Gene
editing
refers
to
the
deletion,
replacement,
and
insertion
of
target
genes
obtain
new
functions.
Nowadays,
ZFN,
TALEN
CRISPR-Cas
are
known
as
three
major
technologies
gene
which
used
widely
on
more
occasions.
The
common
points
these
tools
that
recognition
region
contains
DNA
sequence
cleavage
functional
region.
As
a
revolutionary
tool
in
field
life
sciences,
its
derivative
may
revolutionize
biological
research
affect
personalized
medicine
future.
Herein,
feature
system
process
discovery
were
summarized.
Additionally,
principle
mechanism
was
described
detail
this
paper.
Besides,
also
played
an
important
role
plant-related
fields
therapy
mentioned.
However,
there
problems
application
technology,
discussion
started
from
ethical
controversy
brought
about
by
technology.
This
review
aims
deepen
understanding
gain
deeper
functions,
provide
theoretical
support
for
subsequent
research.
Plants,
Journal Year:
2023,
Volume and Issue:
12(21), P. 3693 - 3693
Published: Oct. 26, 2023
Fruit
trees
and
other
fruiting
hardwood
perennials
are
economically
valuable,
there
is
interest
in
developing
improved
varieties.
Both
conventional
breeding
biotechnology
approaches
being
utilized
towards
the
goal
of
advanced
cultivars.
Increased
knowledge
effectiveness
efficiency
can
help
guide
use
CRISPR
gene-editing
technology.
Here,
we
examined
CRISPR-Cas9-directed
genome
editing
valuable
commodity
fruit
tree
Malus
x
domestica
(domestic
apple).
We
transformed
two
cultivars
with
dual
CRISPR-Cas9
constructs
designed
to
target
AGAMOUS-like
genes
simultaneously.
The
main
was
determine
this
approach
for
achieving
gene
changes.
obtained
6
Cas9
control
38
independent
events.
Of
events,
34
(89%)
had
edits
14
(37%)
showed
changes
all
alleles
both
genes.
most
common
change
large
deletions,
which
were
present
59%
changed
alleles,
followed
by
small
deletions
(21%),
insertions
(12%),
a
combination
(8%).
Overall,
high
rate
successful
alterations
found.
Many
these
predicted
cause
frameshifts
peptides.
Future
work
will
include
monitoring
floral
development
form.
Frontiers in Life Sciences and Related Technologies,
Journal Year:
2024,
Volume and Issue:
5(1), P. 31 - 37
Published: March 28, 2024
To
investigate
the
effects
of
heat
shock
proteins
(HSPs)
on
walnut
(Juglans
regia
L.)
plants
under
low-temperature
stress,
first
all,
tolerances
ten
cultivars
(Chandler,
Fernor,
Franquette,
Pedro,
Bilecik,
Kaman-I,
Kaman-II,
Kaman-III,
Sebin,
and
Yalova
I)
were
determined.
One-year-old
shoot
samples
taken
from
in
two
different
periods,
cold-acclimated
(CA)
non-acclimated
(NA),
exposed
to
+5°C,
-5°C,
-15°C
-25°C
for
12
h.
Cold
injury
was
determined
by
ion
leakage
analysis
thawed
bark
tissues.
According
results
this
analysis,
as
cold-sensitive
(Chandler)
cold-tolerant
(Fernor)
terms
tolerance.
examine
HSPs
cold
tolerance
plants,
protein
profiles,
amount
total
protein,
these
As
a
result
immunoblot
it
that
44
kDa
HSP23
59
HSP60
are
responsible
plants.
