Transcriptome analyses reveal key features of mouse seminal vesicle during aging

Systems Biology in Reproductive Medicine, Journal Year: 2024, Volume and Issue: 70(1), P. 249 - 260

Published: Aug. 21, 2024

Despite the significant morphological changes that occur in seminal vesicles with aging, transcriptomic characteristics remain largely unexplored. To address this, we performed bulk RNA sequencing on vesicle samples from mice aged 3, 13, and 21 months to uncover alterations. Our findings reveal display cystic dilatation, epithelial hypoplasia, disordered muscle layers, fibrosis, reduced proliferation capability. A comparison between 3-month-old 21-month-old indicated leukocyte-mediated immunity leukocyte migration were most significantly upregulated biological processes among differentially expressed genes (DEGs). Notably, several DEGs associated "leukocyte migration," such as Vcam1, Cxcl13, Ccl8, exhibited an increasing trend protein expression at three different time points of mice. Additionally, identified multiple aging-associated DEGs, including P21 Tnfrsf1b. Two (Cd209f Ccl8) consistently across all six regions male reproductive glands (testis, epididymis, vesicle) datasets These analyses highlight enhanced state immune inflammatory response vesicles. This study represents first exploration overall transcriptome landscape a murine model natural offering new insights into mechanisms underlying aging-related dysfunction.

Language: Английский

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Testing for age- and sex- specific mitonuclear epistasis in Drosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

ABSTRACT The need for efficient ATP production is predicted to result in the evolution of cooperation between mitochondrial and nuclear encoded components electron transport system. Novel (i.e., mismatched) mitonuclear genotype combinations are therefore negative fitness consequences, which may become more prevalent with ageing. Such effects expected be prominent males, since maternal inheritance mitochondria lead accumulation male-harming mutations (the mother’s curse hypothesis). To test these predictions, we measured female male fertility traits using a genetically diverse panel 27 populations Drosophila melanogaster matched or experimentally mismatched genomes at different ages. We found no overall effect mismatch. In females, epistasis. limited evidence epistasis affecting old age, however, not direction predicted. Experimentally males sired offspring one comparison. Sex-specific advantages might arise if novel alleles compensate deleterious that have accumulated. If such compensatory increasing occur nature, they could represent possible counterforce curse.

Language: Английский

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End of the Season Blues: Senescence and Reproductive Trade-Offs in Male Scorpions

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 916 - 916

Published: Nov. 23, 2024

Seasonal reproductive dynamics and senescence have profound impacts on male fertility, yet these processes remain understudied in scorpions. This study investigates how parameters-such as testicular mass, sperm quantity, viability-change over the course of season

Language: Английский

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