Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice DOI Creative Commons
Jiexiang Zhao, Kang Tang,

Gurong Jiang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Abstract The transition from fetal primordial germ cells (PGCs) to spermatogonia (SPG) is critical for male cell development; however, the detailed transcriptomic dynamics and regulation underlying this remain poorly understood. Here by interrogating comprehensive transcriptome atlas dataset of mouse gonadal development, we elucidated regulatory networks transition. Our single‐cell analysis revealed that PGCs SPG was characterized global hypertranscription. A total 315 highly active regulators were identified be potentially involved in transition, among which a non‐transcription factor (TF) regulator TAGLN2 validated essential spermatogonial stem (SSCs) maintenance differentiation. Metabolism profiling also dynamic changes metabolism‐related gene expression during PGC Furthermore, uncovered intricate cell–cell communication exerted potential functions hypertranscription collaborating with stage‐specific regulators. Collectively, our work extends understanding molecular mechanisms offering insights into recapitulation generation vitro.

Language: Английский

Role of NR5A2 in regulating sex differentiation, steroidogenesis, and gonadal development in Chlamys farreri DOI

Lianxue Han,

Jingjing Miao, Min Ding

et al.

The Journal of Steroid Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: 248, P. 106690 - 106690

Published: Feb. 4, 2025

Language: Английский

Citations

1
Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice DOI Creative Commons
Yingdong Liu,

Fengdan Hao,

Hao Liang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 28, 2025

Introduction Microplastics and nanoplastics are prevalent environmental contaminants. Recent reports indicate that polystyrene nanoparticles may adversely impact male reproductive health. This study aims to examine the effects of exposure on sperm metabolism development pre-implantation embryos. Methods In this study, C57BL/6 mice were orally gavage-administered (60 nm, 20 mg/kg/day) for 35 days assess their reproduction early embryonic development. Experiments included testicular transcriptome analysis, metabolomics, motility fertilization assays, ROS detection, RNA sequencing 2-cell embryos, revealing adverse embryo Results The results revealed oral gavage induced a pronounced immune-inflammatory response in tissue, reduced motility, significantly lowered rate. Notably, from treated exhibited substantial metabolic disruptions, affecting key pathways, including glycerophospholipid biosynthesis DNA repair. After fertilization, embryos at stage suffered damage apoptotic repair subsequently impairing Discussion conclusion, demonstrated administration affects function, resulting abnormalities alterations associated gene expression profiles. These findings offer essential scientific insights future research into sperm-mediated transgenerational

Language: Английский

Citations

1
Characterization of Metabolic Patterns in Mouse Spermatogenesis and Its Clinical Implications in Humans DOI Open Access
Jiachen Wang, Mengqi Chen, Ying Yao

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1001 - 1001

Published: Jan. 24, 2025

Spermatogenesis is a complex process requiring precisely controlled metabolic adaptations. Although the genetic and cellular aspects of spermatogenesis have been extensively studied, underlying mechanisms remain largely underexplored. In this study, we utilized STA-PUT technology to separate three key cell types involved in mouse spermatogenesis: pachytene spermatocytes (PAC), round spermatids (RS), elongated (ES). A comprehensive untargeted metabolomic analysis revealed significant changes during spermatogenesis, such as reduced methylation-related metabolites increased glycolytic intermediates TCA cycle ES. Moreover, differences between germ cells somatic (Leydig Sertoli cells) were highlighted, particularly steroidogenesis lipid metabolism. To investigate clinical relevance, analyzed human seminal plasma. Samples from individuals with azoospermia displayed abnormalities, including methionine, tryptophan, arginine, which play vital roles sperm development. Pathway enrichment disturbances metabolism nucleotide, amino acid, energy azoospermia, suggesting potential biomarkers male infertility. Our findings provide profile suggest that alterations may be contributors infertility, cases azoospermia.

Language: Английский

Citations

0
Gestational exposure to PNMC reduces offspring gamete numbers by disrupting oocyte meiosis and spermatogenesis DOI Creative Commons

Tian Ma,

Hui Yu, Xinyu Zhou

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 117980 - 117980

Published: March 1, 2025

3-Methyl-4-nitrophenol (PNMC) is a prevalent nitrophenolic endocrine disruptor found in pregnant women, with known effects on offspring growth and development. However, its impact fertility remains unexplored. This study investigates the of PNMC exposure during pregnancy underlying mechanisms. Our assessments revealed that reduced number follicles spermatozoa offspring, though it did not affect their quality. In male impaired spermatogenesis by reducing Sertoli cells spermatogonia. female disrupted first meiotic prophase (MPI) oocytes, leading to diplotene oocytes available for primordial follicle assembly. depletion reserve ultimately resulted subfertility. Specifically, hindered homologous recombination-mediated DNA double-strand break repair, triggering activation checkpoint MPI arrest. arrested progression oocytes. provide comprehensive evidence reproductive capacity, elucidating key pathways. These findings emphasize need stricter regulatory measures limit offer new insights into etiology idiopathic oligozoospermia diminished ovarian reserve.

Language: Английский

Citations

0
Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice DOI Creative Commons
Jiexiang Zhao, Kang Tang,

Gurong Jiang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Abstract The transition from fetal primordial germ cells (PGCs) to spermatogonia (SPG) is critical for male cell development; however, the detailed transcriptomic dynamics and regulation underlying this remain poorly understood. Here by interrogating comprehensive transcriptome atlas dataset of mouse gonadal development, we elucidated regulatory networks transition. Our single‐cell analysis revealed that PGCs SPG was characterized global hypertranscription. A total 315 highly active regulators were identified be potentially involved in transition, among which a non‐transcription factor (TF) regulator TAGLN2 validated essential spermatogonial stem (SSCs) maintenance differentiation. Metabolism profiling also dynamic changes metabolism‐related gene expression during PGC Furthermore, uncovered intricate cell–cell communication exerted potential functions hypertranscription collaborating with stage‐specific regulators. Collectively, our work extends understanding molecular mechanisms offering insights into recapitulation generation vitro.

Language: Английский

Citations

1