Omics analysis of the effect of cold normal saline stress through gastric gavage on LPS induced mice DOI Creative Commons
Jing Li, Zhihao Cui, Ming Wei

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 14, 2023

Cold stress is a significant environmental stimulus that negatively affects the health, production, and welfare of animals birds. However, specific effects cold stimulation combined with lipopolysaccharide (LPS) on mouse intestine remain poorly understood. Therefore, we designed this research to explore effect + LPS mice via microbiome microbiota sequencing. Forty-eight were randomly divided into four experimental groups ( n = 12): Control (CC), LPS-induced (CL), normal saline-induced (MC) (ML). Our results showed body weight was similar among different mice. in CC CL slightly higher compared those MC ML. The gene expressions reflected ML exposure caused gut injury barrier dysfunction, as evident by decreased ZO-1 , OCCLUDIN P < 0.01), CASPASE-1 0.01) expression Moreover, found induced oxidative LPS-challenged increasing malondialdehyde (MDA) accumulation decreasing antioxidant capacity [glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total (T-AOC)]. promoted inflammatory response increased IL-1β treated saline LPS. Whereas, sequencing revealed differential abundance phyla 24 genera groups. Metabolism analysis demonstrated presence 4,320 metabolites mice, 43 up-regulated 19 down-regulated vs. animals, well 1,046 428 animals. It Concluded enhances intestinal damage disrupting balance metabolites, while our findings contribute improving management practices livestock during seasons.

Language: Английский

Dysregulation of immune response in PCOS organ system DOI Creative Commons
Jingxuan Wang, Tailang Yin, Su Liu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 5, 2023

Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder affecting women, which can lead to infertility. Infertility, obesity, hirsutism, acne, and irregular menstruation are just a few of issues that PCOS be linked to. has complicated pathophysiology range clinical symptoms. Chronic low-grade inflammation one features PCOS. The inflammatory environment involves immune metabolic disturbances. Numerous organ systems across body, in addition female system, have been affected by pathogenic role immunological dysregulation recent years. Insulin resistance hyperandrogenism associated with cell dysfunction cytokine imbalance. More importantly, obesity also involved PCOS, leading an women Hormone, interactions contribute pathogenesis Hormone imbalance may development autoimmune diseases. aim this review summarize pathophysiological various patients provide new ideas for systemic treatment future.

Language: Английский

Citations

45
Effects of Yulin Tong Bu formula on modulating gut microbiota and fecal metabolite interactions in mice with polycystic ovary syndrome DOI Creative Commons

Ya-Nan Su,

Meijiao Wang,

Jun-Pu Yang

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 6, 2023

Background Polycystic ovarian syndrome (PCOS) is a common endocrine disorder characterized by hyperandrogenism, dysfunction and polycystic morphology. Gut microbiota dysbiosis metabolite are associated with PCOS clinical parameters. Yulin Tong Bu formula (YLTB), traditional Chinese medicine formula, has been recently indicated to be capable of ameliorating ovary symptoms correcting abnormal glucose metabolism. However, the therapeutic mechanism YLTB on not fully elucidated. Methods A pseudo sterile mouse model was established during this four-day acclimatization phase giving animals an antibiotic cocktail remove gut microbiota. Here, effects were investigated using dehydroepiandrosterone plus high-fat diet-induced mice model. Female prepuberal randomly divided into three groups; namely, control group, group (38.68 g·kg -1 ·day ) group. To test whether effect microbiota, we performed 16S rRNA sequencing studies analyze fecal mice. The relationships among metabolites, phenotypes further explored Spearman correlation analysis. Then, ferulic acid then validated in Results Our results showed that treatment ameliorated features (ovarian dysfunction, delayed clearance, decreased insulin sensitivity, deregulation glucolipid metabolism hormones, etc.) significantly attenuated dysbiosis. analysis metabolites such as folic negatively correlated similar YLTB. In addition, bacterial species Bacteroides dorei fragilis found positively related parameters, association study Conclusion These suggest systematically regulates interaction between ameliorate PCOS, providing solid theoretical basis for validation human trials.

Language: Английский

Citations

27
Effects of TCM on polycystic ovary syndrome and its cellular endocrine mechanism DOI Creative Commons
Huize Chen, Chujun Deng, Zeyu Meng

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: May 16, 2023

Polycystic ovary syndrome (PCOS) is a reproductive endocrine disease characterized by menstrual disorders, infertility, and obesity, often accompanied insulin resistance metabolic disorders. The pathogenesis of PCOS relatively complex has certain relationship with increase androgen luteinizing hormone (LH) the main cause series symptoms. Traditional Chinese medicine (TCM) obvious advantages significant curative effects in treatment this disease. It can effectively reduce level patients, regulate lipid metabolism, ovulation rate pregnancy fewer side effects. This article reviews efficacy safety herbs other TCM (such as acupuncture) its complications recent years, well effect mechanism on cellular endocrine, order to provide new clinical idea for PCOS.

Language: Английский

Citations

19
E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism DOI Creative Commons
Man Luo, Yuanyuan Chen,

Xiangyang Pan

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 19, 2023

Objective Gut microbiota and its metabolites have regulatory effects on PCOS related ovarian dysfunction insulin resistance. Escherichia coli Nissle 1917 (EcN) is a genetically controlled probiotic with an excellent human safety record for improving gut microbiome metabolic disorders immune system disorders. Here we focused to explore the application effect of EcN microbiota-metabolism-IL-22-mitochondrial damage axis in PCOS. Methods mice were constructed dehydroepiandrosterone (DHEA) treated EcN, FMT or IL-22 inhibitors. Clinically control subjects included further analysis. Serum follicular fluid supernatant levels sex hormones, insulin, glucose, cholesterol, inflammatory factors detected by ELISA biochemical reagents. The pathological changes tissues observed HE staining. JC-1 level COX4 gene expression granulosa cells was RT-qPCR. expressions progesterone receptor A (PR-A), LC3II/I, Beclin1, p62 CytC western blot. number autophagosomes electron microscopy. 16S rRNA LC-MS/MS used analyze metabolism. Results promoted recovery hormone tissue morphology, IL-22, PR-A cells, inhibited mitophagy mice. decreased microbiota, significantly increased abundance Adlercreutzia , Allobaculum Escherichia-Shigella Ileibacterium improved Amino sugar nucleotide metabolism pathways. positively associated Progesterone, negatively RF39 Luteinizing hormone, Testosterone, N−Acetylglucosamin, L−Fucose N−Acetylmannosamin. reconfirmed that ameliorated mitochondrial but this process blocked inhibitor. Clinical trials demonstrated reduced patients. Conclusion promoting inhibiting amount amino

Language: Английский

Citations

13
Traditional Chinese Medicine in Osteoporosis Intervention and the Related Regulatory Mechanism of Gut Microbiome DOI
Kun Li, Yiping Jiang, Nani Wang

et al.

The American Journal of Chinese Medicine, Journal Year: 2023, Volume and Issue: 51(08), P. 1957 - 1981

Published: Jan. 1, 2023

The gut microbiome (GM) has become a crucial factor that can affect the progression of osteoporosis. A number studies have demonstrated impact Traditional Chinese Medicine (TCM) on GM and bone metabolism. In this review, we summarize potential mechanisms relationship between osteoporosis disorder introduce several natural medicines exert anti-osteoporosis effects by modulating GM. It is underlined that, through provision microbial associated molecular pattern (MAMP), causes inflammatory reactions alterations in Treg–Th17 balance ultimately leads to changes mass. Serotonin many hormones, especially estrogen, may play role interaction with Additionally, absorption specific nutrients intestine, particularly minerals like calcium, magnesium, phosphorus. Several herbs, such as Sambucus Williamsii, Achyranthes bidentata Blume, Pleurotus ostreatus Ganoderma lucidum mushrooms, Pueraria Lobata, Agaricus blazei Murill exhibited regulating distribution metabolism These herbs increase abundance Firmicutes, decrease Bacteroides, promote produce more SCFAs, modulate immune response caused harmful bacteria, proportion indirectly Moreover, gut-derived 5-HT an important target for TCM prevent via gut-bone axis. Puerarin could improving intestinal mucosal integrity systemic inflammation estrogen deficiency.

Language: Английский

Citations

11
Gut microbiota: an emerging target connecting polycystic ovarian syndrome and insulin resistance DOI Creative Commons

Yufeng Mei,

Wanzhen Li, Bingqi Wang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 11, 2025

Polycystic ovary syndrome (PCOS) is a highly heterogeneous metabolic disorder, with oligomenorrhea and hirsutism as patients’ primary complaints. Hyperinsulinemia crucial pathophysiological mechanism in the development of PCOS, 50-70% patients exhibiting insulin resistance (IR). This condition not only exacerbates ovulatory dysfunction but also leads to various adverse outcomes, such dyslipidemia diabetes, increases risk cardiovascular events both before after menopause. Gut microbiota microbial community within host that possesses significant potential shaped by external environmental factors, neuro-immune network, metabolism. Recent studies have shown gut dysbiosis closely related progression PCOS. Despite growing recognition role pathogenesis treatment its clinical application remains infancy. Currently, most guidelines expert consensus still emphasize traditional therapeutic approaches, hormonal treatments, lifestyle modifications, sensitizers. However, accumulating evidence suggests may influence reproductive health PCOS through mechanisms. Therefore, understanding between IR essential. review describes changes IR-PCOS patients, examines mechanisms which contributes updates supporting regulatory target IR-PCOS. In summary, be involved improving offer stability benefits.

Language: Английский

Citations

0
Polycystic ovary syndrome: Insights into its prevalence, diagnosis, and management with special reference to gut microbial dysbiosis DOI
Nisha H. Khobragade, Devang Sheth, Chirag Patel

et al.

Steroids, Journal Year: 2024, Volume and Issue: 208, P. 109455 - 109455

Published: June 13, 2024

Language: Английский

Citations

3
Systematic low-grade chronic inflammation and intrinsic mechanisms in polycystic ovary syndrome DOI Creative Commons
Hongxia Deng, Yan Chen, Jiadi Xing

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 19, 2024

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder affecting 6-20% of women childbearing age worldwide. Immune cell imbalance dysregulation inflammatory factors can lead to systematic low-grade chronic inflammation (SLCI), which plays pivotal role in the pathogenesis PCOS. A significant higher infiltration immune cells such as macrophages lymphocytes pro-inflammatory IL-6 TNF-α has been detected PCOS organ systems, impacting not only female reproductive system but also other organs cardiovascular, intestine, liver, thyroid, brain organs. Obesity, insulin resistance (IR), steroid hormones intestinal microecological imbalance, deficiencies vitamin D selenium, well hyperhomocysteinemia (HHcy) induce between anti-inflammatory molecules. The cytokines interact with obesity, IR, leading increased reproductive-endocrine dysfunction patients. This review aims summarize response intrinsic mechanisms SLCI provide new insights for systemic treatment future.

Language: Английский

Citations

2
Trends in metabolic dysfunction in polycystic ovary syndrome: a bibliometric analysis DOI Creative Commons
Yan Xu,

Zhiqun Cao,

Ting Chen

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 28, 2023

Polycystic ovary syndrome (PCOS) is a very common chronic disease and causes reproductive disorders in women of childbearing age worldwide. The cause metabolic dysfunction PCOS unknown, there lack systematic bibliometric analysis for this disease. This study included 3,972 articles on published from 2012 to 2021. We applied the VOSviewer CiteSpace scientometric software analyze data regarding publication articles, countries, authors, institutions, scientific categories, cited journals, keywords. Through analysis, we determined research efforts their developing trends anticipated progress understanding PCOS-related dysfunction.

Language: Английский

Citations

6
Signaling pathways and targeted therapeutic strategies for polycystic ovary syndrome DOI Creative Commons
Kexin Wang, Yànhuá Lǐ

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 19, 2023

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. Although promising strides have been made in field PCOS over past decades, distinct etiologies this are not fully elucidated. Prenatal factors, genetic variation, epigenetic mechanisms, unhealthy lifestyles, and environmental toxins all contribute to development intricate highly heterogeneous metabolic, endocrine, reproductive, psychological disorder. Moreover, interactions between androgen excess, insulin resistance, disruption hypothalamic–pituitary–ovary (HPO) axis, obesity only make for a more complex picture. In review, we investigate summarize related molecular mechanisms underlying pathogenesis from perspective level signaling pathways, including PI3K/Akt, TGF-β/Smads, Wnt/β-catenin, Hippo/YAP. Additionally, review provides an overview prospective therapies, such as exosome therapy, gene drugs based on traditional Chinese medicine (TCM) natural compounds. By targeting these aberrant interventions primarily alleviate inflammation, ovarian fibrosis, which typical symptoms PCOS. Overall, hope that paper will pave way better understanding management future.

Language: Английский

Citations

6