Cardenolides in the defensive fluid of adult large milkweed bugs have differential potency on vertebrate and invertebrate predator Na+/K+¬–ATPases DOI Open Access
Paola Rubiano-Buitrago, Shrikant Pradhan, Alfonso Aceves‐Aparicio

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

The defences of aposematic animals are characterised by diversity and variability secondary metabolites. Here we examine the nature function chemical defence in large milkweed bugs, Oncopeltus fasciatus, testing hypothesis that different compounds have evolved response to enemies. We profiled quantified cardenolides sequestered bugs their defensive secretions bodies, measured inhibitory properties a subset isolated insect’s against Na+/K+—ATPase target site vertebrate invertebrate predators, using porcine data as reference. show highly concentrated coroglaucigenin (glucopyranosyl frugoside frugoside) toxic for both resistant sensitive whereas corotoxigenin calotropagenin varying degrees enzyme inhibition among various predators. Overall, O. fasciatus is well defended range enemies due differential effect these compounds´ sites. Our results suggest insect sequester predation pressure.

Language: Английский

Biotechnological approaches to the production of commercially important cardiac glycosides from Digitalis L. (foxglove) DOI
Şeyma Önlü,

Cennet Yaman

Studies in natural products chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 235 - 278

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cardenolides in the defensive fluid of adult large milkweed bugs have differential potency on vertebrate and invertebrate predator Na + /K + -ATPases DOI Creative Commons
Paola Rubiano-Buitrago,

S. Pradhan,

A. A. Aceves

et al.

Royal Society Open Science, Journal Year: 2024, Volume and Issue: 11(6)

Published: June 1, 2024

Aposematic animals rely on diverse secondary metabolites for defence. Various hypotheses, such as competition, life history and multifunctionality, have been posited to explain defence variability diversity. We investigate the compound selectivity hypothesis using large milkweed bugs, Oncopeltus fasciatus , determine if distinct cardenolides vary in toxicity different predators. quantify bug’s defensive secretions body tissues test individual compounds against predator target sites, Na + /K -ATPases, that are predicted differ sensitivity. Frugoside, gofruside, glucopyranosyl frugoside gofruside were dominant of insects, whereas two monoglycosidic cardenolides—frugoside gofruside—were most abundant fluid. These highly toxic (IC 50 < 1 μM) an invertebrate a sensitive vertebrate enzyme, comparison glucosylated compounds. Gofruside was weakest inhibitor putatively resistant predator. Glucopyranosyl calotropin, found only 60% also effective enzymes. Our results suggest sequestered by O. probably provide consistency protection range predators underscore need consider communities prey evolution.

Language: Английский

Citations

1
Substrate Specificity of ABCB Transporters Predicted by Docking Simulations Can Be Confirmed by Experimental Tests DOI Creative Commons

Mario Röpcke,

Sha Lu,

Charles Plate

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5272 - 5272

Published: Nov. 7, 2024

ATP-binding cassette (ABC) transporters, particularly those of subfamily B, are involved in cell detoxification, multidrug resistance, drug treatment pharmacodynamics, and also ecological adaptation. In this regard, ABCB transporters may play a decisive role the co-evolution between plants herbivores. Cardenolides, toxic steroid glycosides, secondary plant metabolites that defend against herbivores by targeting their sodium-potassium ATPase. Despite toxicity, several herbivorous insects such as large milkweed bug (

Language: Английский

Citations

0
Cardenolides in the defensive fluid of adult large milkweed bugs have differential potency on vertebrate and invertebrate predator Na+/K+¬–ATPases DOI Open Access
Paola Rubiano-Buitrago, Shrikant Pradhan, Alfonso Aceves‐Aparicio

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

The defences of aposematic animals are characterised by diversity and variability secondary metabolites. Here we examine the nature function chemical defence in large milkweed bugs, Oncopeltus fasciatus, testing hypothesis that different compounds have evolved response to enemies. We profiled quantified cardenolides sequestered bugs their defensive secretions bodies, measured inhibitory properties a subset isolated insect’s against Na+/K+—ATPase target site vertebrate invertebrate predators, using porcine data as reference. show highly concentrated coroglaucigenin (glucopyranosyl frugoside frugoside) toxic for both resistant sensitive whereas corotoxigenin calotropagenin varying degrees enzyme inhibition among various predators. Overall, O. fasciatus is well defended range enemies due differential effect these compounds´ sites. Our results suggest insect sequester predation pressure.

Language: Английский

Citations

0