<i>Agrobacterium</i>-Mediated Genome Modification for Improvement of Oil Palm Planting Materials

OBM Genetics, Journal Year: 2025, Volume and Issue: 09(02), P. 1 - 28

Published: April 15, 2025

Oil palm is the most productive vegetable oil crop compared to other oil-bearing crops because it produces highest yield per hectare. Palm very versatile since used for producing food and beverages, personal care cosmetics, cleaning products, biofuel, bioenergy. To cater increasing demand in global market, much research has been done improve oil's modify its quality addition height through breeding. Due long breeding cycle, planting materials have improved using biotechnological approaches such as genetic engineering genome editing. The ability transform with high efficiency key effect modification of palm. current transformation <em>Agrobacterium</em>-mediated low monocots rice, maize, wheat. Over last few decades, numerous studies conducted enhance efficiency, providing a more reliable landscape CRISPR/Cas9 In this review, we discussed progress made transformation, focusing on outline possible strategies regeneration efficiencies. prospect editing improving palms agronomic traits, yield, plant height, fruit color, resistance resist biotic abiotic stresses, were also discussed.

Language: Английский

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Engineering tomato disease resistance by manipulating susceptibility genes

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: Feb. 10, 2025

Various pathogens severely threaten tomato yield and quality. Advances in understanding plant-pathogen interactions have revealed the intricate roles of resistance (R) susceptibility (S) genes determining plant immunity. While R provide targeted pathogen resistance, they are often vulnerable to evolution. Conversely, S offer a promising avenue for developing broad-spectrum durable through gene editing. Recent breakthroughs CRISPR/Cas-based technologies revolutionized manipulation genomes, enabling precise modification enhance disease without compromising growth or However, utilization full potential this technique is challenging due complex current technological limitations. This review highlights key advances using editing tools dissect engineer improved We discuss how influence entry, immune suppression, nutrient acquisition, their has conferred bacterial, fungal, viral pathogens. Furthermore, we address challenges associated with growth-defense trade-offs propose strategies, such as hormonal pathway modulation regulatory edits, overcome these underscores CRISPR-based approaches transform breeding, paving way sustainable production disease-resistant cultivars amidst escalating global food security challenges.

Language: Английский

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Emerging Pests and Disease Vectors

Published: Jan. 1, 2025

Language: Английский

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Bioinformatics-Based Management of Vitellogenin-like Protein’s Role in Pathogen Defense in Nicotiana tabacum L.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(8), P. 4463 - 4463

Published: April 18, 2025

The primary objective of this study was to identify and characterize pathogen defense proteins in the Nicotiana tabacum L. proteome, focusing on their structural, functional, evolutionary properties, as well interactions with pathogen-derived molecules. Specifically, we aimed comprehensively analyze proteome pinpoint potential uncharacterized defense-related protein that has emerging roles immune responses antioxidant activity across plants animals. Through integrated computational approaches, determined relationships, structural modeling selected performed using different software, followed by validation through multiple metrics, including stereochemical checks (Ramachandran plot), MolProbity analysis, Z-scores. We further investigated functional binding regions or interaction sites. molecular docking investigate between pathogen-associated patterns (PAMPs), specifically β-glucan peptidoglycan (PGN), elucidate defensive mechanisms. Last, normal mode analysis (NMA), dynamics simulation (MDS), post-simulation analyses were employed evaluate stability mobility protein–ligand complexes. Uncharacterized vitellogenin-like (VLP: ID A0A1S4CXB2) domain chosen because its predicted immune-related features, stress response patterns, unknown role at new immunity functions. Phylogenetic revealed significant sequence homology VLPs from other members Solanaceae family. Structural showed a high-quality model, studies indicating stronger affinity for PGN (−10.16 kcal/mol) (−7.19 kcal/mol), highlighting involvement defense. NMA, MDS, exhibits more substantial extensive VLP than β-glucan. Our findings confirmed N. may function pattern recognition receptors (PRRs), capable recognizing responding pathogens activating signaling pathways. Future experimental these could plant application biotechnological approaches sustainable agriculture.

Language: Английский

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Genetic Insights and Molecular Breeding Approaches for Downy Mildew Resistance in Cucumber (Cucumis sativus L.): Current Progress and Future Prospects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12726 - 12726

Published: Nov. 27, 2024

Cucurbit downy mildew, caused by Pseudoperonospora cubensis, is a devastating disease in cucumbers that leads to significant yield losses many cucurbit-growing regions worldwide. Developing resistant cucumber varieties sustainable approach managing this disease, especially given the limitations of chemical control and evolving nature pathogens. This article reviews genetic basis mildew resistance cucumbers, emphasizing key (R) genes quantitative trait loci (QTLs) have been mapped. Recent advances molecular breeding tools, including marker-assisted selection (MAS), genomic (GS), CRISPR/Cas9 genome editing, accelerated development cultivars. review also explores role transcriptomics, genomics, other ‘omics’ technologies unraveling mechanisms behind offers insights into future strategies aimed at long-term management. Management for cucurbit are discussed, along with potential impacts climate change on occurrence severity highlighting how changing environmental conditions may influence dynamics. Integrating these advanced approaches traditional promises accelerate mildew-resistant varieties, contributing sustainability resilience production.

Language: Английский

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Genetic Improvements in Aquaculture

Published: Nov. 28, 2024

In the shift from wild harvesting to agricultural production, genetic enhancement plays a crucial role in development of product. The initial phase typically involves domestication, followed by further modifications aimed at improving production traits. Aquaculturists, like their terrestrial counterparts, prioritize traits such as rapid growth, increased efficiency, disease resistance, and high quality. Various tools are utilized achieve these enhancements. This article seeks assess present state obstacles concerning administration farmed aquatic resources while offering suggestions for enhancement.

Language: Английский

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Ecotoxicity and Removal of Pesticides by Aquatic Plants

˜The œhandbook of environmental chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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