Integrative Analysis of Whole-Genome and Transcriptomic Data Reveals Novel Variants in Differentially Expressed Long Noncoding RNAs Associated with Asthenozoospermia

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(1), P. 4 - 4

Published: Jan. 14, 2025

Background/Objectives: Asthenozoospermia, characterized by reduced sperm motility, is a common cause of male infertility. Emerging evidence suggests that noncoding RNAs, particularly long RNAs (lncRNAs), play critical role in the regulation spermatogenesis and function. Coding regions have well-characterized established predictive value asthenozoospermia. However, this study was designed to complement previous findings provide more holistic understanding asthenozoospermia, time focusing on regions. This aimed identify prioritize variants differentially expressed (DE) lncRNAs found exclusively asthenozoospermic men, their impact lncRNA structure lncRNA-miRNA-mRNA interactions. Methods: Whole-genome sequencing (WGS) performed samples from normozoospermic men. Additionally, an RNA-seq dataset individuals analyzed DE lncRNAs. Bioinformatics analyses were conducted map unique lncRNAs, followed prioritization based predicted functional impact. The structural effects lncRNA-miRNA interactions assessed using computational tools. Gene ontology (GO) KEGG pathway employed investigate affected biological processes pathways. Results: We identified 4173 mapped 258 After prioritization, 5 affect structure, while 20 17 disrupt miRNA-lncRNA Enriched pathways included Wnt signaling, phosphatase binding, cell proliferation, all previously implicated reproductive health. Conclusions: identifies specific may Given limited research utilizing WGS explore infertility, our valuable insights foundation for future studies.

Language: Английский

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Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1420 - 1420

Published: Feb. 8, 2025

Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks during spermatogenesis. PRDM9 determines localization hotspots interacting with several protein complexes in mammals. The function not well understood spermatogenesis mice or yaks. In this study, we applied yeast two-hybrid assays combined next-generation sequencing techniques to screen complete set PRDM9-interacting proteins and explore its novel functions yak Our results showed that 267 were identified. gene ontology (GO) analysis revealed GO terms primarily associated spermatogenesis, positive regulation break repair via homologous recombination, RNA splicing, ubiquitin-dependent ERAD pathway, other biological processes. MKX PDCD5 verified be strongly expressed prophase I meiosis both mouse testes. localizations splicing genes including THOC5, DDX5, XRCC6 spermatocytes. Cattleyak hybrid offspring a domestic cow, male are sterile. expression was also examined sterile cattle. Among 58 detected genes, 55 downregulated cattleyak. conclusion, established interaction network, identified, which will further promote our understanding It provides new insights for study sterility.

Language: Английский

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Lathyrol inhibits the proliferation of renca cells by affecting the expression of TGF-β/smad pathway and then affecting the cell cycle

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Background Renal cell carcinoma (RCC) is a prevalent malignant tumor with high morbidity and mortality. The TGF-β/smad signaling pathway plays significant role in the development progression of RCC. Methods This study explored impact lathyrol on proliferation mice RCC renca cells by inhibiting arresting cycle. Bioinformatics analysis, culture experiments, animal experiments were conducted to detect effects activity, mRNA, protein expressions xenograft tumors, as well cycle proteins regulatory proteins. Results Lathyrol treatment showed positive correlation inhibitory effect proliferation. IC values 786-O comparable. In vitro, decreased mRNA TGF-β1, TGF-βR1, smad2, smad3, smad4, smad9, while increasing smad4. vivo, suppressed smad9 xenografts, cyclinD1, cyclinB1, cyclinA1, cyclinE1, CDK6, CDK4, CDK1, P16, P21, P27. Conclusion can repress expression key pathway, impede signal transduction, arrest cells, subsequently inhibit cells. Future studies are needed further explore mechanism treatment.

Language: Английский

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Ameliorating effects of Ascorbic acid and Ammi Visnaga seeds on cigarette and water- pipe smoking Cytotoxicity in the lung and heart ventricle

Brazilian Journal of Biology, Journal Year: 2025, Volume and Issue: 85

Published: Jan. 1, 2025

A current study investigated the effects of smoking (Cigarettes and Water-pipes) on lung cardiac ventricles by comparing cytotoxic with certain natural antioxidants. 56 male albino rats were randomly assigned to 7 groups (N = 8 per group). Group 1 was a negative control that exposed fresh air; 2 positive group most popular cigarette brands in Jordanian market (red LM cigarettes) for 30 consecutive days, one rat. Groups 3 4 treatment antioxidants (vitamin C, Ammi Visnagia seed extract, respectively). Rats 5 flavored water-pipe smoke resulting from complete burning 20 g coastal session day, 6-7 selected (Ascorbic acid vitamin extract). According immunohistochemistry investigations, exposure has been linked tissue consequences. Both types models induced expression p53 protein heart tissues. However, mildly expressed considerably lung. In conclusion, investigations conducted impact different levels, such as histological changes trachea, lung, ventricle, well its influence protein. The administration Visnaga extract Ascorbic exhibited protective against detrimental through all aforementioned methods.

Language: Английский

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