Intercropping of Saccharum spp. with Dictyophora indusiata: effects on microbial communities and metabolite profiles during bagasse degradation

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Background Intercropping Saccharum spp. (sugarcane) with the fungus Dictyophora indusiata together bagasse amendment represents an innovative circular agriculture method that can enhance soil health, boost sugarcane yields, and increase farm profitability. Understanding process by which D. degrades is key to optimizing this method. Aims This study aims clarify microbial metabolic processes involved in degradation intercropping system. Methods Chemical composition analysis, metabarcoding sequencing, metabolomic profiling were conducted on -degraded (DIBA) naturally degraded (BA). Results Analysis of chemical revealed only acid detergent fiber (ADF) crude protein content differed significantly between DIBA BA treatments. Metabarcoding sequencing showed altered bacterial fungal communities, reducing diversity. Metabolomic analysis indicated enhancement biological metabolism, particularly carbohydrate breakdown, treatment. Key metabolites, such as glucose, cellobiose, D-mannose, more abundant samples. In addition, unique metabolites L-alanine, serine, oxaloacetate detected treatment, suggesting efficient compared natural processes. Conclusion The use macrofungi play a pivotal role transforming agricultural waste into valuable amendments. Future research should focus long-term impact system quality crop yield, well underlying mechanisms, further optimize systems fungi management.

Language: Английский

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Investigation of the Impact of Soil Physicochemical Properties and Microbial Communities on the Successful Cultivation of Morchella in Greenhouses

Horticulturae, Journal Year: 2025, Volume and Issue: 11(4), P. 356 - 356

Published: March 26, 2025

Morels (Morchella spp.) are medicinal and edible mushrooms, renowned for their distinctive taste appearance. Due to the low yields difficulty of foraging wild morels, artificial cultivation has significant economic value. Outdoor influenced by factors such as weather diseases, which can result in crop instability or failure, thereby causing losses farmers. Previous studies have typically concentrated on either fungal bacterial communities. In this study, we investigated ecological relationships between morel growth both fungi bacteria soil, analyzed over multiple trophic levels. We three soil types: death was observed (DM), no morels emerged (UM), that is suitable normal fruiting (NM). used high-throughput ITS 16S rDNA amplicon sequencing, alongside assessment physicochemical properties, investigate contributing emergence death. The results indicated richness diversity communities (NM) were significantly higher than those non-fruiting soils (DM UM). community primarily composed Proteobacteria Bacteroidota, while dominated Ascomycota Mucoromycota. Furthermore, Morchella enriched NM, indicating it had successfully colonized could develop into bodies. mycelium NM effectively utilized external nutrient bags, enhancing nitrogen organic matter content reducing consumption available phosphorus potassium. LEfSe random forest analyses identified Pedobacter Massilia biomarkers potentially associated with symbiosis Morchella, may promote its growth. construction fungal-bacterial co-occurrence network revealed exhibited a number nodes greater stability, suggesting complex microbial structure play crucial role successful Morchella. Our indicate failures production due inadequate management practices. Elevated greenhouse temperatures promoted pathogen proliferation, hindering effective utilization bags mycelium. Consequently, unable accumulate nutrients efficiently, leading inability fruit resulting developmental failures. This study offers valuable insights interactions microorganisms, elucidating reasons failure strategies optimizing cultivation.

Language: Английский

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Microbial Community Response and Assembly Process of Yellow Sand Matrix in a Desert Marginal Zone Under Morchella Cultivation

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 921 - 921

Published: April 16, 2025

In this study, we investigated the adaptation of yellow-sand-substrate Morchella cultivation in desert fringe and its effect on soil physicochemical properties microbial communities. The qPCR high-throughput sequencing with null modeling analyzed diversity, networks, assembly under nutrient supplementation, linking changes to dynamics. results showed that yellow sand substrate can be planted area, as bags resulted a yield 691 g/m2 fruit units. Cultivation could significantly increase substrate, such organic matter (SOM), total nitrogen (TN), ammonium (NH4+−N), amount carbon (MBC/MBN). fungal community was dominated by Ascomycota, Basidiomycota, Firmicutes, Bacteroidota, Actinobacteriota. RDA analysis Ascomycota Proteobacteria were positively correlated NH4+−N, MBN, SOM, MBC, acting potassium (AK), TN, C/N. promoted positive correlation-dominant network pattern substrate. bag treatment reduced bacterial complexity while enhancing complexity, connectivity stability, accompanied significant increases Proteobacteria, Cladosporium, Thermomyces relative abundances during until original degradation. Deterministic processes communities, morel drove toward heterogeneous selection processes. study revealed economic value application potential improving sandy soil, which is great importance for practical mushrooms desert.

Language: Английский

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Recruitment and Aggregation Capacity of Tea Trees to Rhizosphere Soil Characteristic Bacteria Affects the Quality of Tea Leaves

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1686 - 1686

Published: June 18, 2024

There are obvious differences in quality between different varieties of the same plant, and it is not clear whether they can be effectively distinguished from each other a bacterial point view. In this study, 44 tea tree (Camellia sinensis) were used to analyze rhizosphere soil community using high-throughput sequencing technology, five types machine deep learning for modeling obtain characteristic microorganisms that differentiate varieties, validation was performed. The relationship microorganisms, nutrient transformation, formation further analyzed. It found classified into two groups (group A group B) bacteria them came 23 genera. Secondly, content available nutrients (available nitrogen, phosphorus, potassium) indexes (tea polyphenols, theanine, caffeine) significantly higher than B. classification result based on both consistent with above bacteria. This study provides new insight research methodology main reasons among plant.

Language: Английский

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Comparison of Bacterial Communities in Five Ectomycorrhizal Fungi Mycosphere Soil

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1329 - 1329

Published: June 29, 2024

Ectomycorrhizal fungi have huge potential value, both nutritionally and economically, but most of them cannot be cultivated artificially. To better understand the influence abiotic biotic factors upon growth ectomycorrhizal fungi, mycosphere soil bulk five (

Language: Английский

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Diversity and co-occurrence patterns of soil bacterial and fungal communities in two morels

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

This study investigates the influence of different morels growth on soil properties, microbial communities, and cultivation outcomes. Through comprehensive analysis, we found that M. sextelata septimelata significantly affect physical chemical including pH effective content carbon (C), nitrogen (N), potassium (K). While both species exhibit similar effects nutrient enhancement, displays superior reduction disease resistance. Microbial community analysis revealed significant differences between sextelataand septimelata, with sextelatashowing a higher abundance certain bacterial taxa, indicating resilience to environmental stress. Co-occurrence network demonstrates complexity interactions, exhibiting more intricate network. Moreover, phosphorus levels play crucial role in shaping fungal structure. Understanding these relationships is vital for morel ecosystem management strategies.

Language: Английский

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Unraveling the key mechanisms of Gastrodia elata continuous cropping obstacles: soil bacteria Massilia, Burkholderia-Caballeronia-Paraburkholderia, and Dyella along with soil metabolites 4-hydroxy-benzenemethanol and N-(2-butyl)-N-octadecyl-, ethyl ester as crucial indicators

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 28, 2024

Background Tian-ma ( Gastrodia elata ) is a traditional medicinal herb found in China. It used healthy food and to treat various diseases, therefore cultivated extensively southwest However, continuous cropping of this species has led obstacles, such as microbial disease pest infestation, significantly affecting the production development valuable resources. As per growth habit, soil presumed be primary factor contributing these despite known issues obstacles , disease, there lack comprehensive understanding specific bacterial communities metabolites involved processes. Methods We analyzed samples collected during year cultivation (0 Year), after harvest (1 two years (2 three (3 Year) fallowing post-cultivation using 16S rRNA metabarcoding sequencing by illumina platform metabolomics (GC–MS/MS). Soil sample from uncultivated field was control (CK). Results Metabarcoding showed high alpha diversity period deterioration community. with decreased anaerobic abundance increased copiotrophic abundance. Bacteria associated sulfur metabolism also obstacles. Further approach identified 4-hydroxy-benzenemethanol an indicator Besides, carbohydrate class were most abundant occurrence suggesting that regulation may critical addressing Finally, correlation analysis indicated positive association between some metabolite, e.g., carbamic acid, N-(2-butyl)-N-octadecyl-, ethyl ester detected bacteria capable degrading toxic metabolites, Massilia Burkholderia-Caballeronia-Paraburkholderia Dyella . Conclusion This study revealed metabolic factors related These findings not only deepen our but pave way for developing effective strategies overcome them.

Language: Английский

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Challenges and Strategies for Continuous Cropping of Morchella spp.: A Review

Horticulturae, Journal Year: 2024, Volume and Issue: 10(12), P. 1288 - 1288

Published: Dec. 3, 2024

Continuous cropping obstacles (CCOs) have increasingly become an important phenomenon affecting morel mushroom yield and quality. In this review, we summarize the current knowledge about driving factors behind continuous cropping, including increase in soil nutrients, disorder of microbial structure, aggravation diseases, accumulation allelochemicals autotoxins. Furthermore, analyze interrelationships among these factors. To mitigate adverse effects, propose five potential strategic approaches aimed at optimizing existing interventions. This overview not only delineates causative agents their intricate interactions under scenarios but also consolidates prospective solutions to alleviate constraints, thereby advancing our understanding management production sustainability.

Language: Английский

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