Comprehensive analysis of PRPF19 immune infiltrates, DNA methylation, senescence-associated secretory phenotype and ceRNA network in bladder cancer

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 6, 2023

Background Pre-mRNA processing factor 19 (PRPF19) is an E3 ligase that plays a crucial role in repairing tumor-damaged cells and promoting cell survival. However, the predictive value biological function of PRPF19 bladder urothelial carcinoma (BLCA) require further investigation. Methods In this study, we utilized transcriptomic data cancer tissue microarrays to identify high expression BLCA, suggesting its potential as prognostic biomarker. To gain better understanding immune microenvironment performed single analysis employed LASSO method. Additionally, examined methylation profiles using SMART website. Our investigation confirmed correlation between BLCA senescence stemness. Furthermore, constructed PRPF19-miR-125a-5p-LINC02693-MIR4435-2HG ceRNA network ENCORI miRWALK databases. Results comprehensive reveals can serve marker for significantly associated with various immune-infiltrating BLCA. Moreover, our findings suggest influences cellular through regulation Finally, developed has predict prognosis patients. Conclusion We multiple functions specific be used therapeutic target

Language: Английский

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Proteomic predictors of individualized nutrient-specific insulin secretion in health and disease

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(7), P. 1619 - 1633.e5

Published: July 1, 2024

Population-level variation and mechanisms behind insulin secretion in response to carbohydrate, protein, fat remain uncharacterized. We defined prototypical responses three macronutrients islets from 140 cadaveric donors, including those with type 2 diabetes. The majority of donors' exhibited the highest glucose, moderate amino acid, minimal fatty acid. However, 9% had acid responses, 8% that were larger than their glucose-stimulated responses. leveraged this heterogeneity used multi-omics identify molecular correlates nutrient responsiveness, as well proteins mRNAs altered also examined nutrient-stimulated release stem cell-derived observed responsiveness but not carbohydrate or protein-potentially a hallmark immaturity. Understanding diversity lays groundwork for personalized nutrition.

Language: Английский

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Common immunological and prognostic features of lung and bladder cancer via smoking‐related genes: PRR11 gene as potential immunotherapeutic target

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(10)

Published: May 1, 2024

Abstract Smoking is a well‐known risk factor for non‐small‐cell lung cancer (NSCLC) and bladder urothelial carcinoma (BLCA). Despite this, there has been no investigation into prognostic marker based on smoking‐related genes that could universally predict prognosis in these cancers correlate with immune checkpoint therapy. This study aimed to identify differential NSCLC BLCA, analyse their roles patient therapy through subgroup analyses, shed light PRR11 as crucial gene both cancers. By examining co‐expressed genes, model was constructed its impact immunotherapy BLCA evaluated. Molecular docking tissue microarray analyses were conducted explore the correlation between reciprocal SPDL1. Additionally, miRNAs associated analysed. The confirmed strong link prognosis, BLCA. identified key smoking‐associated influences efficacy of by modulating stemness A established value validated NSCLC. Furthermore, it found regulates PDL1 via SPDL1, impacting immunotherapeutic involvement hsa‐miR‐200b‐3p regulation SPDL1 expression also highlighted. Overall, elucidates modulates influencing interaction potential upstream hsa‐miR‐200b‐3p.

Language: Английский

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Senescence-related gene c-Myc affects bladder cancer cell senescence by interacting with HSP90B1 to regulate cisplatin sensitivity

Aging, Journal Year: 2023, Volume and Issue: unknown

Published: July 10, 2023

Patients with advanced bladder cancer gradually become less sensitive to chemotherapeutic agents, leading tumor recurrence. Initiating the senescence program in solid tumors may be an important means of improving short-term drug sensitivity. The role c-Myc cell was determined using bioinformatics methods. response cisplatin chemotherapy sample analyzed according Genomics Drug Sensitivity Cancer database. Cell Counting Kit-8 assay, clone formation and senescence-associated β-galactosidase staining were used assess growth, senescence, sensitivity cisplatin, respectively. Western blot immunoprecipitation performed understand regulation p21 by c-Myc/HSP90B1. Bioinformatic analysis showed that c-Myc, a cellular gene, significantly associated prognosis chemotherapy. HSP90B1 expression highly correlated cancer. Reducing level inhibited proliferation, promoted enhanced chemosensitivity. Immunoprecipitation assays confirmed interacted c-Myc. reducing could redeem overexpression caused overexpression. Further studies alleviate rapid growth accelerate cells overexpression, levels also improve cells. HSP90B1/c-Myc interaction regulates signaling pathway, which affects chemosensitivity modulating senescence.

Language: Английский

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DET induces apoptosis and suppresses tumor invasion in glioma cells via PI3K/AKT pathway

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 9, 2025

Gliomas, particularly glioblastomas (GBM), are highly aggressive with a poor prognosis and low survival rate. Currently, deoxyelephantopin (DET) has shown promising anti-inflammatory anti-tumor effects. Using clinical prognostic analysis, molecular docking, network pharmacology, this study aims to explore the primary targets signaling pathways identify novel GBM treatment approaches. PharmMapper, chemical structure of DET was examined for possible after being acquired from PubChem. GBM-related were obtained through multi-omics A protein-protein interaction (PPI) constructed using Cytoscape STRING, target binding evaluated docking. Enrichment analysis conducted Metascape. The effects on cell invasion, apoptosis, proliferation assessed in vitro assays, including Transwell, EDU, CCK8, flow cytometry. Western blot performed examine components PI3K/AKT pathway. Among sixty-four shared identified, JUN CCND1 most frequently observed. demonstrated that influenced MAPK pathways. In Transwell significantly inhibited invasive behavior glioma cells. further confirmed downregulation EGFR, JUN, PI3K/AKT. inhibits proliferation, apoptosis via modulating pathway, highlighting its potential as therapeutic strategy treatment.

Language: Английский

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Mechanisms of tumor-associated macrophages in breast cancer and treatment strategy

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Breast cancer (BC) is the most common in women and a leading cause of cancer-related mortality. Despite advances screening treatment, outcomes for advanced or recurrent BC remain poor, highlighting need new strategies. Recent research emphasizes tumor microenvironment (TME), particularly tumor-associated macrophages (TAMs), as key drivers growth, metastasis, resistance to therapy. The presence M2-like TAMs TME promotes immune evasion progression across subtypes. This review summarizes classification, their role BC, emerging therapies targeting TAMs, including depletion, inhibition recruitment, reprogramming from pro-tumoral M2 anti-tumoral M1 phenotypes. Targeting offers promising strategy improve treatment outcomes.

Language: Английский

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New progress in imaging diagnosis and immunotherapy of breast cancer

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Breast cancer (BC) is a predominant malignancy among women globally, with its etiology remaining largely elusive. Diagnosis primarily relies on invasive histopathological methods, which are often limited by sample representation and processing time. Consequently, non-invasive imaging techniques such as mammography, ultrasound, Magnetic Resonance Imaging (MRI) indispensable for BC screening, diagnosis, staging, treatment monitoring. Recent advancements in technologies artificial intelligence-driven radiomics have enhanced precision medicine enabling early detection, accurate molecular subtyping, personalized therapeutic strategies. Despite reductions mortality through traditional treatments, challenges like tumor heterogeneity resistance persist. Immunotherapies, particularly PD-1/PD-L1 inhibitors, emerged promising alternatives. This review explores recent developments diagnostics immunotherapeutic approaches, aiming to inform clinical practices optimize outcomes.

Language: Английский

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Targeting tumor-associated macrophages in colon cancer: mechanisms and therapeutic strategies

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Colon cancer (CC) remains a primary contributor to cancer-related fatalities worldwide, driven by difficulties in early diagnosis and constrained therapeutic options. Recent studies underscore the importance of tumor microenvironment (TME), notably tumor-associated macrophages (TAMs), fostering malignancy progression therapy resistance. Through their inherent plasticity, TAMs facilitate immunosuppression, angiogenic processes, metastatic spread, drug tolerance. In contrast M1 macrophages, which promote inflammatory tumoricidal responses, M2 support expansion dissemination exerting immunosuppressive pro-angiogenic influences. Consequently, manipulating has emerged as potential avenue enhance treatment effectiveness. This review outlines origins, polarization states, functions CC, highlights role driving advancement, surveys ongoing efforts target these cells for better patient outcomes. Emerging strategies aimed at modulating TAM - including depletion strategies, reprogramming approaches that shift M2-polarized toward an phenotype, inhibition key signaling pathways sustaining TAM-mediated immunosuppression-are currently under active investigation. These hold promise overcoming induced resistance improving immunotherapeutic efficacy CC.

Language: Английский

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Molecular mechanisms and signaling pathways related to brain metastasis in breast cancer

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 27, 2025

Brain metastasis in breast cancer (BCBM) significantly threatens the survival and quality of life patients, particularly those with triple-negative (TNBC) HER2-positive subtypes. It involves complex molecular mechanisms diverse signaling pathways. This review highlights recent research on pathways BCBM. The process BCBM includes several key steps: local infiltration cells into bloodstream subsequent spread to brain. They must then overcome blood-brain barrier (BBB) establish grow Multiple pathways, including PI3K/AKT, STAT3, NF-κB, Notch, Wnt are involved this process. Overall, is a disease regulated by multiple To improve patient life, it crucial deepen explore new treatment targets strategies. will enhance our understanding lead more effective treatments.

Language: Английский

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Immune microenvironment and immunotherapy in hepatocellular carcinoma: mechanisms and advances

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 2, 2025

Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality globally. The tumor microenvironment (TME) plays pivotal role in HCC progression, characterized by dynamic interactions between stromal components, immune cells, and cells. Key players, including tumor-associated macrophages (TAMs), tumor-infiltrating lymphocytes (TILs), cytotoxic T (CTLs), regulatory cells (Tregs), MDSCs, dendritic (DCs), natural killer (NK) contribute to evasion progression. Recent advances immunotherapy, such as checkpoint inhibitors (ICIs), cancer vaccines, adoptive cell therapy (ACT), combination therapies, have shown promise enhancing anti-tumor responses. Dual ICI combinations, ICIs with molecular targeted drugs, integration local treatments or radiotherapy demonstrated improved outcomes patients. This review highlights the evolving understanding therapeutic potential immunotherapeutic strategies management.

Language: Английский

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