Lignocellulolytic carbohydrate-active enzyme-encoding gene clusters in novelTrichoderma atrovirideandTrichoderma harzianumstrains: A BAC-guided transcriptome analysis DOI Creative Commons
Paulo Azevedo, Maria Augusta Crivelente Horta, Rafaela Rossi Rosolen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Abstract Lignocellulosic biomass is known as a challenging substrate for enzymatic hydrolysis, increasing the processing cost in biorefineries. In nature, filamentous fungi, including those of genus Trichoderma , naturally degrade lignocellulose by using an arsenal hydrolytic and oxidative enzymes that act synergistically with degradation. This work explored genome organization target genes atroviride harzianum are able to promote hydrolysis identifying regions enriched degradative enzyme-encoding genes, namely, clusters. We employed bacterial artificial chromosome (BAC) methodology specific genomic explore their genetic organization, proximal gene context, expression under conditions. Using this tool, it was possible inspect linear structure data hydrolytic-rich regions. The results offered perspective related carbohydrate metabolism, revealing novel positively regulated during cellulose degradation contributing elucidating differences among species. Highlights High-quality were selected closely species CAZyme targets. RNA-seq used quantify BAC expression. Relevant identified combining functional differential data. Novel targets future characterization via

Language: Английский

Microbial organic fertilizer prepared by co-composting of Trichoderma dregs mitigates dissemination of resistance, virulence genes, and bacterial pathogens in soil and rhizosphere DOI
Bin Huang, Xiaolin Lv, Hao Zheng

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 241, P. 117718 - 117718

Published: Nov. 21, 2023

Language: Английский

Citations

4
Biological control of Corynespora leaf fall disease in rubber by endophytic Trichoderma spp. under field conditions DOI

Namphet Seekham,

Niphon Kaewsalong,

Arom Jantasorn

et al.

European Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: 170(2), P. 207 - 218

Published: May 14, 2024

Language: Английский

Citations

1
RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei DOI Creative Commons
Miriam Schalamun, Eva M. Molin, Monika Schmoll

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: July 3, 2023

Abstract Background Adaptation to complex, rapidly changing environments is crucial for evolutionary success of fungi. The heterotrimeric G-protein pathway belongs the most important signaling cascades applied this task. In Trichoderma reesei, enzyme production, growth and secondary metabolism are among physiological traits influenced by in a light dependent manner. Results Here, we investigated function SNX/H-type regulator (RGS) protein RGS4 T. reesei . We show that involved regulation cellulase growth, asexual development oxidative stress response darkness as well osmotic presence sodium chloride, particularly light. Transcriptome analysis revealed several ribosomal genes, six genes mutated RutC30 encoding transcription factors transporters. Importantly, positively regulates siderophore cluster responsible fusarinine C biosynthesis respective deletion mutant shows altered on nutrient sources related production such ornithine or proline BIOLOG phenotype microarray assay. Additionally, storage carbohydrates intermediates D-galactose D-arabinose catabolic decreased, predominantly Conclusions conclude mainly operates targets plant cell wall degradation, compound

Language: Английский

Citations

3
Trichoderma: Population Structure and Genetic Diversity of Species with High Potential for Biocontrol and Biofertilizer Applications DOI Creative Commons
Adnan Ismaiel, Dilip K. Lakshman, Prashant P. Jambhulkar

et al.

Applied Microbiology, Journal Year: 2024, Volume and Issue: 4(2), P. 875 - 893

Published: May 27, 2024

Certain Trichoderma isolates provide biofertilizer, biocontrol, and other plant-beneficial activities while inhabiting the soil or internal plant tissue, their use in agricultural systems can contribute to sustainable food production. It is thought that colonization of tissue fundamental for biocontrol biofertilizer applications. Our collective analyses prior surveys, where tef1α sequence was almost exclusively used identify species, showed from Harzianum complex clade, T. asperellum/T. asperelloides group, virens, hamatum, atroviride were prevalent and/or as endophytes. Population structure genetic diversity based on markers tef1α, rpb2, ITS investigated, new lineages with statistical bootstrap support within atroviride, asperellum, virens populations found. The nearest relatives some these species also revealed. Choosing among more than 500 known non-targeted evaluation screens applications time-consuming expensive. Preferentially selecting asperelloides, harzianum possibly may speed identification candidates commercialization due demonstrated ability successfully inhabit tissue. To our knowledge, this first report dominant endophytic identified past survey data population conducted.

Language: Английский

Citations

0
Genomic Characterization and Establishment of a Genetic Manipulation System for Trichoderma sp. (Harzianum Clade) LZ117 DOI Creative Commons
Jie Yang,

Cristopher Reyes Loaiciga,

Hou-Ru Yue

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(10), P. 697 - 697

Published: Oct. 7, 2024

species have been reported as masters in producing cellulolytic enzymes for the biodegradation of lignocellulolytic biomass and biocontrol agents against plant pathogens pests. In our previous study, a novel

Language: Английский

Citations

0
Assembly, Annotation, and Comparative Analysis of Mitochondrial Genomes in Trichoderma DOI Open Access

Xiaoting Wang,

Zhiyin Wang,

Fanxing Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12140 - 12140

Published: Nov. 12, 2024

Trichoderma is a widely studied ascomycete fungal genus, including more than 400 species. However, genetic information on limited, with most species reporting only DNA barcodes. Mitochondria possess their own distinct that plays pivotal role in molecular function and evolution. Here, we report 42 novel mitochondrial genomes (mitogenomes) combined 18 published mitogenomes of Trichoderma. These circular exhibit sizes 26,276–94,608 bp, typically comprising 15 core protein-coding genes (PCGs), 2 rRNAs, 16–30 tRNAs; however, the number endonucleases hypothetical proteins encoded introns PCGs increases genome size enlargement. According to result phylogenetic analysis whole mitogenome, these strains diverged into six evolutionary branches, supported by phylogeny based 2830 single-copy nuclear genes. Comparative revealed dynamic exhibited variations size, gene number, GC content, tRNA copy, intron across different branches. We identified three mutation hotspots near regions encoding nad3, cox2, nad5 caused major changes mitogenomes. Evolutionary atp9, cob, nad4L, nad5, rps3 have been influenced positive selection during This study provides valuable resource for exploring important roles dynamics mitogenome adaptive evolution biocontrol fungi.

Language: Английский

Citations

0
Genomes and Genomics of the Genus Trichoderma DOI
Monika Schmoll

Published: Jan. 1, 2024

Language: Английский

Citations

0
RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei DOI Creative Commons
Miriam Schalamun,

Eva-Maria Molin,

Monika Schmoll

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 3, 2023

Abstract Adaptation to complex, rapidly changing environments is crucial for evolutionary success of fungi. The heterotrimeric G-protein pathway belongs the most important signaling cascades applied this task. In Trichoderma reesei , enzyme production, growth and secondary metabolism are among physiological traits influenced by in a light dependent manner. Here, we investigated function SNX/H-type regulator (RGS) protein RGS4. We show that RGS4 involved regulation cellulase growth, asexual development stress response. Transcriptome analysis revealed six genes mutated RutC30 as well several encoding transcription factors transporters. Importantly, positively regulates siderophore cluster responsible fusarinine C biosynthesis respective deletion mutant shows altered on nutrient sources related production. Additionally, storage carbohydrates intermediates D-galactose D-arabinose catabolic decreased, predominantly light. conclude mainly operates targets plant cell wall degradation, production compound T. .

Language: Английский

Citations

0
Plant recognition byTrichoderma harzianumelicits upregulation of a novel secondary metabolite cluster required for colonization DOI Creative Commons
Miriam Schalamun,

Guofen Li,

Wolfgang Hinterdobler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 12, 2023

Abstract Trichoderma harzianum is a filamentous ascomycete frequently applied as biocontrol agent in agriculture. While mycoparasitism and antagonism of spp. against fungal pathogens are well known, early responses to the presence plant await broader investigation. Analyzing stages plant-fungus communication we show that T. B97 chemotropically responds extract both fungus alter secondary metabolite secretion upon recognition. We developed strategy for omics-analysis simulating conditions recognition eliciting chemotropic response found 102 genes be differentially regulated, including nitrate nitrite reductases. Additionally, previously uncharacterized P lant C ommunication A ssociated (PCA) gene cluster was strongly induced plant, comprises palindromic DNA motif essential colonization. The PCA-cluster only present Harzianum clade closely related homologous Metarhizium Horizontal transfer (HGT) detected by plants, while likely under balancing or positive selection. Hence, mediates fungus-plant chemical may responsible high potential species applications. Plain language summary Interactions plants with fungi – beneficial pathogenic crucial ecological function partners. Yet, “language” they use how when it still insufficiently known. describe discovery novel cluster, which plant-fungal interkingdom interaction biocontrol-agent .

Language: Английский

Citations

0
Effects of Blue-Light Laser Irradiation on the Enzymatic Activities and Sporulation of Trichoderma atroviride Grown on Rice Husks DOI Creative Commons

Ioana-Alexandra Bala,

Naomi Tritean,

Alin Enache

et al.

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(16), P. 9191 - 9191

Published: Aug. 12, 2023

Light is known to impact various aspects of Trichoderma, with possible implications for industrial and agricultural applications. In this study, we investigated the irradiation Trichoderma atroviride blue light using a laser source system. We determined cellulase protease activities effects UV–Vis absorption filtrated culture on conidia formation, dimension, behavior, apparent chlamydospore abundance, as function duration, dose irradiation, moment irradiation. show that enzymatic range from positive neutral negative. Compared previous studies, our stimulation does not drastic negative effect (CMC-ase) activity, but it seems delay maximum activity over time. The proteases are partially in agreement only study reported activity. number increased upon representing an expected first time best knowledge dimensions also increase. Our data suggest highest resulted clustering around liquid–air interface, suggesting hydrophobic character.

Language: Английский

Citations

0