Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus DOI Creative Commons
Ting Chen,

Yun Yang,

Xuan Wang

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 18, 2024

In this study, a chromosome-level genome of the tropical sea cucumber Stichopus monotuberculatus was generated by combination Nanopore long-read, Illumina short-read, and Hi-C sequencing technologies. The final assembly 810.54 Mb in length, with contig N50 scaffold values 10.15 35.36 Mb, respectively. This comprised 23 pseudo-chromosomes, covering 99.82% genome. Completeness analysis using BUSCO indicated that 97.8% metazoan conserved genes were presented their entirety. A total 29,596 protein-coding predicted, functional annotations available for 94.43% these genes. high-quality produced study may provide an essential foundation future researches on resource conservation genetic breeding S. monotuberculatus.

Language: Английский

Diversity, mechanism and structure-activity relationships of marine anticoagulant-active polysaccharides: A review DOI
Kaiqiang Li, Rongfeng Li, Yuanjie Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141742 - 141742

Published: March 1, 2025

Language: Английский

Citations

0

Chromosomal-level genome assembly and annotation of the tropical sea cucumber Holothuria scabra DOI Creative Commons
Shengping Zhong, Xujia Liu, Xiaowan Ma

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: May 9, 2024

Abstract Holothuria scabra , a commercially valuable yet ecologically vulnerable tropical holothuroid, has experienced severe decline in its wild populations, especially China. Genomic resources are crucial for the development of effective genomic breeding projects and stock conservation strategies to restore these natural populations. Until now, high-quality, chromosome-level reference genome H. not been available. Here, we employed Oxford Nanopore Hi-C sequencing technologies assemble annotate . The final comprised 31 scaffolds with total length 1.19 Gb scaffold N50 53.52 Mb. Remarkably, 1,191.67 Mb (99.95%) sequences were anchored 23 pseudo-chromosomes, longest one spanning 79.75 A 34,418 protein-coding genes annotated genome, BUSCO analysis revealing 98.01% coverage metazoa_odb10 genes, marking significant improvement compared previous report. These annotations will provide an essential basis further investigation into molecular management

Language: Английский

Citations

3

Sea cucumbers: an emerging system in evo-devo DOI Creative Commons
Margherita Perillo, Rosa Maria Sepe, Periklis Paganos

et al.

EvoDevo, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 17, 2024

A challenge for evolutionary developmental (evo-devo) biology is to expand the breadth of research organisms used investigate how animal diversity has evolved through changes in embryonic development. New experimental systems should couple a relevant phylogenetic position with available molecular tools and genomic resources. As phylum sister group chordates, echinoderms extensively contributed our knowledge patterning, organ development cell-type evolution. Echinoderms display variety larval forms diverse shapes, making them suitable compare evolution strategies. However, because laboratory accessibility already techniques, most studies focus on sea urchins stars mainly. comparative approach, field would benefit from including information other members this group, like cucumbers (holothuroids), which little known basis their Here, we review spawning culture methods, morphological information, current state transcriptomic resources cucumbers. With goal system accessible broader community, discuss cucumber embryos larvae can be powerful address open questions evo-devo, understanding origins bilaterian structures.

Language: Английский

Citations

2

Development and application of molecular markers in fisheries, aquaculture, and industry of representative temperate and tropical sea cucumbers: a review DOI Creative Commons
Xinghai Liu,

Xinyue Tang,

Muyan Chen

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: June 25, 2024

Sea cucumber has emerged as a crucial economic species in aquaculture China because of its remarkable nutritional and medicinal value. However, wild sea populations have experienced decline due to overfishing environmental factors, underscoring the urgent need for genetic resource conservation biotechnology innovation within breeding industry. The development industry is still impeded by challenges difficulties. Nevertheless, significant progress been made through utilization molecular markers, which effectively addressed number fisheries issues. In recent years, diverse types markers including mitochondrial DNA, microsatellites, SNP developed extensively applied various aspects research. These play vital roles sex identification, germplasm evaluation, population structure assessment, well marker-assisted marine ranching This review provides an overview fundamental principles, functions, characteristics associated with employed across while also discussing their applications different fisheries, aquaculture,

Language: Английский

Citations

2

Genome variations in sea cucumbers: Insights from genome survey sequencing and comparative analysis of mitochondrial genomes DOI

Chunxi Jiang,

Hongsheng Yang,

Bohong Liu

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 52, P. 101328 - 101328

Published: Sept. 15, 2024

Language: Английский

Citations

0

Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus DOI Creative Commons
Ting Chen,

Yun Yang,

Xuan Wang

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 18, 2024

In this study, a chromosome-level genome of the tropical sea cucumber Stichopus monotuberculatus was generated by combination Nanopore long-read, Illumina short-read, and Hi-C sequencing technologies. The final assembly 810.54 Mb in length, with contig N50 scaffold values 10.15 35.36 Mb, respectively. This comprised 23 pseudo-chromosomes, covering 99.82% genome. Completeness analysis using BUSCO indicated that 97.8% metazoan conserved genes were presented their entirety. A total 29,596 protein-coding predicted, functional annotations available for 94.43% these genes. high-quality produced study may provide an essential foundation future researches on resource conservation genetic breeding S. monotuberculatus.

Language: Английский

Citations

0