DEVELOPMENT OF METHODS OF BREEDING WORK WITH INSECTS OF THE SPECIES HERMETIA ILLUCENS

Siberian Journal of Life Sciences and Agriculture, Journal Year: 2023, Volume and Issue: 15(2), P. 74 - 91

Published: April 30, 2023

Background. The insect Hermetia illucens (black soldier fly), is widely used for the disposal of food and agricultural waste. This due to omnivorous nature fly in relation protein carbohydrate foods a fast reproductive cycle. big problem industrial cultivation this degeneration populations black fly. When bred captivity, relatively small number population, degradation key parameters, such as viability, fertility, productivity, etc. H. culture occurs. It noted that during first five generations, despite polygamy crossing, effect harmful recessive alleles homozygous state manifests itself. Currently, flies associated with inbred depression solved by mass large outbred cages, periodic reintroduction producers from natural populations. Purpose. Development method crossing lines breeding, determining patterns population development, well increasing their productivity adaptive potential. Materials Methods. In selection work species illucens, individual was used. Individual selections were made selecting larvae pupae size body weight. crosses carried out according scheme: one male female placed plastic containers while maintaining required temperature humidity. Eggs obtained pair on nutrient substrate separate container, hatched cultured isolation same container. During growth process, largest selected. After formation adult individuals (images) flies, they examined, sex determined again Thus, isofemale (descendants female) obtained. Results. We developed it possible trace development male, consider changes population. tendency determined, each subsequent generation there fewer fertilized eggs larvae. fact lethal genes become manifest effect. Subsequent most active will create devoid genes. Conclusions. As result research out, develop males females illucens. time oviposition onset maximum occurs 3-4th day after egg laying. With close relatives, manifestation lethals. genes. Populations have stable characteristics and, decrease clutches, smaller them, normal distribution weight, which allows economically valuable traits.

Language: Английский

Unravelling the genetics of fat accumulation in the house fly to foster circular economy

Published: Dec. 8, 2023

The world population is growing at an unprecedent rate and solutions to sustainably feed the increasing are urgently needed. Insects rich in protein lipids hence they represent a valid alternative source of these nutrients. Selecting for leaner or fatter phenotype insects could improve their application as food allow convert organic material into resources destined other applications respectively. To become successful target selection, trait must meet specific criteria such being genetically determined, heritable, showing potential respond selection. In this thesis I aim explore genetics fat accumulation house fly answer if meets three criteria. Chapter 2, provide general overview most up-to-date information on biology with particular emphasis its nutritional ecology, industrial species, relevant aspects, particularly light insect level. 2 wants be compendium those interested intriguing organism guide that want harness purposes. 3 first important question understand artificial selection: it determined? Chapter, used strains originated from different European locations. These were tested common environment two temperatures investigate ontogeny reserves. results experiment show there strong effect strain reserves, suggesting genetic component determines trait. 4 answering second about fly: heritable trait? Here estimated narrow sense heritability one by using half-sib paternal experimental design statistical models. High estimates found models giving similar results. discuss meaning our result selecting flies improved characteristics. 5 last regarding answered: can selection? evolution test response selective pressure. exposed larvae multiple generations nutritionally challenging diets. work we diets induced direct, plastic responses storage. Moreover, also when reversed ancestral diet, lines administrated differed significantly providing proof long lasting changes have occurred strains. Lastly, dedicate 6 discussion obtained give synthesis possibility select fat-tailored phenotypes content included goal selection program.

Language: Английский