Guidelines for minimal information on cellular senescence experimentation in vivo

Cell, Journal Year: 2024, Volume and Issue: 187(16), P. 4150 - 4175

Published: Aug. 1, 2024

Cellular senescence is a cell fate triggered in response to stress and characterized by stable cell-cycle arrest hypersecretory state. It has diverse biological roles, ranging from tissue repair chronic disease. The development of new tools study vivo paved the way for uncovering its physiological pathological roles testing senescent cells as therapeutic target. However, lack specific broadly applicable markers makes it difficult identify characterize tissues living organisms. To address this, we provide practical guidelines called "minimum information cellular experimentation vivo" (MICSE). presents an overview rodent tissues, transgenic models, non-mammalian systems, human tumors their use identification specification cells. These uniform, state-of-the-art, accessible toolset improve our understanding vivo.

Language: Английский

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TRDMT1 exhibited protective effects against LPS‐induced inflammation in rats through TLR4‐NF‐κB/MAPK‐TNF‐α pathway

Animal Models and Experimental Medicine, Journal Year: 2022, Volume and Issue: 5(2), P. 172 - 182

Published: April 1, 2022

Abstract Background Inflammation is a complex physiological and pathological process. Although many types of inflammation are well characterized, their functions largely unknown. tRNA aspartic acid methyltransferase 1 (TRDMT1) has been implicated as stress‐related protein, but its intrinsic biological role unclear. Methods We constructed Trdmt1 knockout rat adopted the LPS‐induced sepsis model. Survival curve, histopathological examination, expression inflammatory factors, protein level TLR4 pathway were analyzed. Results deletion had no obvious impact on development growth. slightly increased mortality during aging. Our data showed that strongly responded in LPS‐treated rats, rats vulnerable to LPS treatment with declined survival rate. also observed more aggravated tissue damage cumulative functional cell degeneration compared control rats. Further studies upregulated TNF‐α liver, spleen, lung, serum tissues, which may be explained by enhanced p65 p38 phosphorylation. Conclusions demonstrated plays protective regulating TLR4‐NF‐κB/MAPK‐TNF‐α pathway. This work provides useful information understand TRDMT1 function inflammation.

Language: Английский

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Conservation at the uterine–placental interface

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(41)

Published: Oct. 3, 2022

The hemochorial placentation site is characterized by a dynamic interplay between trophoblast cells and maternal cells. These cooperate to establish an interface required for nutrient delivery promote fetal growth. In the human, penetrate deep into uterus. This not consistent feature of has hindered establishment suitable animal models. rat represents intriguing model investigating with cell invasion. this study, we used single-cell RNA sequencing characterize transcriptome invasive lineage, as well other populations within uterine–placental during early (gestation day [gd] 15.5) late (gd 19.5) stages intrauterine We identified robust set transcripts that define cells, distinguished endothelial, smooth muscle, natural killer, macrophage Invasive trophoblast, immune, endothelial exhibited distinct spatial relationships interface. Furthermore, maturation stage development could be determined assessing gestation stage–dependent changes in transcript expression. Finally, most importantly, expression prominent subset conserved extravillous lineage human placenta. findings provide foundational data identify interrogate key regulatory mechanisms essential function important compartment healthy pregnancy.

Language: Английский

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The complex affective and cognitive capacities of rats

Science, Journal Year: 2024, Volume and Issue: 385(6715), P. 1298 - 1305

Published: Sept. 19, 2024

For several decades, although studies of rat physiology and behavior have abounded, research on emotions has been limited in scope to fear, anxiety, pain. Converging evidence for the capacity many species share others’ affective states emerged, sparking interest empathic capacities rats. Recent demonstrated that rats are a highly cooperative motivated by distress prosocial actions, such as opening door or pulling chain release trapped conspecifics. Studies affect, cognition, neural function provide compelling some represent needs, instrumentally act improve their well-being, thus capable forms targeted helping. Rats’ complex abilities raise importance integrating new measures well-being into scientific research.

Language: Английский

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Review of the Use of Animal Models of Human Polycystic Kidney Disease for the Evaluation of Experimental Therapeutic Modalities

Journal of Clinical Medicine, Journal Year: 2023, Volume and Issue: 12(2), P. 668 - 668

Published: Jan. 14, 2023

Autosomal dominant polycystic kidney disease, autosomal recessive and nephronophthisis are hereditary disorders with the occurrence of numerous cysts in both kidneys, often causing chronic end-stage renal failure. Animal models have played an important role recent advances research not only on disease onset progressive mechanisms but also development therapeutic interventions. For a long time, spontaneous animal been used as primary focus for human diseases; however, after identification nucleotide sequence responsible genes, PKD1, PKD2, PKHD1, NPHPs, various types genetically modified were developed by genetic reproductive engineering techniques leading field. In this review, we present murine cystic diseases, discussing their potential benefits strategies.

Language: Английский

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Rat Models of Breast Cancer

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 123 - 148

Published: Jan. 1, 2025

Language: Английский

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Establishment and characterization of liver‐specific Apoa4‐Cre and Cyp2c11‐Cre rat models in juvenile and adult stages

Animal Models and Experimental Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Background Liver diseases are a major contributor to both morbidity and mortality. Conditional knockout animals always produced through crossing floxed with tissue‐specific Cre animal. The use of rat resource has rapidly increased, but the liver‐specific lines for studying liver interested genes limited, especially in spatially temporally restricted manner. Methods RNA sequencing real‐time polymerase chain reaction (PCR) were used screen confirm presence genes. Apoa4 ‐Cre rats Cyp2c11 by CRISPR/Cas9 knockin. Rosa26‐imCherry employed hybridize obtain /Rosa26‐imCherry rats. temporal spatial patterns expression determined observation red fluorescence on tissue sections. Hematoxylin–eosin stain was evaluate histopathologic changes. blood biochemical analysis several enzymes lipid profile performed function Results identified as two hybridized indicated that recombinases specially expressed juvenile adult not other organs All parameters except low‐density lipoprotein (LDL) did change significantly No histological alterations detected livers Conclusions Liver‐specific have been established successfully could be study gene knockout, specifically liver.

Language: Английский

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Animal-based approaches to understanding neuroglia physiology in vitro and in vivo

Handbook of clinical neurology, Journal Year: 2025, Volume and Issue: unknown, P. 229 - 263

Published: Jan. 1, 2025

Language: Английский

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Can Humanized Immune System Mouse and Rat Models Accelerate the Development of Cytomegalovirus-Based Vaccines Against Infectious Diseases and Cancers?

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3082 - 3082

Published: March 27, 2025

Over the past three decades, immunodeficient mouse models carrying human immune cells, with or without lymphoid tissues, termed humanized system (HIS) rodent models, have been developed to recapitulate and associated responses. HIS successfully modeled many human-restricted viral infections, including those caused by cytomegalovirus (HCMV) immunodeficiency virus (HIV). also used model cancer immunobiology, which exhibits differences from murine cancers in traditional models. Variants of that carry liver lung tissue, skin patient-derived tumor xenografts hematopoietic stem cells-derived-human cells tissue probe responses infections tumors. HCMV-based vaccines are human-restricted, poses limitations for mechanistic efficacy studies using animal The vaccine approach is a promising strategy as it induces robust effector memory T cell may be critical preventing rapidly controlling persistent cancers. Here, we review novel development primary secondary tissues could address mice their use research. We reviewed rat allow long-term (greater than one year) vaccinology better pathophysiology. Translating laboratory research findings clinical application significant bottleneck development; rodents related variants more accurately immunology diseases increase translatability findings.

Language: Английский

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Neural correlates of visual object recognition in rats

Cell Reports, Journal Year: 2025, Volume and Issue: 44(4), P. 115461 - 115461

Published: March 29, 2025

Language: Английский

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