Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155408 - 155408
Published: June 13, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(28)
Published: April 19, 2024
Abstract Ulcerative colitis (UC) is a complicated and recurrent intestinal disease. Currently available drugs for UC treatment are scarce, therefore, novel therapeutic the urgently to be developed. Gingerenone A (GA) phenolic compound known its anti‐inflammatory effect, but effect on remains unknown. Here, it shown that GA protects mice against UC, which closely associated with inhibiting mucosal inflammation enhancing barrier integrity in vivo vitro. Of note, RNA sequencing analysis demonstrates an evident correlation IL‐17 signaling pathway after treatment, this further corroborated by Western blot. Mechanistically, directly interacts IL‐17RA protein through pull‐down, surface plasmon resonance molecular dynamics simulation. Importantly, lentivirus‐mediated IL‐17RA/Act1 knock‐down or co‐treatment brodalumab/ixekizumab significantly impairs protective effects of DSS‐induced dysfunction, suggesting critical role GA‐mediated protection UC. Overall, these results indicate effective agent mainly direct binding inhibit inflammatory activation.
Language: Английский
Citations
31
CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(4)
Published: April 1, 2024
Abstract The etiology of epilepsy is ascribed to the synchronized aberrant neuronal activity within brain. Circular RNAs (circRNAs), a class non‐coding characterized by their circular structures and covalent linkage, exert substantial influence on this phenomenon. CircRNAs possess stereotyped replication, transience, repetitiveness, paroxysm. Additionally, MicroRNA (miRNA) plays crucial role in regulation diverse pathological processes, including epilepsy. CircRNA particular significance due its ability function as competing endogenous RNA, thereby sequestering or inhibiting miRNA through binding target mRNA. Our review primarily concentrates elucidating functional roles, well underlying mechanisms, circRNA–miRNA–mRNA networks it explores potential utility these for early detection therapeutic intervention.
Language: Английский
Citations
19
Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)
Published: Aug. 17, 2024
tRNA-derived small RNAs (tsRNAs) are newly discovered non-coding RNA, which generated from tRNAs and reported to participate in several biological processes diseases, especially cancer; however, the mechanism of tsRNA involvement colorectal cancer (CRC) 5-fluorouracil (5-FU) is still unclear.
Language: Английский
Citations
16
Cellular Signalling, Journal Year: 2025, Volume and Issue: 127, P. 111605 - 111605
Published: Jan. 20, 2025
Language: Английский
Citations
1
Infectious Agents and Cancer, Journal Year: 2025, Volume and Issue: 20(1)
Published: Feb. 7, 2025
Colorectal cancer (CRC) ranks among the frequently occurring malignant neoplasms affecting gastrointestinal tract. This study aimed to explore JAK-STAT signaling pathway related genes in CRC and establish a new prognostic model. The data set used this is from public database. JAK-STAT-differentially expressed (DEGs) were identified through differential expression analysis weighted gene co-expression network (WGCNA). Prognostic selected JAK-STAT-DEGs Mendelian randomization (MR), univariate Cox regression, least absolute shrinkage selection operator (LASSO) analyses. expressions of verified by RT-qPCR. Then, risk model was built validated GSE39582. Independent factors screened underlying scores different clinical indicators, resulting construction nomogram. Additionally, immune infiltration, checkpoint inhibitors analyses enrichment (GSEA) carried out. 3,668 obtained intersection 5826 CRC-DEGs 9766 key module genes. Five (ANK3, F5, FAM50B, KLHL35, MPP2), their significantly between control groups. A constructed according In addition, nomogram exhibited superior predictive accuracy for CRC. Furthermore, results indicated notable positive correlation score (R = 0.486), stromal 0.309), ESTIMATE 0.422). Immune inhibitor ADORA2A (Cor 0.483263) strongest with score. And MPP2 most potent activating influence on cell cycle pathway, whereas ANK3 demonstrated significant inhibitory effect within apoptosis pathway. validated, which possessed an potential patients' prognosis could potentially enhance tailored guidance immunotherapy.
Language: Английский
Citations
1
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)
Published: Feb. 1, 2025
ABSTRACT Colorectal carcinoma (CRC) poses a serious risk to global human health. Long non‐coding RNAs (LncRNAs) play an important role in the pathogenesis of CRC. There is scarcity data about newly identified lncRNA, FAM30A. Our major objective investigate FAM30A process Gene expression and correlated clinical information were retrieved downloaded from public databases identify differentially expressed genes linked The was samples CRC cell lines using via Quantitative Real‐time Polymerase Chain Reaction (qPCR) assay also. survival significance determined R package “survival.” Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis performed FAM30A‐related signalling pathway. levels proteins by western blot assay. effect on biological behaviours evaluated function experiments. down‐regulated based database. had lower lines. Low positively related poor prognosis patients. After overexpressed, proliferation, invasion, migration abilities cells decreased, rate apoptosis increased. Furthermore, overexpression could block JAK–STAT signalling. suppresses proliferative, invasive, migratory through blocking Thus, it can be novel biomarker prognosis.
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 2, 2025
Language: Английский
Citations
0
Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: 266, P. 155810 - 155810
Published: Jan. 5, 2025
Language: Английский
Citations
0
Human Molecular Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 7, 2025
Abstract This study systematically explores the oncogenic role of long non-coding RNA (lncRNA) LINC00115 in endometrial cancer (EC) and reveals its unique mechanism promoting proliferation, invasion, metastasis via JAK/STAT signaling pathway. is significantly upregulated EC tissues closely associated with advanced TNM staging lymph node metastasis. Functional assays showed that knockdown suppressed cell metastasis, while overexpression enhanced these malignant behaviors. In vivo models confirmed accelerates tumor growth Our first to identify as a key activator pathway through direct interaction KH-type Splicing Regulatory Protein (KHSRP), previously unrecognized EC. finding provides new insights into lncRNA-mediated regulation highlights novel biomarker promising therapeutic target for EC, offering theoretical basis developing targeted therapies.
Language: Английский
Citations
0
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(1)
Published: Jan. 1, 2025
As the most prevalent subtype of lung cancer, adenocarcinoma (LUAD) is closely associated with angiogenesis, which fundamental to its progression. ADAM8 (A disintegrin and metalloproteinase 8) an enzyme tumor invasion, while implications in LUAD angiogenesis are a field that awaits exploration. A thorough investigation into impacts on could contribute development therapeutic drugs for LUAD. Bioinformatics delineated expression profiles TFAP2A tissues, focusing ADAM8-enriched pathways. qRT-PCR confirmed their cells. The CCK-8 assay was applied gauge cell viability, Western blot detected presence JAK2/STAT3 pathway proteins VEGFR-2 VEGF. Angiogenesis assays quantified length dual-luciferase Chromatin immunoprecipitation verified TFAP2A-ADAM8 binding. exhibited high tissues cells, notable enrichment VEGF JAK/STAT Cellular revealed elevated enhanced promoted phosphorylation JAK2 STAT3, boosted angiogenic capacity. JAK inhibitor Peficitinib reversed proangiogenic effects induced by overexpression. We also discovered overexpression TFAP2A, upstream transcription factor ADAM8, Rescue experiments indicated counteract inhibitory knockdown angiogenesis. This study reveals first time critical role demonstrating promotes conduction activating ADAM8. finding not only provides new perspective understanding pathogenesis but lays foundation therapies targeting
Language: Английский
Citations
0