Comorbidity mechanisms of atherosclerosis and hashimoto's thyroiditis: A multiscale gene expression analysis based on microarray and single-cell sequencing DOI Creative Commons
Yirong Ma,

Shuguang Wu,

Junyu Lai

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 20, 2024

Abstract Background: Atherosclerosis (AS) is a chronic vascular disease characterized by inflammation of the arterial wall and formation cholesterol plaques. Hashimoto's thyroiditis (HT) an autoimmune disorder marked destruction thyroid tissue. Although previous studies have identified common risk factors between AS HT, specific etiology pathogenic mechanisms underlying these associations remain unclear. Method: We obtained relevant datasets for HT from Gene Expression Omnibus (GEO). By employing Limma package, we pinpointed differentially expressed genes (DEGs) discerned co-expression modules linked to via Weighted Co-expression Network Analysis (WGCNA). elucidated gene functions regulatory networks across various biological scenarios through enrichment pathway analysis using Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). Core were Cytoscape software further validated with external datasets. also conducted immune infiltration on core utilizing CIBERSORT method. Single-cell was instrumental in uncovering diagnostic markers. Lastly, predicted potential drugs targeting DGIdb database. Results: Based differential WGCNA, 119 candidate within cohorts HT. KEGG GO analyses indicate that are significantly involved antigen processing presentation, along immune-inflammatory pathways. Two pivotal genes, PTPRC TYROBP, five algorithms cytoHubba plugin. Validation confirmed their substantial value Moreover, results Set Enrichment (GSEA) indicated enriched receptor interactions signaling Immune revealed strong association lymphocytes macrophages pathogenesis demonstrated predominant expression macrophages, monocytes, T cells, CMP. Conclusion: This study proposes aberrant response might represent shared mechanism The TYROBP as critical biomarkers therapeutic targets comorbid conditions. Furthermore, cells could serve promising future strategies.

Language: Английский

Exploring the comorbidity mechanisms between atherosclerosis and hashimoto’s thyroiditis based on microarray and single-cell sequencing analysis DOI Creative Commons
Yirong Ma,

Shuguang Wu,

Junyu Lai

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 13, 2025

Abstract Atherosclerosis (AS) is a chronic vascular disease characterized by inflammation of the arterial wall and formation cholesterol plaques. Hashimoto’s thyroiditis (HT) an autoimmune disorder marked destruction thyroid tissue. Although previous studies have identified common risk factors between AS HT, specific etiology pathogenic mechanisms underlying these associations remain unclear. We obtained relevant datasets for HT from Gene Expression Omnibus (GEO). By employing Limma package, we pinpointed differentially expressed genes (DEGs) discerned co-expression modules linked to via Weighted Co-expression Network Analysis (WGCNA). elucidated gene functions regulatory networks across various biological scenarios through enrichment pathway analysis using Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). Core were Cytoscape software further validated with external datasets. also conducted immune infiltration on core utilizing CIBERSORT method. Lastly, Single-cell was instrumental in uncovering diagnostic markers. Based differential WGCNA, 119 candidate within cohorts HT. KEGG GO analyses indicate that are significantly involved antigen processing presentation, along immune-inflammatory pathways. Two pivotal genes, PTPRC TYROBP, five algorithms cytoHubba plugin. Validation confirmed their substantial value Moreover, results Set Enrichment (GSEA) indicated enriched receptor interactions signaling Immune revealed strong association lymphocytes macrophages pathogenesis demonstrated predominant expression macrophages, monocytes, T cells Common Myeloid Progenitor (CMP). This study proposes aberrant response might represent shared mechanism The TYROBP as critical potential biomarkers therapeutic targets comorbid conditions. Furthermore, could serve promising future strategies.

Language: Английский

Citations

0
Mechanisms underlying the promotion of papillary thyroid carcinoma occurrence and progression by Hashimoto’s thyroiditis DOI Creative Commons
Xiaohui Xue, Deqi Wu, Hongwen Yao

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

Hashimoto’s thyroiditis (HT) and papillary thyroid carcinoma (PTC) co-occurrence raises significant questions regarding the immune microenvironment molecular mechanisms in tumor development. This review synthesizes recent literature to explore characteristics of PTC patients with HT, analyze how these influence disease onset, progression, treatment. We focused on immunological biological underlying interaction between HT PTC, particularly recruitment activation cells alterations key signaling pathways. Studies indicate that exhibits distinctive microenvironmental features, such as role regulatory T (Tregs), IFN-γ-mediated CXCR3A-CXCL10 axis, NF-κB pathway activation. Additionally, thyroid-stimulating hormone (TSH) stimulation, RET/PTC gene rearrangements, changes STAT6 DMBT1 expression levels also play roles Notably, while may increase risk concurrent tend have better prognoses. Future research should further elucidate complex interplay two diseases prevent transformation into offer more personalized treatment plans for patients, including considerations preoperative thyroidectomy lymph node dissection strategies, well postoperative TSH suppression therapy assessment. underscores importance a deeper understanding interactions offers new perspectives future directions therapeutic strategies.

Language: Английский

Citations

0
Hashimoto’s thyroiditis– What’s in a name? DOI

Mikhail Khachaturov,

Dimitrios G. Goulis,

Petros Perros

et al.

HORMONES, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Comorbidity mechanisms of atherosclerosis and hashimoto's thyroiditis: A multiscale gene expression analysis based on microarray and single-cell sequencing DOI Creative Commons
Yirong Ma,

Shuguang Wu,

Junyu Lai

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 20, 2024

Abstract Background: Atherosclerosis (AS) is a chronic vascular disease characterized by inflammation of the arterial wall and formation cholesterol plaques. Hashimoto's thyroiditis (HT) an autoimmune disorder marked destruction thyroid tissue. Although previous studies have identified common risk factors between AS HT, specific etiology pathogenic mechanisms underlying these associations remain unclear. Method: We obtained relevant datasets for HT from Gene Expression Omnibus (GEO). By employing Limma package, we pinpointed differentially expressed genes (DEGs) discerned co-expression modules linked to via Weighted Co-expression Network Analysis (WGCNA). elucidated gene functions regulatory networks across various biological scenarios through enrichment pathway analysis using Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). Core were Cytoscape software further validated with external datasets. also conducted immune infiltration on core utilizing CIBERSORT method. Single-cell was instrumental in uncovering diagnostic markers. Lastly, predicted potential drugs targeting DGIdb database. Results: Based differential WGCNA, 119 candidate within cohorts HT. KEGG GO analyses indicate that are significantly involved antigen processing presentation, along immune-inflammatory pathways. Two pivotal genes, PTPRC TYROBP, five algorithms cytoHubba plugin. Validation confirmed their substantial value Moreover, results Set Enrichment (GSEA) indicated enriched receptor interactions signaling Immune revealed strong association lymphocytes macrophages pathogenesis demonstrated predominant expression macrophages, monocytes, T cells, CMP. Conclusion: This study proposes aberrant response might represent shared mechanism The TYROBP as critical biomarkers therapeutic targets comorbid conditions. Furthermore, cells could serve promising future strategies.

Language: Английский

Citations

0