Multi-omics analysis of two rat models reveals potential role of vesicle transport and autophagy in right ventricular remodeling DOI Creative Commons
Yuhan Qin, Jing Zhang, Aiwei Wang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Right ventricular failure as a severe consequence of pulmonary arterial hypertension (PAH) is an independent risk factor for poor prognosis, although the pathogenesis right remodeling (RVR) remains unclear. Exploring shared molecular pathways and key molecules in ventricle monocrotaline (MCT) artery banding (PAB) rat models may reveal critical RVR mechanisms. Untargeted proteome metabolome analysis were performed on myocardium two (MCT-induced PAH rats PAB-operated rats) to identify altered proteins metabolites, followed by validation using parallel reaction monitoring quantitative real-time polymerase chain (qPCR). The multi-omics profiles MCT PAB compared explore dysregulated RVR. Our proteomics study identified 25 RVR-altered differentially expressed proteins. Multiple common biological between models, encompassing myocardial energy metabolism alternation, etc. Various related vesicle transport autophagy identified, including nidogen-1, soluble N-ethylmaleimide-sensitive attachment protein receptors (SNAREs) signaling pathway, microautophagy pathway (all previously unreported RVR). Glycerophospholipid was sole statistically significant metabolic enriched metabolomics. Underreported processes, autophagy, contribute pathophysiology PAH-induced

Language: Английский

Multi-omics analysis of two rat models reveals potential role of vesicle transport and autophagy in right ventricular remodeling DOI Creative Commons
Yuhan Qin, Jing Zhang, Aiwei Wang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Right ventricular failure as a severe consequence of pulmonary arterial hypertension (PAH) is an independent risk factor for poor prognosis, although the pathogenesis right remodeling (RVR) remains unclear. Exploring shared molecular pathways and key molecules in ventricle monocrotaline (MCT) artery banding (PAB) rat models may reveal critical RVR mechanisms. Untargeted proteome metabolome analysis were performed on myocardium two (MCT-induced PAH rats PAB-operated rats) to identify altered proteins metabolites, followed by validation using parallel reaction monitoring quantitative real-time polymerase chain (qPCR). The multi-omics profiles MCT PAB compared explore dysregulated RVR. Our proteomics study identified 25 RVR-altered differentially expressed proteins. Multiple common biological between models, encompassing myocardial energy metabolism alternation, etc. Various related vesicle transport autophagy identified, including nidogen-1, soluble N-ethylmaleimide-sensitive attachment protein receptors (SNAREs) signaling pathway, microautophagy pathway (all previously unreported RVR). Glycerophospholipid was sole statistically significant metabolic enriched metabolomics. Underreported processes, autophagy, contribute pathophysiology PAH-induced

Language: Английский

Citations

0