Identification of lipid metabolism-related genes in dapagliflozin treated rats with diabetic cardiomyopathy by bioinformatics

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Background Diabetic cardiomyopathy (DCM) is a heart disease caused by the metabolic disorders of glucose and lipids associated with diabetes, leading to failure death in diabetic patients. Dapagliflozin (DAPA) serves as treatment for managing blood levels individuals type 2 diabetes mellitus (DM). However, specific mechanisms which DAPA treats DCM are not yet fully understood. Methods Sprague-Dawley (SD) rats (n = 5/group) were randomly divided into control, model, intervention groups. Lipid metabolism-related genes (LMRGs) gotten from publicly available database. Differential expression analysis model vs. control samples was performed obtain differentially expressed (DEGs), result recorded DEGs-Model DEGs-Intervention. The intersection opposing trends between DEGs-Intervention considered candidate genes. Subsequently, LMRGs intersected acquire hub genes, analyzed each group samples. Then, mechanism action these investigated through functional enrichment analysis, gene set (GSEA), predictive m6A binding sites. Results Ultimately, 68 590 derive (Acsbg1 Etnppl). Acsbg1 significantly increase compared group. RT-qPCR results confirmed obviously higher group, while Etnppl lower compare groups While Functional analyses indicated that fatty acid metabolism. findings GSEA might affect occurrence progression lysosome. And located at UCAGG RNA secondary structure. Conclusion This study identified Etnppl) potential new focal points diagnosing treating DCM.

Language: Английский

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Yunvjian decoction mitigates hyperglycemia in rats induced by a high-fat diet and streptozotocin via reducing oxidative stress in pancreatic beta cells

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 327, P. 118045 - 118045

Published: March 11, 2024

Language: Английский

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Nutritional Provision of Iron Complexes by the Major Allergen Alt a 1 to Human Immune Cells Decreases Its Presentation

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 11934 - 11934

Published: July 25, 2023

Alternaria alternata is a common fungus strongly related with severe allergic asthma, 80% of affected individuals being sensitized solely to its major allergen Alt 1. Here, we assessed the function 1 as an innate defense protein binding micronutrients, such iron–quercetin complexes (FeQ2), and impact on antigen presentation in vitro. Binding FeQ2 was determined docking calculations. Recombinant generated, ability, well secondary quaternary structure, by UV-VIS, CD, DLS spectroscopy. Proteolytic functions were casein gelatine zymography. Uptake empty apo– or ligand-filled holoAlt human monocytic THP1 cells under presence dynamin clathrin-inhibitors, activation Arylhydrocarbon receptor (AhR) using reporter cellline AZ-AHR. Human PBMCs stimulated for phenotypic changes monocytes flow cytometry. bound tetramer calculated Kd values reaching pico-molar levels surpassing affinities quercetin alone factor 5000 tetramer. apoAlt but not holoAlta showed low enzymatic activity against hexamer gelatin trimer. holo–Alt occurred partly clathrin-dependent, decreasing labile iron facilitating quercetin-dependent AhR activation. In uptake significantly decreased surface expression costimulatory CD86, also HLADR, thereby reducing effective presentation. We show here first time that nutritional complexes, FeQ2, alters dampens immune response, supporting notion only becomes immunogenic deprivation.

Language: Английский

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A real-world observational study on the effect of Qingre Lishi decoction on glycemic profile using continuous glucose monitoring in obese type 2 diabetes adults

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: July 23, 2024

To observe the clinical efficacy and safety of Qingre Lishi decoction in treating newly diagnosed overweight obese patients with type 2 diabetes mellitus (T2DM) from an evidence-based medical perspective.

Language: Английский

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