Lignocellulolytic carbohydrate-active enzyme-encoding gene clusters in novelTrichoderma atrovirideandTrichoderma harzianumstrains: A BAC-guided transcriptome analysis DOI Creative Commons
Paulo Azevedo, Maria Augusta Crivelente Horta, Rafaela Rossi Rosolen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Abstract Lignocellulosic biomass is known as a challenging substrate for enzymatic hydrolysis, increasing the processing cost in biorefineries. In nature, filamentous fungi, including those of genus Trichoderma , naturally degrade lignocellulose by using an arsenal hydrolytic and oxidative enzymes that act synergistically with degradation. This work explored genome organization target genes atroviride harzianum are able to promote hydrolysis identifying regions enriched degradative enzyme-encoding genes, namely, clusters. We employed bacterial artificial chromosome (BAC) methodology specific genomic explore their genetic organization, proximal gene context, expression under conditions. Using this tool, it was possible inspect linear structure data hydrolytic-rich regions. The results offered perspective related carbohydrate metabolism, revealing novel positively regulated during cellulose degradation contributing elucidating differences among species. Highlights High-quality were selected closely species CAZyme targets. RNA-seq used quantify BAC expression. Relevant identified combining functional differential data. Novel targets future characterization via

Language: Английский

Trichoderma: Advent of Versatile Biocontrol Agent, Its Secrets and Insights into Mechanism of Biocontrol Potential DOI Open Access
Nazia Manzar, Abhijeet Shankar Kashyap, Ravi Shankar Goutam

et al.

Sustainability, Journal Year: 2022, Volume and Issue: 14(19), P. 12786 - 12786

Published: Oct. 7, 2022

Trichoderma is an important biocontrol agent for managing plant diseases. species are members of the fungal genus hyphomycetes, which widely distributed in soil. It can function as a well growth promoter. now frequently used biological control agents (BCAs) to combat wide range Major diseases have been successfully managed due their application. spp. being extensively researched order enhance its effectiveness top agent. The activation numerous regulatory mechanisms major factor ability manage Trichoderma-based methods include nutrient competition, mycoparasitism, synthesis antibiotic and hydrolytic enzymes, induced resistance. may synthesize variety secondary metabolites that inhibit activity GPCRs (G protein-coupled receptors) membrane-bound receptors sense transmit environmental inputs affect metabolism. Related intracellular signalling pathways also play role this process. Secondary produced by activate disease-fighting within plants protect against pathogens. β- Glucuronidase (GUS), green fluorescent protein (gfp), hygromycin B phosphotransferase (hygB), producing genes examples exogenous markers could be identify track specific isolates agro-ecosystems. More than sixty percent biofungicides on market derived from species. These fungi harmful developing Additionally, they solubilize nutrients boost bioremediate contaminants through mechanisms, including mycoparasitism antibiosis. Enzymes industry. This review article intends provide overview update (from 1975 2022) fungi, information key metabolites, genes, interactions with

Language: Английский

Citations

96
Mechanisms for plant growth promotion activated by Trichoderma in natural and managed terrestrial ecosystem DOI
Hexon Ángel Contreras‐Cornejo, Monika Schmoll, Blanca Alicia Esquivel-Ayala

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127621 - 127621

Published: Jan. 21, 2024

Language: Английский

Citations

36
MAPkinases regulate secondary metabolism, sexual development and light dependent cellulase regulation in Trichoderma reesei DOI Creative Commons
Miriam Schalamun,

Sabrina Beier,

Wolfgang Hinterdobler

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 2, 2023

The filamentous fungus Trichoderma reesei is a prolific producer of plant cell wall degrading enzymes, which are regulated in response to diverse environmental signals for optimal adaptation, but also produces wide array secondary metabolites. Available carbon source and light the strongest cues currently known impact secreted enzyme levels an interplay with regulation metabolism became increasingly obvious recent years. While cellulase already be modulated by different mitogen activated protein kinase (MAPK) pathways, relevance signal, transmitted this pathway other fungi as well, still unknown T. interconnections chemical communication under mating conditions. Here we show that MAPkinases differentially influence darkness Hog1 homologue TMK3, not TMK1 or TMK2 required chemotropic glucose reesei. Additionally, regulate production specific metabolites including trichodimerol bisorbibutenolid, bioactive compound cytostatic effect on cancer cells deterrent larvae, conditions facilitating mating, reflects defect communication. Strains lacking either become female sterile, indicating conservation role sexual fertility In summary, our findings substantiate previously detected interconnection well involvement dependent gene metabolite genes fungi.

Language: Английский

Citations

19
Trichoderma and Mycosynthesis of Metal Nanoparticles: Role of Their Secondary Metabolites DOI Creative Commons
G. Herrera‐Pérez, Laura E. Castellano, Claudia A. Ramírez-Valdespino

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(7), P. 443 - 443

Published: June 22, 2024

Nanocompounds are widely used in many fields such as environmental, medicine, or agriculture. Nowadays, these nanocompounds mainly synthesized by chemical methods, causing environmental pollution and potential health problems. Thus, microorganisms have been investigated nanoparticle green biosynthesizers. The main research is focused on the synthesis of nanoparticles (NPs) using algae, yeast, bacteria, fungi. Among them, fungi most used, due to their simple effective mycosynthesis. Fungi well other organisms involved NPs use secondary metabolites (SMs) mediate catalyze reactions produce metal (MNPs) being able act capping agents producing different physicochemical characteristics biological activities MNPs. various for mycosynthesis

Language: Английский

Citations

8
Mycorrhizosphere bacteria inhibit greenhouse gas emissions from microplastics contaminated soil by regulating soil enzyme activities and microbial community structure DOI
Zeeshan Khan, Tariq Shah, Ghulam Haider

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 356, P. 120673 - 120673

Published: March 19, 2024

Language: Английский

Citations

7
The Sordariomycetes: an expanding resource with Big Data for mining in evolutionary genomics and transcriptomics DOI Creative Commons

Zheng Wang,

Wonyong Kim, Yen‐Wen Wang

et al.

Frontiers in Fungal Biology, Journal Year: 2023, Volume and Issue: 4

Published: June 30, 2023

Advances in genomics and transcriptomics accompanying the rapid accumulation of omics data have provided new tools that transformed expanded traditional concepts model fungi. Evolutionary flourished with use classical newer fungal models facilitate study diverse topics encompassing biology development. Technological advances also created opportunity to obtain mine large datasets. One such continuously growing dataset is Sordariomycetes, which exhibit a richness species, ecological diversity, economic importance, profound research history on amenable models. Currently, 3,574 species this class been sequenced, comprising nearly one-third available ascomycete genomes. Among these genomes, multiple representatives genera

Language: Английский

Citations

12
Trichoderma hamatum and Its Benefits DOI Creative Commons

Rathna Silviya Lodi,

Chune Peng, Xiaodan Dong

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(10), P. 994 - 994

Published: Oct. 8, 2023

Trichoderma hamatum (Bonord.) Bainier (T. hamatum) belongs to Hypocreaceae family, genus. spp. are prominently known for their biocontrol activities and plant growth promotion. Hence, T. also possess several beneficial activities, such as antimicrobial activity, antioxidant insecticidal herbicidal promotion; in addition, it holds other properties, resistance dichlorodiphenyltrichloroethane (DDT) degradation of DDT by certain enzymes production polysaccharide-degrading enzymes. the current review discusses properties describes gaps that need be further considered future studies, hamatum’s potentiality against human pathogens and, contrast, its role an opportunistic pathogen. Moreover, there is a substantial study on antiviral activities.

Language: Английский

Citations

11
Genome and transcriptome analysis of the lignite-degrading Trichoderma cf. simile WF8 strain highlights potential degradation mechanisms DOI

Jinghua Yao,

Yajuan Chen,

Deyu Zhuo

et al.

International Biodeterioration & Biodegradation, Journal Year: 2025, Volume and Issue: 198, P. 105997 - 105997

Published: Jan. 30, 2025

Language: Английский

Citations

0
From Field to Fork: the benefits of Trichoderma spp. in food quality and safety DOI
Carlos García‐Latorre, Santiago Ruiz‐Moyano, Alicia Rodríguez

et al.

Current Opinion in Food Science, Journal Year: 2025, Volume and Issue: unknown, P. 101286 - 101286

Published: Feb. 1, 2025

Language: Английский

Citations

0
Biotechnological tools for genetic improvement of Trichoderma DOI Creative Commons
Liliana Villao-Uzho, Fernando Espinoza, Luis Galarza

et al.

Scientia Agropecuaria, Journal Year: 2024, Volume and Issue: 15(2), P. 213 - 223

Published: April 8, 2024

Trichoderma is a cosmopolitan fungus widely distributed around the world. The different uses of this beneficial are varied in several industries, like agriculture, textile, and paper, including pharmaceutical industry. genus has other mechanisms action, production enzymes secreted secondary metabolites used various industries. genomes species have been sequenced to identify for producing compounds. advancement multiple technologies allowed development transformation tools genetic improvement Trichoderma, thus increasing biomass, primary metabolites, enzymes. Therefore, modification aims increase compound strains. Characterization through gene expression analysis essential biotechnology applications. It helps counteract one most challenging problems agriculture today, climate change appearance pathogens that attack crops with high commercial food demand. In conclusion, review analyzes strategies improve strains genetically their applications agricultural, As recommendation future studies potential impact, optimization specific modifications recommended adaptability effectiveness combating emerging challenges especially those linked change. Investigating possible synergies between modified environmentally sustainable agricultural practices could contribute developing solutions crop protection yield improvement.

Language: Английский

Citations

2