Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants DOI Creative Commons
Pengcheng Wang, Ping Hu, Jinping Zhang

et al.

Avian Research, Journal Year: 2023, Volume and Issue: 14, P. 100078 - 100078

Published: Jan. 1, 2023

Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, has been mostly evaluated through techniques that fail to estimate diversity at whole genomic level. Next generation sequencing can meet this demand, but high quality samples such as blood or muscle tissues are required. it difficult collect from because sampling work may impact their health. Therefore, design a workflow evaluate using non-destructive sampling. Even though used traditional barcoding technique, technique cannot status. Brown Eared Pheasant (Crossoptilon mantchuricum) endangered species, with captive populations maintained Taiyuan Zoo, China, and Europe. diversity, inbreeding pattern, mutation load these two unclear. To uncover populations, we applied 2b-RAD technology feathers samples. The could be collected by results indicate Zoo population lower higher coefficient than European population. lethal mutations when homozygous. current project uses providing paradigm for genetics, which will facilitate development biology.

Language: Английский

Signs of local adaptation by genetic selection and isolation promoted by extreme temperature and salinity in the Mediterranean seagrass Posidonia oceanica DOI
Hung Manh Nguyen, Miriam Ruocco, Emanuela Dattolo

et al.

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(15), P. 4313 - 4328

Published: June 4, 2023

Adaptation to local conditions is known occur in seagrasses; however, knowledge of the genetic basis underlying this phenomenon remains scarce. Here, we analysed Posidonia oceanica from six sites within and around Stagnone di Marsala, a semi-enclosed coastal lagoon where salinity temperature exceed generally described tolerance thresholds species. Sea surface temperatures (SSTs) were measured plant samples collected for assessment morphology, flowering rate screening genome-wide polymorphisms using double digest restriction-site-associated DNA sequencing. Results demonstrated more extreme SSTs levels inside than outer regions. Morphological results showed significantly fewer shorter leaves reduced rhizome growth P. inner past events recorded only meadow farthest away lagoon. Using an array 51,329 single nucleotide polymorphisms, revealed clear structure among study confirmed isolation high clonality innermost site. In all, 14 outlier loci identified annotated with several proteins including those relate stress response, protein transport regulators plant-specific developmental events. Especially, five maximum allele frequency at site, likely reflecting adaptation regimes, possibly due selection resistant genotypes progressive restriction gene flow. Overall, helps us disentangle seagrass environmental may support future works on assisted evolution seagrasses.

Language: Английский

Citations

11
Genetic structure and diversity of the seagrass Zostera marina along a steep environmental gradient, with implications for genetic monitoring DOI Creative Commons
S. Ries, E. C. Faust, Kerstin Johannesson

et al.

Frontiers in Climate, Journal Year: 2023, Volume and Issue: 5

Published: Dec. 18, 2023

Zostera marina (eelgrass) is a foundation species in coastal zones the northern hemisphere. Eelgrass declining across its distribution, trend likely to accelerate under climate change. In Sweden, eelgrass of particular concern management and conservation. Here, we provide information on genetic variation, an important component for potential persistence adaptation any changing environment. particular, steep salinity gradient over which distributed along Swedish coast (26 psu west 5 east coast) calls better understanding diversity, connectivity, local adaptation. To assess variation population structure, genotyped individuals with 2,138 single nucleotide polymorphisms (SNPs) from 15 meadows spanning whole distribution. We found geographic structure parallel clear break at entrance Baltic Sea. Meadows low consisted few or only one clone. had higher genotypic richness, showed differentiation smaller scales. With their are especially threatened amidst global changes. Lack sexual reproduction capacity generate new genotypes issue that needs be seriously considered addition, lack renders clonal less recover recolonize after disturbance, more challenging restore. The here provided clusters, clonality, can included prioritizing conservation identifying restoration purposes. Most importantly, monitoring urgently needed temporal changes elsewhere facing

Language: Английский

Citations

5
Genome-wide SNP markers provided insights into the reproductive strategy and genetic diversity of the green tide causative species Ulva prolifera in China DOI

Song Sun,

W. F. Mader, Nan Wang

et al.

Journal of Oceanology and Limnology, Journal Year: 2024, Volume and Issue: 42(2), P. 533 - 543

Published: Jan. 11, 2024

Language: Английский

Citations

1
Unravelling the genetic pattern of seagrass (Posidonia oceanica) meadows in the Eastern Mediterranean Sea DOI Creative Commons
Victoria Litsi‐Mizan, Catalina A. García‐Escudero, Costas S. Tsigenopoulos

et al.

Biodiversity and Conservation, Journal Year: 2023, Volume and Issue: 33(1), P. 257 - 280

Published: Dec. 12, 2023

Abstract The genetic traits of seagrass populations are a crucial aspect their ecology and evolution, influencing adaptability resilience. Here, we studied the diversity, population structure, connectivity eighteen Posidonia oceanica meadows extending in Eastern Mediterranean Sea (Aegean, Ionian Cretan Seas, Greece), combining twelve microsatellite markers Lagrangian particle drift modelling. Our findings revealed strong differentiation between Aegean suggesting limited exchange these two groups. High gene flow was observed within indicating well-connected group populations. Notably, North displayed lowest diversity highest clonality compared to rest lack substantial oceanographic Aegean/Cretan supported differentiation. However, simulations did not fully support patterns Sea, that addition contemporary processes, historical events may have contributed formation pattern. information provided here can be incorporated into management strategies aimed at identifying suitable areas as units conservation efforts determining serve donor sites transplantation initiatives.

Language: Английский

Citations

2
Thermo-priming triggers species-specific physiological and transcriptome responses in Mediterranean seagrasses DOI Creative Commons
Hung Manh Nguyen, Uyen Vu Thuy Hong, Miriam Ruocco

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 210, P. 108614 - 108614

Published: April 7, 2024

Heat priming improves plants tolerance to a recurring heat stress event. The underlying molecular mechanisms of are largely unknown in seagrasses. Here, ad hoc mesocosm experiments were conducted with two Mediterranean seagrass species, Posidonia oceanica and Cymodocea nodosa. Plants first exposed heat-priming, followed by heat-triggering A comprehensive assessment plant response across different levels biological organization was performed at the end triggering Morphological physiological results showed an improved heat-primed P. while C. nodosa both heat- non-primed enhanced their growth rates As resulting from whole transcriptome sequencing, functions related several cellular compartments processes involved warming plants, limited group processes. Our suggest that seagrasses acquire primed state during event, eventually gives ability induce more energy-effective when thermal event recurs. Different species may differ perform after priming. This study provides pioneer insights into emerging topic benefit future studies field.

Language: Английский

Citations

0
A Donor Registry: Genomic Analyses of Posidonia australis Seagrass Meadows Identifies Adaptive Genotypes for Future‐Proofing DOI Creative Commons
Matt J. Nimbs, Tim M. Glasby, Elizabeth A. Sinclair

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT Globally, anthropogenic climate change has caused declines of seagrass ecosystems necessitating proactive restoration approaches that would ideally anticipate future scenarios, such as marine warming. In eastern Australia, estuaries with meadows the endangered Posidonia australi s have warmed and acidified over past decade, communities declined in some estuaries. Securing these valuable habitats will require conservation efforts could be augmented focussed on boosting resilience to climate. Understanding patterns selection where are adapted particular environmental conditions is key for identifying optimal donor material restoration. We used single nucleotide polymorphisms genotype by environment analyses identify candidate loci under putative stressors assess genomic variation allelic turnover along stressor gradients. The most important physicochemical variables driving were associated temperature, water turbidity, pH. developed a preliminary ‘donor registry’ pre‐adapted P. australis genotypes mapping distribution alleles visualise composition each sampled meadow. registry first step select source future‐proofing projects. A next establish manipulative experiments required test whether confer increased resistance multiple stressors.

Language: Английский

Citations

0
Conservation Implications of Strong Population Structure Despite Admixture in an Endangered African Seagrass DOI Creative Commons
Charlotte A. Combrink, Romina Henriques, Matthew Jackson

et al.

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2024, Volume and Issue: 34(12)

Published: Dec. 1, 2024

ABSTRACT Zostera capensis is an African seagrass that endangered throughout its range. In South Africa, it solely confined to low wave energy estuarine habitats and characterised by two evolutionary lineages diverge across a biogeographic transition. this study, we sampled plants from five populations span the region of lineage divergence investigated extent overlap. Using 2681 SNP loci, including 32 putative outlier calculated population structure, genomic diversity levels admixture. All were significantly different each other, those < 10 km apart admixture indicate limited dispersal Z. . Every was high inbreeding coefficient (F IS ), suggesting number breeding individuals in population. Given increasing anthropogenic stressors are linked declines meadow cover our study provides strong support require targeted management conservation actions individual avoid further loss unique dynamics safeguard ecosystem services seagrasses provide. Further, evidence significant structure geographically close highlights efforts relying on restoration would risk mixing signatures when transplanting between estuaries. This represents critical challenge using transplants as potential mechanism restoring declining crucial importance preventing extinction.

Language: Английский

Citations

0
Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants DOI Creative Commons
Pengcheng Wang, Ping Hu, Jinping Zhang

et al.

Avian Research, Journal Year: 2023, Volume and Issue: 14, P. 100078 - 100078

Published: Jan. 1, 2023

Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, has been mostly evaluated through techniques that fail to estimate diversity at whole genomic level. Next generation sequencing can meet this demand, but high quality samples such as blood or muscle tissues are required. it difficult collect from because sampling work may impact their health. Therefore, design a workflow evaluate using non-destructive sampling. Even though used traditional barcoding technique, technique cannot status. Brown Eared Pheasant (Crossoptilon mantchuricum) endangered species, with captive populations maintained Taiyuan Zoo, China, and Europe. diversity, inbreeding pattern, mutation load these two unclear. To uncover populations, we applied 2b-RAD technology feathers samples. The could be collected by results indicate Zoo population lower higher coefficient than European population. lethal mutations when homozygous. current project uses providing paradigm for genetics, which will facilitate development biology.

Language: Английский

Citations

0