ABSTRACT
Sorghum
(
bicolor
(L.)
Moench)
is
a
highly
nutritional
multipurpose
millet
crop.
However,
the
genetic
and
molecular
regulatory
mechanisms
governing
sorghum
grain
development
associated
agronomic
traits
remain
unexplored.
In
this
study,
we
performed
comprehensive
transcriptomic
analysis
of
pistils
collected
1–2
days
before
pollination,
developing
seeds
–2,
10,
20
30
after
pollination
S.
variety
M35‐1.
Out
31
337
genes
expressed
in
these
stages,
12
804
were
differentially
consecutive
stages
seed
development.
These
exhibited
10
dominant
expression
patterns
correlated
with
distinct
pathways
gene
functions.
Functional
analysis,
based
on
pathway
mapping,
transcription
factor
enrichment
orthology,
delineated
key
fertilization,
early
development,
filling
maturation.
Furthermore,
colocalization
previously
reported
quantitative
trait
loci
(QTLs)
for
weight/size
revealed
48
mapping
to
QTL
regions.
Comprehensive
literature
mining
integrated
data
shortlisted
25,
17
8
core
candidates
engineering
size,
starch
protein
content,
respectively.
Plants,
Journal Year:
2024,
Volume and Issue:
13(7), P. 977 - 977
Published: March 29, 2024
A
newly
documented
pathotype
5
of
the
soil-borne
fungus
Sporisorium
reilianum,
causing
head
smut
in
sorghum,
was
tested
against
153
unexplored
Senegalese
sorghum
accessions.
Among
accessions
tested,
63
(41%)
exhibited
complete
resistance,
showing
no
signs
infection
by
fungus.
The
remaining
90
(59%)
displayed
varying
degrees
susceptibility.
Sorghum
responses
S.
reilianum
were
explored
to
analyze
potential
link
with
previously
known
seed
morphology-related
traits
and
new
phenotype
data
from
59
lines
for
weight.
genome-wide
association
study
(GWAS)
screened
297,876
SNPs
identified
highly
significant
associations
(p
<
1
×
10−5)
resistance
sorghum.
By
mapping
these
reference
genome,
this
revealed
35
novel
candidate
defense
genes
potentially
involved
disease
resistance.
Frontiers in Bioscience-Landmark,
Journal Year:
2023,
Volume and Issue:
28(10), P. 233 - 233
Published: Sept. 28, 2023
Background:
Fallopia
aubertii
(L.
Henry)
Holub
is
a
perennial
semi-shrub
with
both
ornamental
and
medicinal
value.
The
mitochondrial
genomes
of
plants
contain
valuable
genetic
traits
that
can
be
utilized
for
the
exploitation
resources.
parsing
F.
genome
provide
insight
into
role
mitochondria
in
plant
growth
development,
metabolism
regulation,
evolution,
response
to
environmental
stress.
Methods:
In
this
study,
we
sequenced
using
Illumina
NovaSeq
6000
platform
Nanopore
platform.
We
conducted
comprehensive
analysis
aubertii,
which
involved
examining
various
aspects
such
as
gene
composition,
repetitive
sequences,
RNA
editing
sites,
phylogeny,
organelle
homology.
To
achieve
this,
employed
several
bioinformatics
methods
including
sequence
alignment
analysis,
phylogeny
more.
Results:
has
64
genes,
34
protein-coding
genes
(PCGs),
three
rRNAs,
27
tRNAs.
There
were
77
short
tandem
repeat
sequences
detected
genome,
five
identified
by
Tandem
Repeats
Finder
(TRF),
50
scattered
observed,
22
forward
28
palindrome
sequences.
A
total
367
coding
sites
predicted
PCGs,
highest
number
(33)
found
within
ccmB.
Ka/Ks
values
estimated
closely
related
species
representing
Caryophyllales
less
than
1
most
genes.
maximum
likelihood
evolutionary
tree
showed
Nepenthes
×ventrata
are
related.
Conclusions:
obtained
basic
information
on
study
investigated
homologous
segments,
ratio
estimate
selection
pressure
aubertii.
also
discussed
systematic
position
based
Our
revealed
variations
structure
Caryophyllales.
These
findings
great
significance
identifying
improving
serve
reference
future
molecular
studies
ABSTRACT
Sorghum
(
bicolor
(L.)
Moench)
is
a
highly
nutritional
multipurpose
millet
crop.
However,
the
genetic
and
molecular
regulatory
mechanisms
governing
sorghum
grain
development
associated
agronomic
traits
remain
unexplored.
In
this
study,
we
performed
comprehensive
transcriptomic
analysis
of
pistils
collected
1–2
days
before
pollination,
developing
seeds
–2,
10,
20
30
after
pollination
S.
variety
M35‐1.
Out
31
337
genes
expressed
in
these
stages,
12
804
were
differentially
consecutive
stages
seed
development.
These
exhibited
10
dominant
expression
patterns
correlated
with
distinct
pathways
gene
functions.
Functional
analysis,
based
on
pathway
mapping,
transcription
factor
enrichment
orthology,
delineated
key
fertilization,
early
development,
filling
maturation.
Furthermore,
colocalization
previously
reported
quantitative
trait
loci
(QTLs)
for
weight/size
revealed
48
mapping
to
QTL
regions.
Comprehensive
literature
mining
integrated
data
shortlisted
25,
17
8
core
candidates
engineering
size,
starch
protein
content,
respectively.
