The Overexpression of the Bptcp20 Gene Enhances Cell Proliferation and Improves Tolerance to Drought and Salt Stress in Betula Platyphylla DOI

Huiyu Li,

Yue Li, Yan Shi

et al.

Published: Jan. 1, 2023

TCP transcription factors constitute a class of plant-specific transcriptional regulators that govern plant morphology and structure, contributing to the evolution diversity. They are also implicated in various vital biological processes, including gametophyte development, circadian rhythms, hormone signaling. However, precise underlying physiological mechanisms remain largely unexplored. In this study, we discovered factor family proteins exhibit remarkable conservation, with an impressive 98% homology observed between BpTCP20 AtTCP20. The gene plays crucial role leaf as well mediating responses hormonal abiotic stress factors. Notably, Betula platyphylla plants overexpressing displayed increased seedling height, ground diameter, area compared their wild-type counterparts, leading significant enhancement drought salt tolerance. Further investigations demonstrated that, under conditions stress, exhibited lower indices, hydrogen peroxide levels, malondialdehyde contents plants. Conversely, chlorophyll peroxidase significantly, along upregulation superoxide dismutase related genes. These findings strongly indicate positive regulatory enhancing tolerance platyphylla. Utilizing RNA sequencing, identified pathways associated growth Furthermore, through Yeast one-hybrid assays, uncovered interactions BpMYB8 BpIAA5. This study sheds light on function regulation development strains, response stress. It underscores potential candidate for

Language: Английский

Physiological Roles and Mechanisms of Action of Class I TCP Transcription Factors DOI Open Access
Ivana L. Viola, Antonela L. Alem,

Rocío M. Jure

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5437 - 5437

Published: March 12, 2023

TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTOR 1 and 2 (TCP) proteins constitute a plant-specific transcription factors family exerting effects on multiple aspects of plant development, such as germination, embryogenesis, leaf flower morphogenesis, pollen through the recruitment other modulation different hormonal pathways. They are divided into two main classes, I II. This review focuses function regulation class TCP (TCPs). We describe role TCPs in cell growth proliferation summarize recent progresses understanding diverse developmental processes, defense, abiotic stress responses. In addition, their redox signaling interplay between involved immunity transcriptional posttranslational is discussed.

Language: Английский

Citations

27
TCP Transcription Factors in Plant Reproductive Development: Juggling Multiple Roles DOI Creative Commons
Ivana L. Viola, Daniel H. González

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 750 - 750

Published: April 26, 2023

TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors (TFs) are plant-specific transcriptional regulators exerting multiple functions in plant growth and development. Ever since one of the founding members family was described, encoded by CYCLOIDEA (CYC) gene from Antirrhinum majus involved regulation floral symmetry, role these TFs reproductive development established. Subsequent studies indicated that CYC clade TCP were important for evolutionary diversification flower form a multitude species. In addition, more detailed function TCPs other clades revealed roles different processes related to development, such as flowering time, inflorescence stem, correct organs. this review, we summarize during well molecular networks their action.

Language: Английский

Citations

23
Flowering time genes branching out DOI Creative Commons
Pierangela E Colleoni, Sam W. van Es, Ton Winkelmolen

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(14), P. 4195 - 4209

Published: March 11, 2024

Abstract Plants are sessile by nature, and as such they have evolved to sense changes in seasonality their surrounding environment, adapt these changes. One prime example of this is the regulation flowering time angiosperms, which precisely timed coordinated action two proteins: FLOWERING LOCUS T (FT) TERMINAL FLOWER 1 (TFL1). Both regulators members PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN (PEBP) family proteins. These regulatory proteins do not interact with DNA themselves, but instead transcriptional regulators, D (FD). FT TFL1 were initially identified key time, acting through binding FD; however, PEBP also involved shaping plant architecture development. In addition, PEBPs can TCP TEOSINTE BRANCHED (TB1), a well-known regulator architecture, domestication-related genes many crops. Here, we review role As yield-related traits, highlight examples from model Arabidopsis well important food feed crops as, rice, barley, wheat, tomato, potato.

Language: Английский

Citations

9
Genome-wide characterization of TCP family and their potential roles in abiotic stress resistance of oat (Avena sativa L.) DOI Creative Commons

Jing Pan,

Zeliang Ju, Xiang Ma

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 8, 2024

The TCP gene family members play multiple functions in plant growth and development were named after the first three found this family, TB1 (TEOSINTE BRANCHED 1), CYCLOIDEA (CYC), Proliferating Cell Factor 1/2 (PCF1/2). Nitrogen (N) is a crucial element for forage yield; however, over-application of N fertilizer can increase agricultural production costs environmental stress. Therefore, discovery low tolerance genes essential genetic improvement superior oat germplasm ecological protection. Oat ( Avena sativa L.), one world’s staple grass forages, but no genome-wide analysis their roles low-nitrogen stress has been performed. This study identified using bioinformatics techniques. It analyzed phylogeny, structure analysis, expression patterns. results showed that AsTCP includes 49 members, most AsTCP-encoded proteins are neutral or acidic proteins; phylogenetic tree classified into subfamilies, each subfamily different conserved structural domains functions. In addition, cis-acting elements detected promoter genes, which associated with abiotic stress, light response, hormone response. from unevenly distributed on 18 chromosomes. real-time quantitative polymerase chain reaction (qRT-PCR) had levels various tissues under nitrogen indicated these (such as AsTCP01 , AsTCP03 AsTCP22 AsTCP38 ) played oat. conclusion, potential at level, lays foundation further provides theoretical basis exploration excellent an future in-depth studies other genera reveals new research ideas to improve utilization.

Language: Английский

Citations

6
Plant gene networks involved in drought stress response and tolerance DOI
Sourobh Maji, Nidhi Dwivedi

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 403 - 426

Published: Jan. 1, 2025

Language: Английский

Citations

0
TCP23-WRKY15 module negatively regulates lignin deposition and xylem development of wood formation in Populus DOI
Shengkui Zhang, Can Li, Baihui Cui

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141656 - 141656

Published: March 1, 2025

Language: Английский

Citations

0
Systematic Analysis of the Betula platyphylla TCP Gene Family and Its Expression Profile Identifies Potential Key Candidate Genes Involved in Abiotic Stress Responses DOI Creative Commons
Shengzhou Guo,

Yuan Xu,

Yi Zhou

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 880 - 880

Published: March 11, 2025

The TCP transcription factor (TF) family is a vital set of plant-specific regulators involved in plant growth, development, and responses to environmental stresses. Despite the extensive research on factors numerous species, functions they fulfill Betula platyphylla are still not well understood. In this study, 21 BpTCP genes were identified via genome-wide analysis. Bioinformatics analysis was used examine physicochemical properties these factors, including molecular weight, isoelectric point, chromosomal distribution, predicted subcellular localization. We expected that most would be located nucleus. Collinearity revealed gene fragment duplication events played major role evolutionary expansion diversification family. Promoter diverse cis-acting elements BpTCP, suggesting play stress responses, hormonal regulation, growth development. qRT-PCR showed displayed tissue-specific expression patterns roots, stems, leaves, displaying remarkable differences levels when subjected abiotic stresses, drought high- low-temperature conditions. Notably, BpTCP17 BpTCP18 markedly higher under multiple Subcellular localization experiments confirmed both localize nucleus, consistent with bioinformatic predictions. These findings emphasize potential roles mediating highlighting their as candidate for improving tolerance B. platyphylla.

Language: Английский

Citations

0
Genome-wide identification and expression analysis of the TCP transcription factor family and its response to abiotic stress in rapeseed (Brassica napus L.) DOI Creative Commons

Xinrui Xu,

Xin Zhang, Yu Fan

et al.

3 Biotech, Journal Year: 2025, Volume and Issue: 15(5)

Published: April 7, 2025

Language: Английский

Citations

0
Systematic Characterization of GATA Transcription Factors in Liriodendron chinense and Functional Validation in Abiotic Stresses DOI Creative Commons
Delight Hwarari,

Yasmina Radani,

Yuanlin Guan

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(12), P. 2349 - 2349

Published: June 16, 2023

The

Language: Английский

Citations

9
Genome-wide identification and expression analysis of TCP family genes in Catharanthus roseus DOI Creative Commons
Juan Hao, Lijun Zheng,

Yidie Han

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 12, 2023

The anti-tumor vindoline and catharanthine alkaloids are naturally existed in Catharanthus roseus (C. roseus), an ornamental plant many tropical countries. Plant-specific TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play important roles various developmental processes. However, the of C. TCPs (CrTCPs) terpenoid indole alkaloid (TIA) biosynthesis largely unknown. Here, a total 15 CrTCP genes were identified newly updated genome grouped into three major classes (P-type, C-type CYC/TB1). Gene structure protein motif analyses showed that CrTCPs have diverse intron-exon patterns distributions. A number stress responsive cis-elements promoter regions CrTCPs. Expression analysis (CrTCP2, CrTCP4, CrTCP7) expressed specifically leaves four (CrTCP13, CrTCP8, CrTCP6, CrTCP10) flowers. HPLC contents classic TIAs, vindoline, ajmalicine, significantly increased by ultraviolet-B (UV-B) methyl jasmonate (MeJA) leaves. By analyzing expression under UV-B radiation MeJA application with qRT-PCR, TIA biosynthesis-related to be treatments. Interestingly, two TCP binding elements (GGNCCCAC GTGGNCCC) several genes, suggesting they potential target These results suggest involved regulation provide basis for further functional identification

Language: Английский

Citations

8