Frequency, morbidity and equity — the case for increased research on male fertility

Nature Reviews Urology, Journal Year: 2023, Volume and Issue: 21(2), P. 102 - 124

Published: Oct. 12, 2023

Language: Английский

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Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 110(6), P. 1135 - 1156

Published: April 19, 2024

Abstract Conventional, brightfield-microscopic semen analysis provides important baseline information about sperm quality of an individual; however, it falls short identifying subtle subcellular and molecular defects in cohorts “bad,” defective human animal spermatozoa with seemingly normal phenotypes. To bridge this gap, is desirable to increase the precision andrological evaluation humans livestock animals by pursuing advanced biomarker-based imaging methods. This review, spiced up occasional classic movie references but seriously scholastic at same time, focuses mainly on biomarkers altered male germ cell proteostasis resulting post-testicular carryovers proteins associated ubiquitin-proteasome system. Also addressed are redox homeostasis, epididymal maturation, sperm–seminal plasma interactions, surface glycosylation. Zinc ion homeostasis-associated sperm-borne components, including elements neurodegenerative pathways such as Huntington Alzheimer disease, discussed. Such spectrum biomarkers, imaged highly specific vital fluorescent probes, lectins, antibodies, reveals both obvious chromatin, deoxyribonucleic acid, accessory structures head tail. Introduction next-generation image-based flow cytometry into research clinical andrology will soon enable incorporation machine deep learning algorithms end point developing simple, label-free methods for diagnostics high-throughput phenotyping economically animals.

Language: Английский

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Advanced Sperm Selection Techniques for Assisted Reproduction

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(7), P. 726 - 726

Published: July 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Language: Английский

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Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 11, 2024

Forecasted increases in the prevalence and severity of extreme weather events accompanying changes climatic behavior pose potential risk to reproductive capacity humans animals ecological agricultural significance. While several studies have revealed that heat stress induced by challenges such as testicular insulation can elicit a marked negative effect on male system, particularly production spermatozoa, less is known about immediate impact function following subchronic whole-body exposure elevated ambient temperature. To address this knowledge gap, we exposed unrestrained mice conditions emulate wave (daily cycle 8 h at 35 °C followed 16 25 °C) for period 7 d. Neither testes or epididymides heat-exposed exhibited evidence gross histological change, similarly, spermatozoa males retained their functionality ability support embryonic development. However, embryos generated from experienced pronounced gene expression linked acceleration early embryo development, aberrant blastocyst hatching, increased fetal:placental weight ratio. Such were causally associated with an altered sperm small noncoding RNA (sncRNA) profile, these developmental phenotypes recapitulated microinjection wild-type sired control RNAs extracted spermatozoa. data highlight even relatively modest excursions temperature affect identify sncRNA profile particular point vulnerability imposed environmental stress.

Language: Английский

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Resolving Secondary Infertility: A Case Report of the Successful Treatment of Sperm DNA Fragmentation Through the Physiological Intracytoplasmic Sperm Injection Method

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

This case report presents an illustrative account of a couple experiencing secondary infertility attributed to the fragmentation DNA in sperm. Secondary infertility, inability conceive after having previously successfully conceived child, can be due various factors, including male issues. Sperm (SDF) has emerged as major factor influencing fertility, resulting poor embryo development and lower pregnancy rates. is about use advanced assisted reproductive technologies, specifically physiological intracytoplasmic sperm injection (PICSI) (ICSI), treat caused by DNA. PICSI enables identification selection spermatozoa with optimal functional integrity using hyaluronan, natural binding substance. Preparing dish requires skill precision. exhibiting high index were excluded from process enhance potential. The underwent controlled ovarian stimulation, egg retrieval, ICSI PICSI. treatment resulted successful conception singleton pregnancy. Subsequent prenatal monitoring indicated healthy progression, ultimately culminating delivery baby girl at term. highlights efficacy integrating method preceding ICSI, cases related SDF. Further research larger-scale studies are warranted approve findings this establish broader applicability approach.

Language: Английский

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Combined sperm selection techniques to boost the success of in Vitro Fertilization (IVF) and Intra Cytoplasmic Sperm Injection (ICSI)

Research Journal of Applied Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 13 - 23

Published: Jan. 13, 2024

This study aimed to assess the effectiveness of combined methods Density gradient centrifugation and swim-up (D.G.C/S.U) zeta potential (D.G.C/Z) in semen samples treat sperm abnormality, decrease DNA fragmentation, choose one best for Teratozoospermia patients who have high Sperm Fragmentation (SDF) undergoing assisted reproduction.Method: 101 patients, all teratozoospermia with fragmentation visited fertility clinic.Semen features were examined using established criteria According Recent World Health Organization (WHO), DGC /Z DGC/ SU carried out on After that, evaluated by Chromatin Dispersion (SCD) testing was used WHO detect damage recently. Result:The first method showed statistically significant higher motility DGC/su (p<0.001), while other significant, lower fewer abnormalities DGC/Z (p<0.001).We discovered no connection between defective morphology damage.There is link damage, indicating that are more likely be normal DNA. Conclusion:According results current study, use DGC/swim-up procedures improved motility, morphology, integrity samples, which may increase probability a successful pregnancy.

Language: Английский

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Bull sperm cryopreservation: An overview on the current status and future perspectives

German Journal of Veterinary Research, Journal Year: 2024, Volume and Issue: 4(1), P. 9 - 22

Published: Jan. 1, 2024

Cryopreservation refers to freezing cells or tissues at extremely low temperatures, allowing them be stored for extended periods while maintaining viability. Cryopreserved bull semen has become an essential tool in cattle breeding programs and commercial production systems. This review provides a detailed analysis of the current methods challenges preserving sperm using cryopreservation. We explore effects cryopreservation on cells, role different cryoprotectants, as well progress made semen. It also highlights impact process morphology functionality, emphasizing importance optimizing techniques maintain fertility The article underscores significance technology genetics suggests future research enhance techniques.

Language: Английский

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First-line infertility treatment in normal or subnormal sperm: Interest of a simplified pre-IMSI test

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0307080 - e0307080

Published: July 15, 2024

In the field of male infertility, when sperm is normal/subnormal, a few "add-on" routine tests can complete basic semen examination.

Language: Английский

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The association of sperm BAX and BCL-2 gene expression with reproductive outcome in Oligoasthenoteratozoospermia cases undergoing intracytoplasmic sperm injection: A case-control study

International Journal of Reproductive BioMedicine (IJRM), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

The B-cell lymphoma 2 (

Language: Английский

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ICSI Versus IMSI

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

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