Tamarind seed gum-based hydrogel for the targeted delivery of imidazobenzothiazole sulfonamide derivative as an anticancer agent

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139665 - 139665

Published: Jan. 8, 2025

Language: Английский

---

Head and Neck Cancer Immunotherapy: Molecular Biological Aspects of Preclinical and Clinical Research

Cancers, Journal Year: 2023, Volume and Issue: 15(3), P. 852 - 852

Published: Jan. 30, 2023

Breakthrough research in the field of immune checkpoint inhibitors and development a human papilloma virus vaccine triggered plethora cancer immunotherapy. Both had significant effects on treatment head neck squamous cell carcinoma. The advent preclinical models multidisciplinary approaches including bioinformatics, genetic engineering, clinical oncology, immunology helped tumour-infiltrating lymphocytes (TILs) chimeric antigen receptor (CAR) T-cell therapy. Here, we discuss different immunotherapies such as adoptive transfer, inhibitors, interleukins, vaccines for cancer. This review showcases intrinsic relation between understanding implementation basic biology practice. We also address potential limitations each immunotherapy approach advantages personalized Overall, aim this is to encourage further

Language: Английский

---

Evaluating the Impact of Vitamin D₃ on NF-κB and JAK/STAT Signaling Pathways in Drosophila melanogaster

ACS Omega, Journal Year: 2024, Volume and Issue: 9(18), P. 20135 - 20141

Published: April 25, 2024

This study delved into the consequences of prolonged administration vitamin D3 on innate immune systems, particularly NF-κB and JAK/STAT, in Drosophila melanogaster. The outcomes indicated that treatment exhibited a notable capacity to improve survival adult flies with compromised functions, condition induced by loss PGRP-LB, when were exposed heat-killed Escherichia coli. PGRP-LBΔ mutant line was treated E. coli experienced reduced survival. Treatment coli-treated resulted improved survival, this phenotypic feature might be due downregulation gene expression JAK/STAT pathways. However, higher concentration associated decreased potentially linked intricate immunological responses. research also underscored influence antioxidant genes, sod1 sod2, indicating an augmented resistance oxidative stress. Further, revealed effect reproductive status autoinflammatory model, showing increase pupae 10 mM D3, suggesting potential benefits profile. Overall, provides preliminary insights complex interactions between pathways, responses cell, reproduction Drosophila.

Language: Английский

---

Vitamin B6 deficiency cooperates with oncogenic Ras to induce malignant tumors in Drosophila

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(6)

Published: June 3, 2024

Abstract Vitamin B6 is a water-soluble vitamin which possesses antioxidant properties. Its catalytically active form, pyridoxal 5’-phosphate (PLP), crucial cofactor for DNA and amino acid metabolism. The inverse correlation between cancer risk has been observed in several studies, although dietary intake sometimes failed to confirm this association. However, the molecular link remains elusive. Previous work shown that deficiency causes chromosome aberrations (CABs) Drosophila human cells, suggesting genome instability may correlate lack of cancer. Here we provide evidence support hypothesis. Firstly, show PLP deficiency, induced by antagonists 4-deoxypyridoxine (4DP) or ginkgotoxin (GT), promoted tumorigenesis eye larval discs transforming benign Ras V12 tumors into aggressive forms. In contrast, supplementation reduced development tumors. We also low levels, 4DP silencing sgll PNPO gene involved biosynthesis, worsened tumor phenotype another model generated concomitantly activating downregulating Discs-large (Dlg) gene. Moreover, found from larvae reared on displayed CABs, reactive oxygen species (ROS) catalytic activity serine hydroxymethyltransferase (SHMT), PLP-dependent enzyme thymidylate (dTMP) turn required replication repair. Feeding 4DP-fed with ascorbic (AA) plus dTMP, rescued both CABs same effect was produced overexpressing catalase Dlg RNAi larvae, thus allowing establish relationship Overall, our data first vivo demonstration can impact increasing instability, mediated ROS dTMP levels.

Language: Английский

---

Oral administration of plumbagin is beneficial in in vivo models of Duchenne muscular dystrophy through control of redox signaling

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 225, P. 193 - 207

Published: Sept. 24, 2024

Language: Английский

---

Drosophila Model of HPV18-Induced Pathogenesis Reveals a Role for E6 Oncogene in Regulation of NF-κB and Wnt to Inhibit Apoptosis

Tumour Virus Research, Journal Year: 2025, Volume and Issue: unknown, P. 200316 - 200316

Published: March 1, 2025

Cancers caused by high-risk human papillomavirus (HPV) remain a significant health threat resulting in more than 300,000 deaths, annually. Persistent expression of two HPV oncogenes, E6 and E7, are necessary for cancer development progression. has several functions contributing to tumorigenesis one which is blocking programmed cell death, apoptosis. The detailed mechanism anti-apoptosis function not fully understood. Here, using Drosophila model HPV18E6 the UBE3A-induced pathogenesis, we show that anti-apoptotic conserved Drosophila. We demonstrate homologs NF-κB transcription factors, Dorsal Dif proapoptotic. They induce Wingless (Wg, homolog Wnt), leading Our results indicate oncogene inhibits apoptosis downregulating Wg, Dorsal, Dif. Additionally, find Dif, only promote but also regulate autophagy necrosis. promotes while counteracts it, inducing formation acidic vacuoles Interestingly, although blocks proapoptotic it lacks ability interfere with their role apoptosis-independent death. Given high conservation factors our provide new insight into potential mechanisms mediated intervene immortalization action oncoprotein HPV-infected cells.

Language: Английский

---

Exposure to the Natural Compound Climacostol Induces Cell Damage and Oxidative Stress in the Fruit Fly Drosophila melanogaster

Toxics, Journal Year: 2024, Volume and Issue: 12(2), P. 102 - 102

Published: Jan. 24, 2024

The ciliate Climacostomum virens produces the metabolite climacostol that displays antimicrobial activity and cytotoxicity on human rodent tumor cells. Given its potential as a backbone in pharmacological studies, we used fruit fly Drosophila melanogaster to evaluate how xenobiotic affects biological systems vivo at organismal level. Food administration with demonstrated harmful role during larvae developmental stages but not pupation. midgut of eclosed showed apoptosis increased generation reactive oxygen species (ROS), thus demonstrating gastrointestinal toxicity. Climacostol did affect enteroendocrine cell proliferation, suggesting moderate damage does initiate repairing program. fact brain ROS inhibited proliferation neural cells revealed systemic (neurotoxic) this substance. In line, found lower expression relevant antioxidant enzymes impaired mitochondrial activity. Adult offsprings presented no major alterations survival mobility, well absence abnormal phenotypes. However, oviposition behavior was somewhat affected, indicating chronic toxicity climacostol, which continues moderately until adult stages. These results for first time detrimental ingested non-target multicellular organism.

Language: Английский

---

A novel free fatty acid receptor agonist improving metabolic health in Drosophila models

Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107769 - 107769

Published: May 1, 2025

The development of synthetic modulators for human free fatty acid receptors (FFARs) has gained attention addressing diabetes, metabolic syndrome, and dyslipidaemia. A new dual FFAR1 (GPR40) FFAR4 (GPR120) agonist, DFL23916, was recently identified to improve glucose homeostasis, prompting investigations into its effects on disorders. This study assessed the selectivity toxicity DFL23916 in vitro evaluated vivo using Drosophila melanogaster models high-sugar (HSD) high-fat diets (HFD). showed no off-target activities with toxicity/hepatotoxicity cultured cells. In adult HSD-fed flies defective mobility, oral administration enhanced climbing speed a concentration-dependent manner reduced high content triglyceride levels alongside markers insulin resistance. compound did not affect viability food intake exposed chronic overcaloric conditions but it inhibited progressive weight gain. Similarly, larvae remained unaffected by while counteracted elevation lipid droplet size caused HSD HFD. Finally, silico analysis highlighted relevance evolutionary conserved sensing, suggesting putative candidates binding. Collectively, these findings indicated safe profile efficacy ameliorating hyperglycaemic hyperlipidaemic features fed flies. Our results only suggest that could be potential candidate further investigation context disorders also reinforce D. as valuable model preclinical evaluation interventions, including FFAR-targeting strategies.

Language: Английский

---

Using Drosophila melanogaster to Dissect the Roles of the mTOR Signaling Pathway in Cell Growth

Cells, Journal Year: 2023, Volume and Issue: 12(22), P. 2622 - 2622

Published: Nov. 14, 2023

The evolutionarily conserved target of rapamycin (TOR) serine/threonine kinase controls eukaryotic cell growth, metabolism and survival by integrating signals from the nutritional status growth factors. TOR is catalytic subunit two distinct functional multiprotein complexes termed mTORC1 (mechanistic complex 1) mTORC2, which phosphorylate a different set substrates display physiological functions. Dysregulation signaling has been involved in development progression several disease states including cancer diabetes. Here, we highlight how genetic biochemical studies model system

Language: Английский

---

Using Drosophila to uncover the role of organismal physiology and the tumor microenvironment in cancer

Trends in cancer, Journal Year: 2024, Volume and Issue: 10(4), P. 289 - 311

Published: Feb. 13, 2024

Cancer metastasis causes over 90% of cancer patient fatalities. Poor prognosis is determined by tumor type, the microenvironment (TME), organ-specific biology, and animal physiology. While model organisms do not fully mimic complexity humans, many processes can be studied efficiently owing to ease genetic, developmental, cell biology studies. For decades, Drosophila has been instrumental in identifying basic mechanisms controlling growth metastasis. The ability generate clonal populations distinct genotypes otherwise wild-type animals makes a powerful system study tumor–host interactions at local global scales. This review discusses advancements highlighting strength for modeling TMEs systemic responses driving progression

Language: Английский

---