 26.4%, -5.1%). Differential further identified crucial regulatory genes involved control, Cd81 Nrp1 macrophages, Vps37b cells. Integration markers differentially expressed revealed distinctive phase-specific perturbations across types. induces progression uterine cells, which provides novel mechanistic insights into environmental pollution-induced reproductive dysfunction.

Language: Английский

PM2.5 exposure reprograms cell cycle dynamics in uterine immune cells at single-cell resolution DOI Creative Commons
Lin Zhang, Jiaqi Tian, Yong‐Fei Zheng

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Fine particulate matter (PM2.5) exposure has been associated with adverse effects on reproduction, yet the underlying cellular mechanisms remain poorly understood. Using single-cell RNA sequencing, we systematically investigated cell cycle dynamics of immune populations in mouse uterus following PM2.5 exposure. Analysis 9,000 balanced cells was performed to identify distinct and characterize changes distribution gene expression profiles. induced alterations composition distributions. Notably, observed significant populations, including reductions macrophages (510 58 cells), NK (445 91 granulocytes (1597 1 cells). Cell analysis demonstrated type-specific responses exposure: showed increased G1 phase representation (53.45%, +7.37%) decreased G2M (18.97%, -12.79%), while exhibited relatively modest (G1: 28.6%, +2.5%; G2M: 45.1%, +2.6%; S: 26.4%, -5.1%). Differential further identified crucial regulatory genes involved control, Cd81 Nrp1 macrophages, Vps37b cells. Integration markers differentially expressed revealed distinctive phase-specific perturbations across types. induces progression uterine cells, which provides novel mechanistic insights into environmental pollution-induced reproductive dysfunction.

Language: Английский

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