Gut microbiota-metabolite interactions meditate the effect of dietary patterns on precocious puberty

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109887 - 109887

Published: May 3, 2024

Precocious puberty, a pediatric endocrine disorder classified as central precocious puberty (CPP) or peripheral (PPP), is influenced by diet, gut microbiota, and metabolites, but the specific mechanisms remain unclear. Our study found that increased alpha-diversity abundance of short-chain fatty acid-producing bacteria led to elevated levels luteinizing hormone follicle-stimulating hormone, contributing puberty. The integration microbiota metabolites has potential diagnostic value for

Language: Английский

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Organic Metal Chalcogenides-Assisted Metabolic Molecular Diagnosis of Central Precocious Puberty

Chemical Science, Journal Year: 2023, Volume and Issue: 15(1), P. 278 - 284

Published: Nov. 27, 2023

Cu(SPh–COOH) was precisely designed and for the first time investigated as a LDI-MS substrate with enhanced energy transfer ionization efficiency. A high-accuracy metabolic molecular diagnosis of CPP achieved using Cu(SPh–COOH)-assisted platform.

Language: Английский

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Integrated analysis of proteomics and metabolomics in infantile epileptic spasms syndrome

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 6, 2025

Infantile Epileptic Spasms Syndrome (IESS) is a severe developmental epileptic encephalopathy that manifests in infancy, significantly impacting the health and quality of life affected children. The treatment IESS poses significant challenge, primarily due to incomplete understanding its etiology pathogenesis. Objective: This study aims investigate pathogenic mechanisms IESS, utilizing metabolomics proteomics analyses uncover potential biomarkers for disease, thereby providing new insights diagnostic therapeutic strategies. Cerebrospinal fluid samples from 6 patients control subjects with benign intracranial hypertension were collected analyzed using techniques. Significant differential metabolites proteins identified correlated determine key associated specific metabolites. then expanded sample size 10 per group validated through ELISA analysis. A total 24 (12 upregulated 12 downregulated) 79 (18 61 identified. Metabolomic analysis suggests linoleic acid highly noteworthy metabolite cerebrospinal patients. protein HLA-A SEZ6L2 notably downregulated (p < 0.05). Linoleic metabolism-related could serve as into complex disease. Additionally, these findings also assist identifying targets developing more effective

Language: Английский

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Serum Metabolomic Analysis of Healthy and Central Precocious Puberty Girls

Clinical Endocrinology, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

The incidence of precocious puberty (PP) has been on the rise in recent years. Based different control mechanisms, childhood PP is divided into central (CPP) and peripheral (PPP). CPP accounts for 80% all cases. Metabolomics considered a link between genomics phenotypes, providing direct reflection intricate biological traits. However, studies serum metabolomic changes are very limited. In this study, non-targeted metabolomics analysis from healthy controls groups was performed. Serum samples were collected total 55 individuals, including 30 girls diagnosed with who had not yet received treatment did have any other comorbidities, 25 serving as underwent physical examinations. A 1107 differential metabolites identified, 681 upregulated 426 downregulated ones. main pathway involved citrate cycle (TCA cycle), primary bile acid biosynthesis, arginine purine metabolism, caffeine alanine, aspartate glutamate valine, leucine isoleucine beta-alanine taurine hypotaurine inositol phosphate sphingolipid pyruvate propanoate butanoate C5-branched dibasic sulphur carbon metabolism biosynthesis amino acids. 14 identified through combined four major metabolic network analyses. above form that may serve novel marker potential therapeutic target diagnosis CPP.

Language: Английский

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Multi-omic analysis of precocious puberty girls: pathway changes and metabolite validation

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 29, 2024

Objective Precocious puberty (PP) is a prevalent endocrine disorder affecting the physical and mental wellbeing of children. Identifying triggering factors PP has become central issue. This study seeks to investigate metabolomic transcriptomic alterations in PP. Material methods First, 37 school-aged girls diagnosed with 25 age-matched prepubertal control were recruited, fecal samples collected for non-targeted analysis screen differentially expressed metabolites (DEMs). Subsequently, an animal model was constructed by danazol administration neonatal female rats, both metabolomics serum next-generation sequencing performed DEMs genes (DEGs) Moreover, DEM co-existing clinical models administrated rats explore role target metabolite Results A total 24 180 425 DEGs identified. The Kyoto Encyclopedia Genes Genomes (KEGG) pathway showed that these enriched disease-associated pathways, including fatty acid synthesis, glycerolipid metabolism, pyrimidine steroid hormone biosynthesis, progesterone-mediated oocyte maturation, gonadotropin-releasing (GnRH) signaling pathway, forming tight DEM–DEG regulatory network. Further validation demonstrated thymine supplementation delayed opening vagina development rats. Conclusion reveals changes, along are implicated based on analyses. findings may provide new strategies research avenues treatment.

Language: Английский

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Lipidomic Analysis of Serum Lipid Profiles in Idiopathic Central Precocious Puberty and the Potential Regulatory Role of GnRHa in Lipid Metabolism

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(4)

Published: March 5, 2025

Idiopathic central precocious puberty (ICPP) is an endocrine disorder increasingly observed in children, commonly treated with gonadotropin-releasing hormone agonists (GnRHa). However, the serum lipid profiles girls ICPP and potential regulatory effects of GnRHa metabolism remain unclear. This research analyzed lipidomic samples from younger than 10 years age, including 37 patients ICPP, 32 GnRHa, 30 age-matched healthy controls. Using UHPLC-Q-Exactive Orbitrap/MS, we identified a total 898 differential lipids both positive negative modes, covering classes such as phosphatidylcholine (PC, EtherPC), acylcarnitine (CAR), triglyceride (TG), N-acyl ethanolamines (NAEs), diacylglycerol (DG), sphingomyelin (SM), phosphatidylethanolamine (PE), ceramide (Cer), fatty acids (FAs). In cohort, 105 exhibited significant differences compared controls, increased levels CAR, SM, PC, PE, TG, FA (p < 0.05), notably decreased NAE 0.05). Specifically, 20:1 (AUC: 1.0), 18:2 0.948), 20:0 0.895) were diagnostic biomarkers for ICPP. Following treatment, peak LH FSH decreased, alongside reversible changes 44 lipids, predominantly NAE, TG. conclusion, this study demonstrates that associated alterations metabolism. The induced by therapy suggest link between treatment homeostasis, which warrants further investigation to refine therapeutic approaches potentially mitigate metabolic disorders.

Language: Английский

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Comprehensive analysis of untargeted metabolomics and lipidomics in girls with central precocious puberty

Frontiers in Pediatrics, Journal Year: 2023, Volume and Issue: 11

Published: May 12, 2023

Objective Central precocious puberty (CPP) is a rare condition that causes early sexual development in children. Although the cure effective, etiology of central unclear. Methods In total, 10 girls with and same number age-matched female controls were enrolled. Plasma samples collected from each participant subjected to untargeted metabolomics lipidomics. Student's t -tests employed compare mean metabolite lipid. Furthermore, orthogonal partial least-squares discriminant analysis was conducted variable importance projection calculated identify differentially expressed metabolites or lipids. Subsequent bioinformatics investigate potential function Results Fifty-nine identified based on criteria used (variable &gt;1 P value &lt; 0.05). Kyoto Encyclopedia Genes Genome (KEGG) enrichment showed enriched four pathways: beta-alanine metabolism, histidine bile secretion, steroid hormone biosynthesis. As for lipidomics, 41 lipids observed chain length lipid saturation yielded similar results. Significant differences between two groups only (O-acyl) ω-hydroxy fatty acids (OAHFA). Conclusion The present study antibiotic overuse, increased meat consumption, obesity may have roles girls. Several diagnostic but further research required.

Language: Английский

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Lipidomics reveals ceramide biomarkers for detecting central precocious puberty in girls

Obesity Research & Clinical Practice, Journal Year: 2024, Volume and Issue: 18(4), P. 269 - 279

Published: July 1, 2024

Language: Английский

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Metabolomics analysis reveals that Qingxiangyin improves central precocious puberty through 13S-hydroxyoctadecadienoic acid mediated linoleic acid metabolic for regulating the PPAR pathways

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Journal Year: 2024, Volume and Issue: 52(11), P. 100454 - 100454

Published: Nov. 1, 2024

Language: Английский

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Association of size for gestational age and dehydroepiandrosterone sulfate with cardiometabolic risk in central precocious puberty girls

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: May 24, 2023

Objective The aim of this study was to assess whether size for gestational age and dehydroepiandrosterone sulfate (DHEAS) are associated with cardiometabolic risk in central precocious puberty (CPP) girls. Methods retrospective included 443 patients newly diagnosed CPP. Subjects were categorized by birth weight (appropriate [AGA], small [SGA], large [LGA] age) serum DHEAS concentration (high [≥75th percentile] normal [&lt;75th DHEAS). Cardiometabolic parameters examined. Composite (CMR) score calculated based on BMI, blood pressure, glucose, insulin, triglyceride, HDL cholesterol. Non-obesity CMR computed, omitting the value from BMI. Logistic regression models, general linear partial correlation analyses used evaluate associations. Propensity matching performed sensitivity analyses. Results Overall, 309 (69.8%) born AGA, 80 (18.1%) SGA, 54 (12.2%) LGA. Compared AGA counterparts, CPP girls SGA more prone have elevated HbA1c (adjusted OR = 4.54; 95% CI, 1.43–14.42) low cholesterol 2.33; 1.18–4.61). In contrast, being LGA not increased any glucose or lipid derangements. Despite fact that common among individuals than 1.84; 1.07–4.35), no significant difference found non-obesity 0.75; 0.30–1.88). When controlling age, SDS, current BMI-SDS, high exhibited higher apolipoprotein A-1 concentrations lower triglyceride level score. Furthermore, correlated positively negatively prominently after adjustments three abovementioned confounders. Sensitivity corroborated findings. Conclusion Among girls, those likely possess factors compared their peers. we observed between driven High favorable profile even subjects SGA.

Language: Английский

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