Adult sex change leads to extensive forebrain reorganization in clownfish DOI Creative Commons
Coltan G. Parker, George W. Gruenhagen,

Brianna E. Hegarty

et al.

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 23, 2024

Abstract Background Sexual differentiation of the brain occurs in all major vertebrate lineages but is not well understood at a molecular and cellular level. Unlike most vertebrates, sex-changing fishes have remarkable ability to change reproductive sex during adulthood response social stimuli, offering unique opportunity understand mechanisms by which nervous system can initiate coordinate sexual differentiation. Methods This study explores forebrain using single nucleus RNA-sequencing anemonefish Amphiprion ocellaris , producing first atlas brain. Results We uncover extensive differences cell type-specific gene expression, relative proportions cells, baseline neuronal excitation, predicted inter-neuronal communication. Additionally, we identify cholecystokinin, galanin, estrogen systems as central axes Supported these findings, propose model conserved decision-making network spanning multiple subtypes neurons glia, including subpopulations within preoptic area that are positioned regulate gonadal Conclusions work deepens our understanding defines rich suite pathways differentiate adult anemonefish.

Language: Английский

Arginine Vasotocin Directly Regulates Spermatogenesis in Adult Zebrafish (Danio rerio) Testes DOI Open Access
Maya Zanardini, Weimin Zhang, Hamid R. Habibi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6564 - 6564

Published: June 14, 2024

The neuropeptide vasopressin is known for its regulation of osmotic balance in mammals. Arginine vasotocin (AVT) a non-mammalian homolog this that present fish. Limited information suggested and homologs may also influence reproductive function. In the study, we investigated direct effect AVT on spermatogenesis, using zebrafish as model organism. Results demonstrate receptors (avpr1aa, avpr2aa, avpr1ab, avpr2ab, avpr2l) are expressed brain testes. action spermatogenesis was an ex vivo culture mature testes 7 days. Using histological, morphometric, biochemical approaches, observed actions testicular treatment directly increased number spermatozoa androgen-dependent manner, while reducing mitotic cells proliferation activity type B spermatogonia. stimulatory spermiogenesis blocked by flutamide, androgen receptor antagonist. results support novel hypothesis stimulates short-term spermiogenesis. However, prolonged presence lead to diminished spermatogonia B, resulting turnover spermatogonia, spermatids, spermatozoa. overall findings offer insight into physiological significance vertebrates contributing factor multifactorial male reproduction.

Language: Английский

Citations

2
Understanding neuroendocrine regulatory pathways of sexual behavior in teleosts DOI Creative Commons

Saokat Ahamed,

Toshinobu Tokumoto

Aquaculture and Fisheries, Journal Year: 2023, Volume and Issue: 9(3), P. 447 - 454

Published: May 25, 2023

The main neuroendocrine pathways of induction reproductive behavior in fish are elucidated. 17α, 20β-dihydroxy-4-pregnen-3-one (17,20β-DHP), an oocyte maturation-inducing hormone, was found to act as a pheromone induce male behavior. 20β-DHP-sulfate (17,20β-DHP-S), derivative 17,20β-DHP, also identified later-acting pheromone. In addition, prostaglandin F2 alpha (PGF2α), which is synthesized during female ovulation, has been shown pheromone, inducing the final oviposition-inducing make fertilization successful. It appears that these molecular events initiated by stimuli from brain. Numerous neuropeptides have discovered promote initiation Recently, with proliferation genome editing technology, detailed analysis molecules involved underway. Analysis gene knockout zebrafish and medaka actively On other hand, many research reports adversely affected chemical substances. Knowledge adverse effects substances not only useful information prevent their mixing viewpoint protecting natural environment point caution farming, but provides clues for elucidating induction. elucidation important view breeding technology farming biodiversity conservation. this review, we summarize recent on possibility developing new aquaculture techniques depending results.

Language: Английский

Citations

4
Reproductive neuroendocrinology in teleost fishes DOI
Vance L. Trudeau, Katherine Shaw,

Victoria Spadacini

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 29 - 61

Published: Jan. 1, 2024

Language: Английский

Citations

1
Multi-omics analysis reveals the cerebral sex-specific responses to chronic hypoxia in yellow catfish (Pelteobagrus fulvidraco) DOI

Danqing Yin,

Cheng Zhao

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 54, P. 101401 - 101401

Published: Dec. 13, 2024

Language: Английский

Citations

1
Perinatal exposure to the fungicide ketoconazole alters hypothalamic control of puberty in female rats DOI Creative Commons
Delphine Franssen, Hanna Johansson, David López Rodríguez

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: April 3, 2023

Introduction Estrogenic endocrine disrupting chemicals (EDCs) such as diethylstilbestrol (DES) are known to alter the timing of puberty onset and reproductive function in females. Accumulating evidence suggests that steroid synthesis inhibitors ketoconazole (KTZ) or phthalates may also affect female health, however their mode action is poorly understood. Because hypothalamic activity very sensitive sex steroids, we aimed at determining whether how EDCs with different can transcriptome GnRH release rats. Design Female rats were exposed KTZ DES during perinatal (DES 3-6-12μg/kg.d; 3-6-12mg/kg.d), pubertal adult periods 3-12-48μg/kg.d; 3-12-48mg/kg.d). Results Ex vivo study pulsatility revealed exposure highest doses delayed maturation secretion before puberty, whereas had no effect on pulsatility. Hypothalamic transcriptome, studied by RNAsequencing preoptic area mediobasal hypothalamus, was found be all effects persisting until adulthood. Bioinformatic analysis Ingenuity Pathway Analysis predicted “Creb signaling Neurons” “IGF-1 signaling” among most downregulated pathways “PPARg” a common upstream regulator driving gene expression changes. Deeper screening ofRNAseq datasets indicated high number genes regulating extrinsic pulse generator consistently affected puberty. Several, including MKRN3, DNMT3 Cbx7, showed similar alterations Conclusion nRH highly both KTZ. The identified should exploredfurther identify biomarkers for future testing strategies EDC identification when enhancing current standard information requirements regulation.

Language: Английский

Citations

3
Neurohypophysial and paracrine vasopressinergic signaling regulates aquaporin trafficking to hydrate marine teleost oocytes DOI Creative Commons
Alba Ferré, François Chauvigné, Magdalena Gozdowska

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 11, 2023

The dual aquaporin (Aqp1ab1/Aqp1ab2)-mediated hydration of marine teleost eggs, which occurs during oocyte meiosis resumption (maturation), is considered a key adaptation underpinning their evolutionary success in the oceans. However, endocrine signals controlling this mechanism are almost unknown. Here, we investigated whether nonapeptides arginine vasopressin (Avp, formerly vasotocin) and oxytocin (Oxt, isotocin) involved using gilthead seabream (Sparus aurata) as model. We show that concomitant with an increased systemic production Avp Oxt, also produced accumulated locally ovarian follicles maturation hydration. Functional characterization representative Oxt receptor subtypes indicates Avpr1aa Oxtrb, expressed postvitellogenic oocyte, activate phospholipase C protein kinase pathways, while Avpr2aa, highly follicular theca granulosa cells, activates cAMP-protein A (PKA) cascade. Using ex vivo, vitro mutagenesis approaches, determined Avpr2aa plays major role PKA-mediated phosphorylation subdomains driving membrane insertion Aqp1ab2 Aqp1ab1 distal proximal regions microvilli, respectively. data further indicate luteinizing hormone, surges maturation, induces synthesis cells via progestin nuclear receptor. Collectively, our suggest both neurohypophysial paracrine vasopressinergic systems integrate to differentially regulate trafficking Aqp1ab-type paralogs common Avp-Avpr2aa-PKA pathway avoid competitive occupancy same plasma space maximize water influx

Language: Английский

Citations

2
Oxytocin: A developmental journey DOI Creative Commons
Karen L. Bales

Comprehensive Psychoneuroendocrinology, Journal Year: 2023, Volume and Issue: 16, P. 100203 - 100203

Published: Aug. 18, 2023

The neuropeptide hormone oxytocin is involved in many processes our bodies, linking social lives to internal states. I started out my career studying primate families, an interest that expanded into the role of family-oriented behaviors such as pair bonding and parenting prairie voles, humans, other primates. Starting a post-doc with Dr. C. Sue Carter, also became interested during development way we manipulate clinically. During then faculty member at University California, Davis, have worked on number these questions.

Language: Английский

Citations

2
Male-specific vasotocin expression in the medaka tuberal hypothalamus: Androgen dependence and probable role in aggression DOI Creative Commons
Yukika Kawabata-Sakata, Shinji Kanda, Kataaki Okubo

et al.

Molecular and Cellular Endocrinology, Journal Year: 2023, Volume and Issue: 580, P. 112101 - 112101

Published: Nov. 3, 2023

Language: Английский

Citations

2
Catecholamines modulate differentially nonapeptide precursor mRNA expression in the preoptic area and ovary of the catfish Heteropneustes fossilis: An in vitro study DOI
Putul Banerjee, Radha Chaube, K.P. Joy

et al.

General and Comparative Endocrinology, Journal Year: 2023, Volume and Issue: 333, P. 114211 - 114211

Published: Jan. 13, 2023

Language: Английский

Citations

1
Male-specific vasotocin expression in the medaka tuberal hypothalamus: androgen dependence and probable role in aggression DOI Open Access
Yukika Kawabata-Sakata, Shinji Kanda, Kataaki Okubo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 13, 2023

Abstract Terrestrial vertebrates have a population of androgen-dependent vasotocin (VT)-expressing neurons in the extended amygdala that are more abundant males and mediate male-typical social behaviors, including aggression. Teleosts lack these but instead novel male-specific VT-expressing tuberal hypothalamus. Here we found medaka vt expression is dependent on post-pubertal gonadal androgens can act to directly stimulate transcription via androgen receptor subtype Ara. Furthermore, administration exogenous VT induced aggression females alterations milieu led correlated changes levels hypothalamic both sexes. However, genetic ablation failed prevent androgen-induced females. Collectively, our results demonstrate marked dependence teleost hypothalamus, although its relevance needs be further validated.

Language: Английский

Citations

1