Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 14, 2023

Abstract The combination of genome editing and primordial germ cell (PGC) transplantation has enormous significance in the study developmental biology genetic breeding, despite its low efficiency due to limited number donor PGCs. Here, we employ a germplasm factors convert blastoderm cells into induced PGCs (iPGCs) zebrafish obtain functional gametes either through iPGC or via single blastomere overexpression factors. Zebrafish-derived blastula Gobiocypris rarus iPGCs, spermatozoa can be produced by iPGC-transplanted zebrafish. Moreover, knock-in perfectly resolves contradiction between high early lethality during embryonic stages greatly improves knock-in. Together, present an efficient method for generating teleost, technique that will have strong impact basic research aquaculture.

Language: Английский

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Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 58(1), P. 194 - 206

Published: Dec. 19, 2023

Bis(2-ethylhexyl)tetrabromophthalate (TBPH) has been widely detected in the environment and organisms; thus, its toxic effects on male reproduction were systematically studied. First, we found that TBPH can stably bind to androgen receptor (AR) based silico molecular docking results observed an antagonistic activity, but not agonistic AR signaling pathway using a constructed AR-GRIP1 yeast assay. Subsequently, validated adverse germ cells by observing inhibited production proliferation Leydig upon vitro exposure affected general motility motive tracks of zebrafish sperm ex vivo exposure. Finally, reproductive toxicity was demonstrated reduced mating behavior F0 generation when paired with unexposed females abnormal development their offspring. In addition, impaired also observed, which may be related disturbed homeostasis sex hormones. Notably, specifically suppressed brain provides further evidence for as above-mentioned. These confirmed through classical nuclear receptor-mediated pathway, would helpful assessing ecological health risks TBPH.

Language: Английский

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Biosynthetic deficiency of docosahexaenoic acid causes nonalcoholic fatty liver disease and ferroptosis-mediated hepatocyte injury

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(7), P. 107405 - 107405

Published: May 22, 2024

Language: Английский

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Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

Biology, Journal Year: 2023, Volume and Issue: 12(9), P. 1175 - 1175

Published: Aug. 28, 2023

The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis. Recapitulation of spermatogenesis in vitro hindered the challenge collecting under long-term culture conditions. Coilia nasus a commercially valuable anadromous migrant fish found Yangtze River China. In past few decades, exploitation deteriorating ecological environment have nearly caused extinction C. nasus's natural resources. present study, we established stable cell line (CnSSC) from gonadal tissue endangered species nasus. continued proliferate maintain morphology, normal diploid karyotype, gene expression patterns after more than one year (>80 passages). Additionally, CnSSC could successfully differentiate into sperm coculture system. Therefore, establishment lines model for studying feasible way preserve germplasm

Language: Английский

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<i>foxl2l</i> is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish

动物学研究, Journal Year: 2024, Volume and Issue: 45(5), P. 1116 - 1130

Published: Jan. 1, 2024

Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit polygenic sex determination system, where most males initially develop juvenile ovaries before committing male fate. In species with chromosomal determination, gonadal somatic cells are recognized key determinants Notably, the loss in leads masculinization, implying that harbor an intrinsic feminization signal. However, specific signal triggering oogenesis remains unclear. present study, we identified

Language: Английский

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Embryonic Exposure to the Benzotriazole Ultraviolet Stabilizer 2‐(2H‐benzotriazol‐2‐yl)‐4‐methylphenol Decreases Fertility of Adult Zebrafish (Danio rerio)

Environmental Toxicology and Chemistry, Journal Year: 2023, Volume and Issue: 43(2), P. 385 - 397

Published: Nov. 17, 2023

Benzotriazole ultraviolet stabilizers (BUVSs) are emerging contaminants of concern. They added to a variety products, including building materials, personal care paints, and plastics, prevent degradation caused by (UV) light. Despite widespread occurrence in aquatic environments, little is known regarding the effects BUVSs on organisms. The aim present study was characterize exposure 2-(2H-benzotriazol-2-yl)-4-methylphenol (UV-P) reproductive success zebrafish (Danio rerio) following embryonic exposure. Embryos were exposed, use microinjection, UV-P at <1.5 (control), 2.77, 24.25 ng/g egg, reared until sexual maturity, when performance assessed, which molecular biochemical endpoints analyzed. Exposure did not have significant effect fecundity. However, there fertilization success. Using UV-P-exposed males females, fertility decreased 8.75% low treatment group 15.02% high relative control. In reproduction assay with control 11.47% Embryonic might perturbed male sex steroid synthesis as indicated small changes blood plasma concentrations 17β-estradiol 11-ketotestosterone, statistically nonsignificant decreases mRNA abundances cyp19a1a, cyp11c1, hsd17b3. addition, transcript genes involved spermatogenesis, such nanos2 dazl, observed. Decreases later stages sperm development observed, suggesting that impaired spematogenesis, resulting quantity. first demonstrate latent BUVSs, specifically fish reproduction. Environ Toxicol Chem 2024;43:385-397. © 2023 Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Language: Английский

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Super-fast breeding of all-female giant grass carp by tiny zebrafish

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 8, 2024

Grass carp (Ctenopharyngodon idellus), a prominent species in freshwater aquaculture, boasts one of the largest body sizes and global production yields. However, reproduction genetic breeding grass has been significantly hindered, primarily due to its protracted sexual maturation period four five years need for extensive cultivation space. In this study, we develop super-fast strategy breed all-female within half year, leveraging surrogate small-sized laboratory fish, zebrafish (Danio rerio). We identify isolate female germline stem cells (GSCs) from juvenile at three months post-fertilization (mpf). The GSCs with XX chromosomes are transplanted into germ cell-depleted larvae days (dpf). undergo rapid spermatogenesis recipient. At after transplantation, all recipients males capable producing all-X sperm derived donor carp. When these fertilized wildtype eggs, population is generated. This groundbreaking achievement highlights feasibility large-sized fish small fishes, opens new avenue advancing aquaculture.

Language: Английский

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Super-fast generation of all-female grass carp via transplantation of female germline stem cell into zebrafish

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

Surrogate reproduction has emerged as a powerful biotechnology in fish breeding, mainly aiming at shortening the maturation period of aquaculture species. Grass carp (Ctenopharyngodon idellus), possesses one largest body sizes and highest global production yields freshwater aquaculture. However, genetic breeding grass are significantly hindered, primarily due to its protracted sexual nearly five years need for extensive cultivation space. In this study, we develop super-fast strategy breed all-female within half year, leveraging surrogate small-sized laboratory fish, zebrafish (Danio rerio). We characterized purified female germline stem cells (GSCs) from juvenile ovary three months post-fertilization, which is first report GSCs an The were transplanted into germ cell-depleted larvae generate zebrafish. underwent accelerated spermatogenesis recipients. Three after transplantation, recipients developed males capable producing all-X sperm derived donor GSCs. When these fertilized with wildtype eggs, population was produced. Our study demonstrates that XX chromosomes can be differentiated functional short time under control gonadal somatic niche, opens new avenue precision

Language: Английский

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Smoothened mediates medaka spermatogonia proliferation via Gli1–Rgcc–Cdk1 axis

Biology of Reproduction, Journal Year: 2023, Volume and Issue: 109(5), P. 772 - 784

Published: Aug. 8, 2023

Abstract The proliferation of spermatogonia directly affects spermatogenesis and male fertility, but its underlying molecular mechanisms are poorly understood. In this study, Smoothened (Smo), the central transducer Hedgehog signaling pathway, was characterized in medaka (Oryzias latipes), role were investigated. Smo highly expressed spermatogonia. ex vivo testicular organ culture a spermatogonial cell line (SG3) derived from mature testis, activation promoted proliferation, while inhibition induced apoptosis. expression glioma-associated oncogene homolog 1 (gli1) regulator cycle (rgcc) significantly upregulated SG3 after activation. Furthermore, Gli1 transcriptionally rgcc, Rgcc overexpression rescued apoptosis caused by or inhibition. Co-immunoprecipitation assay indicated that could interact with cyclin-dependent kinase (Cdk1) to regulate Collectively, our study firstly reveals mediates through Gli1–Rgcc–Cdk1 axis. addition, necessary survival This deepens understanding at level, provides insights into fertility control reproductive disease treatment.

Language: Английский

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Germ cells do not progress through spermatogenesis in the infertile zebrafish testis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 5, 2023

Vertebrate spermatogonial stem cells maintain sperm production over the lifetime of an animal but fertility declines with age. While morphological studies have greatly informed our understanding typical spermatogenesis, molecular and cellular mechanisms underlying spermatogenesis are not yet understood, particularly respect to onset fertility. We used single-cell RNA sequencing generate a developmental atlas zebrafish testis. Using 5 timepoints across adult life zebrafish, we described profiles in testis during after all germ cell stages detected testes from fertile older infertile males only contained spermatogonia reduced population spermatocytes. These remaining transcriptionally distinct spermatogonia. Immune including macrophages lymphocytes drastically increase abundance testes. Our reveals changes as ages defines roadmap for regulation male

Language: Английский

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