Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis

Cell Biology International, Journal Year: 2024, Volume and Issue: 48(8), P. 1097 - 1110

Published: May 5, 2024

Abstract Cisplatin is commonly used for the chemotherapy of tongue squamous cell carcinoma (TSCC); however, adverse side effects and drug resistance impact its therapeutic efficacy. Capsaicin an active ingredient in chili peppers that exerts antitumor effects, whether it on cisplatin‐resistant cells remains unknown. Therefore, this study, we investigated effect capsaicin cisplatin TSCC explored underlying mechanisms. A line was established by treated with increasing concentrations. Combined treatment decreased glucose consumption lactate dehydrogenase activity increased adenosine triphosphate production both vitro vivo, suggesting inhibition Warburg effect. Moreover, combined induced apoptosis significantly upregulated levels proapoptotic proteins, such as Bax, cleaved caspase‐3, ‐7, ‐9, apoptosis‐inducing factor. In contrast, antiapoptotic protein, Bcl‐2, were downregulated. Additionally, LKB1 AMPK activities stimulated, whereas those AKT mTOR suppressed. Notably, knockdown abolished inhibitory AKT/mTOR signaling pathway Overall, reversed inhibiting facilitating mitochondrial‐dependent via AMPK/AKT/mTOR axis. Our findings suggest combination therapy a potentially novel strategy highlight promising adjuvant treatment.

Language: Английский

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Role of epigenetic molecular mechanisms in insect evolutionary innovations

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 217 - 282

Published: Jan. 1, 2024

Language: Английский

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Unveiling Nilaparvata lugens Stål Genes Defining Compatible and Incompatible Interactions with Rice through Transcriptome Analysis and Gene Silencing

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(8), P. 6790 - 6803

Published: Aug. 16, 2023

The brown planthopper (Nilaparvata lugens Stål, BPH) is a major pest of rice (Oryza sativa L.), causing severe crop loss. Multiple biotypes and emerging populations BPH pose bigger challenge for the infestations control. Although several studies have been conducted to understand molecular mechanisms rice–BPH interactions, there are few dedicated Indian sub-continent biotype (biotype 4). Here, we analyzed transcriptomic, physiological, gene-silencing responses 4 during compatible (fed on susceptible Taichung Native 1, TN1 rice) incompatible resistant PTB33 interactions. In interaction, significant reduction in honeydew production negative weight gain were observed BPH. Similarly, trehalose glucose contents found be significantly high low, respectively, interaction. comparative transcriptome analysis identified 1875 differentially expressive genes (DEGs) between interactions from which many annotated involved vital physiological processes, including cuticle development, sugar metabolism, detoxification, molting, xenobiotics metabolism. RNA interference-mediated independent silencing three selected genes, NlCP1, NlCYP320a1, NlTret1, revealed that these important physiology survival. Moreover, results this study provide valuable insights into involving 4.

Language: Английский

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Differential Antioxidant Enzyme Gene Expression and Functional Analysis of Pyridaben-Susceptible and -Resistant Strains of Tetranychus truncatus (Acari: Tetranychidae) under High Temperature Stress

Insects, Journal Year: 2024, Volume and Issue: 15(6), P. 381 - 381

Published: May 23, 2024

Tetranychus truncatus (Acari: Tetranychidae) has caused serious economic losses on some crops (soybean, corn, and cotton) in China, developed resistance to most acaricides. Our laboratory study found that T. was resistant pyridaben also adapted high temperature (34–40 °C). High stress may cause arthropods produce a large amount of reactive oxygen species (ROS), causing oxidative damage. Antioxidant enzymes, as the main antioxidants, can reduce damage by excessive ROS arthropods. In order adaptation mechanism pyridaben-resistant strain role antioxidant enzyme genes under stress, four genes, TtSOD, TtPOD3, TtPOD4, TtGSTs2, were screened according transcriptome sequencing data pyridaben-susceptible -resistant strains truncatus. Firstly, phylogeny structure analyses these carried out. Then, real-time quantitative PCR (RT-qPCR) technology used analyze gene expression patterns enzymes two at three different ranges (34 °C, 38 42 The results showed levels induced significantly each development state. adult stage higher than susceptible strains. After TtSOD TtPOD4 mites silenced RNA interference (RNAi) technology, mortality rate with silencing reached 41.11% after 96 h 34 which control silencing. It been confirmed plays key temperature. lays theoretical foundation for revealing thermal

Language: Английский

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Insect adaptation: unveiling the physiology of digestion in challenging environments

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 2, 2024

Insect's resilience to adverse conditions poses a significant challenge for integrated pest control. This has resulted in huge economic losses agriculture and forestry production as well range of severe ecological issues. As physiological mechanism insects, digestive physiology plays an important role the process adaptation stress factors. However, there been no systematic review what stresses insects can adapt through how is involved insect stresses. In this review, potential link between biotic abiotic stresses, including plant defense mechanisms, chemical insecticides, entomopathogenic microorganisms, analyzed. We point that composed enzymes gut microbial communities strategy resist physical (e.g., hemicellulose, pectin, microfibers), azadirachtin, diterpenoid acids, phenolic glycosides), insecticide stress, microorganism infection. addition, primary function ensure energy supply during assist metabolism exogenous toxins anti-insect proteins, metabolites, secondary insecticides), improve their innate immunity against microorganisms. helpful elucidate adversity, provide breakthrough analyzing causes outbreaks.

Language: Английский

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Effects of Global Warming on Insects: Current Approaches

Published: May 31, 2024

This chapter discusses the extent in which insects are affected by global warming, but since it is hardly possible to fully disclose and examine effects of warming on insects, most populous group world, just one or even a book, we have assumed here task provide general overview relation some current studies approaches context climate change. We consider level these an order basis, what concerns objects study covered especially responses (particularly behavioural-migration one). Finally, also envisage scenarios that may occur under warming.

Language: Английский

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Epigenomics: A Way Forward from Classical Approach

Published: Jan. 1, 2024

Language: Английский

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Histone deacetylases synergistically regulate juvenile hormone signaling in the yellow fever mosquito, Aedes aegypti

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: unknown, P. 104256 - 104256

Published: Dec. 1, 2024

Language: Английский

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A Review of Acaricides and Their Resistance Mechanisms in Hard Ticks and Control Alternatives with Synergistic Agents

Acta Tropica, Journal Year: 2024, Volume and Issue: unknown, P. 107519 - 107519

Published: Dec. 1, 2024

Language: Английский

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