Phylogenetic and functional analyses ofN⁶-methyladenosine RNA methylation factors in the wheat scab fungusFusarium graminearum

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 12, 2023

Abstract In eukaryotes, N 6 -methyladenosine (m A) RNA modification plays crucial roles in governing the fate of molecules and has been linked to various developmental processes. However, phyletic distribution functions genetic factors responsible for m A remain largely unexplored fungi. To get insights into evolution machineries, we reconstructed global phylogenies potential writers, readers, erasers Substantial copy number variations were observed, ranging from up five writers early-diverging fungi a single subphylum Pezizomycotina, which primarily comprises filamentous characterize phytopathogenic fungus Fusarium graminearum , generated knockout mutants lacking including sole writer MTA1 . resulting knockouts did not exhibit any noticeable phenotypic changes during vegetative sexual growth stages. As obtaining homozygous was likely hindered by its essential role, -overexpressing strains ( -OE). The -OE5 strain showed delayed conidial germination reduced hyphal branching, suggesting involvement growth. Consistent with these findings, expression levels reader YTH1 dramatically induced germinating conidia, followed at later Several genes transcription factors, transporters enzymes found be significantly up- down-regulated strain. Overall, our study highlights functional importance methylation F. provides foundation future investigations sites Importance is reversible posttranscriptional that regulates function role diverse This addresses knowledge gap regarding identification among fungal groups enriches machinery Functional characterization branching. observed effects overexpressing on gene patterns throughout life cycle further underscore germination. this advances understanding fungi, paving way research applications disease control.

Language: Английский

Characterization of Fungal Microbiome Structure in Leaf Litter Compost through Metagenomic Profiling of the Nuclear Ribosomal Internal Transcribed Spacer Region

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 3, 2023

Abstract This study addressed the significant but understudied role of fungal communities in leaf litter and organic waste compost ecosystems, using advanced ITS metagenomic profiling. The research aims to comprehensively characterize these better understand their diversity, composition, potential functions, with practical implications for optimizing composting processes promoting sustainable management practices. Various samples were collected, including composts other matured composts, from different sources Delhi-National Capital Region, India. DNA was extracted amplified PCR obtain sequence internal transcribed spacer (ITS) region. It followed by sequencing bioinformatics analysis microbiome assess diversity within ecosystems. Results revealed that cow dung manure had highest read counts, while kitchen lowest. Alpha indices, Chao1 Shannon, exhibited differences species richness among though statistical significance limited. also identified dominant phyla such as Ascomycota, Basidiomycota, Mucoromycota , Zoopagomycota across composts. presence beneficial genera like Pichia, Trichoderma, Mortierella, Basidiobolus well pathogenic viz. Alternaria, Fusarium Acremonium underscored need proper practices source selection optimize soil fertility minimize disease risks agriculture. Overall, findings contribute processes, advancing practices, enhancing use a bio-organic fertilizer. results highlighted monitoring quality its impact on health agricultural

Language: Английский