Multi-pronged molecular insights into flavonoid-mediated inhibition of squalene epoxidase: a pathway to novel therapeutics

RSC Advances, Journal Year: 2025, Volume and Issue: 15(5), P. 3829 - 3848

Published: Jan. 1, 2025

Apigenin-7-O-glucoside, silibinin, and baicalin are potent squalene epoxidase inhibitors with promising therapeutic potential. Integrative in silico experimental studies pave the way for hypercholesterolemia antifungal therapies.

Language: Английский

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Sea buckthorn-nutritional composition, bioactivity, safety, and applications: A review

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 133, P. 106371 - 106371

Published: June 1, 2024

Language: Английский

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Comprehensive review of extraction, purification, structural characteristics, pharmacological activities, structure-activity relationship and application of seabuckthorn protein and peptides

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 294, P. 139447 - 139447

Published: Jan. 5, 2025

Language: Английский

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Exploring the Association Between Dietary Fruit Intake and Endometriosis: A Systematic Review and Meta-Analysis

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(4), P. 1246 - 1246

Published: Feb. 13, 2025

Background/Objectives: Endometriosis is a chronic gynecological disorder affecting up to 10% of women reproductive age. The etiology endometriosis remains unclear; however, there growing interest in identifying modifiable risk factors, particularly dietary influences. present study aims systematically evaluate the association between fruit consumption and incidence endometriosis. Methods: A comprehensive systematic search was conducted across PubMed, Embase, Web Science, Google Scholar for studies published from 1 January 1990 30 September 2024. Relevant data were extracted categorized, methodological quality included assessed using Joanna Briggs Institute (JBI) checklists. Additionally, meta-analyses performed STATA 18.0 compare daily weekly among with without Results: analysis six comprising 3689 1463 controls. meta-analysis revealed no significant (odds ratio (OR): 0.95; 95% confidence interval (CI): 0.90-1.01). Similarly, did not demonstrate link (OR 1.03, CI: 0.78-1.35). assessment publication bias Begg's Egger's tests, along contour-enhanced funnel plots, indicated absence both groups. Conclusions: This indicates that does significantly influence developing Additional research necessary examine preferred interventions populations affected by this condition.

Language: Английский

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Genetic diversity of Hippophae rhamnoides varieties with different fruit characteristics based on whole-genome sequencing

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 4, 2025

1IntroductionSea buckthorn (Hippophae rhamnoides L.) is a woody oil tree known for its fruits, which are rich source of bioactive compounds, including carotenoids and flavonoids (Ciesarova et al., 2020; Mihal 2023). In addition, the unique fatty acid composition fruit pulp oil, especially high content omega-7 monounsaturated palmitoleic acid, rare in plants, contributes to nutritional benefits products (Sola Marsinach Cuenca, 2019). this regard, sea used medicine, cosmetics, nutraceuticals (Gatlan Gutt, 2021; Guo 2022; Zuchowski, addition cultivation production, also ecological restoration due resistance extreme conditions (Ruan 2013).Sea mainly cultivated China (2.07 million ha), India (0.02 Romania Mongolia Russia (0.01 Pakistan ha) (Nybom Thus, 90% resources located (Singh, 2022). However, pioneer breeding was Russia, where selection H. ssp. mongolica Rousi started 1933 allowed development wide range high-yield varieties with high-quality fruits (Singh Zubarev, 2014). contrast, later, sinensis Varieties characterized by large yield, content, lower acidity compared varieties, better adapted abiotic biotic stressors Sea does not stand still, new improved being developed genetic data can contribute this. only few DNA markers potentially useful known. Markers were proposed distinguish Hippophae species subspecies, (Liu 2015; Liu 2016; 2018; Piao dioecious, attempts made develop identify sex, but these do always work genetically diverse material (Korekar 2012; Das 2017; Zhou Zeng 2024a; 2024b). associated (Ding 2016) genes involved flavonoid biosynthesis (Zhang 2024) identified. Several works performed search recent years, genome assemblies (with sizes 849, 730, 919 Mb) (Wu Yu Yang 2024), tibetana (957 1453 (Wang 2022b; Zhang gyantsensis (716 (Chen obtained. very limited number genotypes studied using whole-genome sequencing. Whole-genome sequencing 40 wild representatives 15 Chinese researchers (Yu Therefore, there lack genomic buckthorn. The aim present study fill gap performing set 55 Russian breeding, likely be significantly different from valuable traits. These expand knowledge diversity at level provide necessary technologies breeding.2Materials Methods2.1Plant MaterialTo cover 56 accessions representing L. formed: one replicate 54 (one each variety) two biological replicates variety Elizaveta (two trees). following characteristics considered: weight, flavor, shape, color differences origin (Table 1). Characteristics assessed according Kondrashov al. (Kondrashov 1999). Dormant shoots selected collected Federal Altai Scientific Center Agrobiotechnologies (Barnaul, Russia) April 2023. placed containers water room temperature ~22 °C week. When leaves appeared, they tubes, frozen liquid nitrogen, stored low-temperature freezer until extraction.2.2DNA ExtractionDNA extracted Magen HiPure Plant Mini Kit (Magen, Guangzhou, China). quality quantity evaluated NanoDrop 2000C (Thermo Fisher Scientific, Waltham, MA, USA), Qubit 4.0 Scientific), agarose gel electrophoresis (2% agarose).2.3Whole-Genome SequencingThe QIAseq FX Library UDI (Qiagen, Chatsworth, CA, USA) library preparation. Quantity libraries Scientific) Qsep1-Plus (Bi-Optic, New Taipei City, Taiwan). Genome on NovaSeq 6000 (Illumina, San Diego, read length 150 + bp.2.4Sequencing Data AnalysisThe obtained Illumina reads processed Trimmomatic 0.39 (TRAILING:28, SLIDINGWINDOW:4:17, MINLEN:40) (Bolger mapped annotated CNGB Nucleotide Sequence Archive (https://db.cngb.org/cnsa), project ID CNP0001846 2022), VAF (Variant Allele Frequencies) values calculated regions corresponding (exons introns) PPLine (Krasnov 2015). Genetic distances between clustered Ward's method (ward.D2) 2015).3Preliminary AnalysisA representative comprising (for Elizaveta, trees analyzed) formed collection Russia). had origins order maximize analyzed 1).Whole-genome least 23 Gbases raw accession, corresponded more than 25× coverage (raw deposited NCBI SRA, BioProject PRJNA1177110). After mapping reference genome, about 4 polymorphisms (lists Zenodo, https://zenodo.org/records/13999625). studying allelic variants specific genes, those that may traits, such as compounds other stressors. It worth noting significant part identified all indicating distinct genome. evaluate their relationships (Supplementary Table 1).To visualize based gene sequences, dendrogram constructed (Figure Cluster I most included KP-686 (Kyrgyz ecotype), Dunayskaya, Yantarnaya Yagoda, probably have accessions. same cluster 175-02-01, crossing position expected requires additional research.The remaining divided into four clusters. II Afina progenies variety, namely 625-08-01, 625-14-1, 762-14-1, 763-14-1. assumed participation quite breeding. 111-05-01, 258-03-01, 42-68-2, believed unrelated Afina, II, difficult explain genealogical point view.Cluster III clearly distinguished group Panteleevskaya lineages. level.Cluster IV 14 among clear first three clusters, still present. Novosibirsk isolated: Triumf, Zarnitsa, 681-09-01, 708-13-1, Triumf parental form 681-09-01 708-13-1. Ulala progeny 125-02-01 cluster. Two lineages IV: 22-02-2003 226-00-01. general, however, contained mixture varieties.Cluster V cluster, some observed. For example, Rosinka Sudarushka, entered common roots. Essel 218-03-06 genotype 89-72-6a interesting terms strong inheritance size. respect, it progenitor many V. exception Aureliya, IV. Other traced Lyubimaya clone seedless mutant Lyubimaya. closely related groups replicates) 2016-00-1, Chechek Ognivo, Chuyskaya Klavdiya 111-10-2, 114-13-1.In us basis 2022) reflected well relationship genotypes. research authors separate ones, we unable find any sets (NCBI PubMed, https://pubmed.ncbi.nlm.nih.gov/; Google Scholar, https://scholar.google.com; accessed October 28, 2024). Meanwhile, linkage an urgent need studies (Sharma, 2022).Data great value understanding extent has affected pool crop what patterns analyzing data. We long history. forms traits created breeders became progenitors over world so special value. evaluation important when selecting crosses.Recently, been increasing articles devoted beneficial properties 2022a; Chen 2023; Nybom Teng 2024; Xu genetics, relatively understudied Indeed, several transcriptome (Bansal Ye Gao Lyu A synthesis genes/microRNAs process Ding 2019; Arkhipov determinants remain unknown key define carotenoid shape flavor. context, sequences phenotype will allow associations marker-assisted buckthorn, increasingly practice agricultural plants (Xu Hasan Thudi Dmitriev Werner Mangal 2024).4ConclusionsH. whose health benefits. To date, This fact hinders identification limits efficiency study, platform, accession. Based data, genes. varieties. revealed mostly Our assessing effect evaluating crosses. combination information quantitative trait loci (QTL) nucleotides (QTN) further tests. determine characteristics. help promote rhamnoides. our benefit both basic applied

Language: Английский

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Endocrine dysfunction post-traumatic brain injury: challenges and therapeutic approaches

Acta Neurologica Belgica, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

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A prospective study of vaginal topical pretreatment of compound sea-buckthorn oil suppository in postmenopausal women prior to colposcopy

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Postmenopausal women are faced with difficulties in colposcopy, such as easy bleeding from epithelial atrophy, and thinning, atypical colposcopy images, unsatisfactory exposure, decreased sensitivity of colposcopy. We aimed to improve the satisfaction postmenopausal evaluate clinical efficacy compound sea-buckthorn oil suppository on topical vaginal pretreatment prior patients who underwent Obstetrics Gynecology Hospital Fudan University April 2023 July 2024 were randomly assigned three groups: (A) untreated control group, (B) (C) estrogen ointment group. Group B group C treated for 2 weeks before adverse reactions analyzed. A total 377 included this study, which 9 excluded results failing complete 2-week medication, 368 results, including 125 A, 121 B, 122 C. The high-risk HPV infection accounted 94.3% (347/368), HPV16 and/or HPV18 20.4% (75/368), other types 75.3% (277/368). abnormal cytology (ASCUS above) 47.6% (175/368). No significant differences observed age, type among groups. There 196 cases (53.3%) pathologies postoperative colposcopic biopsy, 42 high-grade cervical squamous intraepithelial lesions. After integrity epithelium, adequacy ratio rate acetic acid test iodine staining groups significantly improved compared (P < 0.001), there was no difference between incidence breast discomfort 5.6%, while dizziness headache 4%. In contrast, side effects reported statistically significant. Additionally, statistical local Compound suppositories helpful improving reduce blind biopsies. not inferior estrogen. Compared ointment, most cost-effective have fewer can be applied practice.

Language: Английский

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Modeling the potential distribution of Hippophae rhamnoides in China under current and future climate scenarios using the biomod2 model

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 4, 2025

Introduction Hippophae rhamnoides, a temperate species with transcontinental distribution spanning Eurasia, demonstrates preferential establishment in water-limited ecosystems (arid/semi-arid zones), particularly occupying high-elevation niches skeletal soils and high solar flux. This ecologically significant plant, prized for dual ecological provisioning economic services, shows biogeographic concentration China’s northern desertification belts, northwestern Loess Plateau, southwestern montane corridors. Studying the possible areas where H. rhamnoides may be found can offer scientific foundation protection sustainable management of its resources. Methods study utilized biomod2 software to assess an integrated model based on 312 points 23 environmental factors. Furthermore, modeling analysis was conducted examine how geographical changes over time under SSP245 scenario. Results The findings show that is primarily affected by three factors: annual mean temperature, temperature seasonality coldest quarter. Currently, predominantly distributed provinces Shanxi, Shaanxi, Gansu, Hebei, Yunnan, Xinjiang, Tibet, Sichuan, Qinghai, Ningxia. suitable habitat covers area 212.89×10⁴ km², which represents 22.15% total land area. Within this region, high, medium, low suitability make up 23.15%, 22.66%, 54.20% habitat, respectively. Discussion In future, centroid expected gradually shift northwest, trend increasing west decreasing east. aims provide in-depth exploration influence factors it from perspective. These results are important improving conservation, management, cultivation, propagation while also offering research other valuable plant species.

Language: Английский

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Chemopreventive and therapeutic effects of Hippophae rhamnoides L. fruit peels evaluated in preclinical models of breast carcinoma

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 30, 2025

Background Cancer remains a major global health challenge, necessitating innovative prevention and treatment approaches. Certain plants, adapted to specific environments, may exhibit bioactive properties with potential anticancer applications. Hypothesis Seaberry ( Hippophae rhamnoides L. ) fruit peels exert effects in breast carcinoma (BC) models through the additive or synergistic actions of their unique secondary metabolites. Methods H. peel extracts were analyzed using LC-DAD-MS LC-DAD techniques profile content carotenoids flavonoids, respectively. The preclinical study evaluated seaberry BC models: (1) syngeneic 4T1 mouse adenocarcinoma model (triple-negative), (2) rat chemically induced mammary carcinogenesis, (3) vitro studies MCF-7 (hormone receptor-positive) MDA-MB-231 (triple-negative) cell lines. Results analyses identified dominant metabolites, including isorhamnetin, quercetin glycosides, kaempferol catechin, zeaxanthin, lutein. In model, resulted significant, dose-dependent reduction tumor volume (43% 48% compared controls) decrease mitotic activity index. Serum cytokine analysis showed reductions IL-6, IL-10, TNF-α. chemopreventive high-dose improved cancer prognosis by reducing ratio poorly differentiated tumors increasing caspase-3 Bax expression while decreasing Ki-67 malondialdehyde levels. Both doses elevated Bax/Bcl-2 reduced stem markers CD44, EpCam, VEGF controls. Epigenetic revealed histone modifications (H4K16ac, H4K20me3) altered methylation tumor-suppressor genes (PITX2, RASSF1, PTEN, TIMP3). Microarray (758 miRNAs) beneficial changes nine oncogenic/tumor-suppressive miRNAs, miR-10a-5p, miR-322-5p, miR-450a-5p, miR-142-5p, miR-148b-3p, miR-1839-3p, miR-18a-5p, miR-1949, miR-347. , ethanolic extract conferred partial resistance cisplatin-induced cytotoxicity cells at IC 50 concentrations. Conclusion This rodent shows promising data but requires rigorous, long-term validation. Integrating plant-based nutraceuticals into oncology necessitates precise cancer-type profiling patient stratification for effective personalized treatments.

Language: Английский

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Phenolic compounds in sea buckthorn (Hippophae rhamnoides L.) and their health‐promoting activities: a review

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: 59(9), P. 6642 - 6658

Published: April 12, 2024

Summary Sea buckthorn (SB) is used in cuisines and herbal remedies due to its remarkable nutritional composition health‐enhancing properties. The phenolic compounds present SB plant include acids (gallic, p ‐coumaric, protocatechuic ferulic acids), flavonols (isorhamnetin, quercetin kaempferol), flavan‐3‐ols polymeric procyanidins. highest content of these was reported branches leaves than corresponding edible parts the plant. antioxidant properties are related different parts. studies flavonoids (isorhamnetin quercetin) as main with medicinal potential health‐promoting effects. review summarises information available recent on composition, content, flavonoid activity activities (anti‐oxidant, hepatoprotective, cardioprotective, anti‐inflammatory, anti‐diabetic obesity management) that can be curtailing various acute chronic maladies. projected a promising source bioactive constituents healthy foods for beneficial effects human health.

Language: Английский

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